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Application of hnRNPA1 in diagnosis, prognosis and treatment of pancreatic cancer

A technology for pancreatic cancer and prognosis, applied in the field of biomedicine, can solve the problem of the lack of efficient biomarkers and clinical treatment targets for patients with lymphatic metastasis of pancreatic cancer, and achieve the promotion of lymphangiogenesis and lymphatic metastasis, and short disease-free survival rate. Effect

Pending Publication Date: 2022-03-11
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, more and more basic research results have given us an in-depth understanding of the molecular mechanism of lymphatic metastasis of pancreatic cancer, but there is still a lack of efficient biomarkers and clinical therapeutic targets in the diagnosis and treatment of patients with lymphatic metastasis of pancreatic cancer

Method used

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  • Application of hnRNPA1 in diagnosis, prognosis and treatment of pancreatic cancer
  • Application of hnRNPA1 in diagnosis, prognosis and treatment of pancreatic cancer
  • Application of hnRNPA1 in diagnosis, prognosis and treatment of pancreatic cancer

Examples

Experimental program
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Effect test

Embodiment 1

[0039] hnRNPA1 is highly expressed in serum exosomes from patients with pancreatic cancer

[0040] The inventor firstly collected the serum samples of 5 patients with pancreatic cancer and 5 normal healthy volunteers, transferred the supernatant to a new centrifuge tube by ultracentrifugation (2000g × 20 min centrifugation, then centrifuged at 10000g × 40 min and then transferred Transfer the supernatant to a new centrifuge tube, then centrifuge at 120,000g X 70 min, discard the supernatant, and resuspend the exosomes at the bottom of the tube with PBS) to extract and purify the exosomes in the serum, and then screen them by proteomic analysis The protein hnRNPA1 was found to be highly expressed in serum exosomes of patients with pancreatic cancer.

[0041] The inventor expanded clinical samples, collected serum samples from 186 patients with pancreatic cancer and 186 healthy volunteers, extracted the serum exosomes, and identified the extracted cysts by scanning electron micr...

Embodiment 2

[0045]Relationship between exosomal hnRNPA1 expression and disease-free survival and overall survival in patients with pancreatic cancer:

[0046] The clinical information of all pancreatic cancer patients was collected and combined with the above qRT-PCR test results for statistical analysis. It was found that the expression of serum exosomal hnRNPA1 in pancreatic patients with lymph node metastasis was significantly higher than that in patients without lymph node metastasis. And patients with high expression of serum exosomal hnRNPA1 had shorter disease-free survival and overall survival ( Figure 4 , Analysis method: Kaplan–Meier survival curve analysis, the expression of exosomal hnRNPA1 above the median is defined as high expression of exosomal hnRNPA1, and the following is defined as low expression of exosomal hnRNPA1).

[0047] The above results show that the subjects with high expression of exosomal hnRNPA1 have shorter overall survival than those with low expression o...

Embodiment 3

[0050] Diagnostic efficacy of exosomal hnRNPA1 for pancreatic cancer:

[0051] By detecting the expression of serum exosomal hnRNPA1, combined with receiver operating characteristic (ROC) analysis, it was found that:

[0052] Serum exosomal hnRNPA1 had good diagnostic performance for pancreatic cancer, with an area under the curve (AUC) of 0.85 (95% confidence interval: 0.81-0.89), which was significantly higher than that of CEA (AUC: 0.77; 95%CI: 0.72-0.82 ) or CA72-4 (AUC: 0.68; 95%CI: 0.63-0.73), basically the same as CA19-9 (AUC: 0.90; 95% CI: 0.87-0.94), see Figure 5 .

[0053] The combined application of CA19-9 and exosomal hnRNPA1 has a better diagnostic performance for pancreatic cancer than that of CA19-9 alone. (95%CI: 0.87-0.94), see Image 6 .

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Abstract

The invention discloses an application of hnRNPA1 in diagnosis, prognosis and treatment of pancreatic cancer, the application of hnRNPA1 as a molecular marker in pancreatic cancer finds that hnRNPA1 is highly expressed in a blood exosome of a pancreatic cancer patient, a subject with high expression of the exosome hnRNPA1 has a shorter total lifetime than a subject with low expression of the exosome hnRNPA1, and the hnRNPA1 can be applied to diagnosis, prognosis and treatment of pancreatic cancer. A subject with high expression of the exosome hnRNPA1 has a shorter disease-free lifetime than a subject with low expression of the exosome hnRNPA1; experiments discover that the exosome hnRNPA1 can significantly promote lymphatic neogenesis and lymphatic metastasis of pancreatic cancer, which prompts that the exosome hnRNPA1 can be used as a molecular marker for diagnosis and prognosis of pancreatic cancer and a new target for treatment of pancreatic cancer.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an application of hnRNPA1 in the diagnosis, prognosis and treatment of pancreatic cancer. Background technique [0002] Pancreatic cancer is one of the most malignant tumors in solid tumors. It has the characteristics of insidious onset, low surgical resection rate, strong local invasion, easy metastasis in the early stage, easy recurrence after operation, and extremely poor overall prognosis. In recent years, with the changes in residents' diet structure and lifestyle and the increase of clinical detection rate, the incidence of pancreatic cancer has increased year by year, seriously threatening the lives and health of residents. It is estimated that in 2020, the number of new cases of pancreatic cancer in the world will reach 420,000, and the number of deaths will reach 410,000. Pancreatic cancer has strong characteristics of invasion, metastasis and recurrence, among whic...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/158C12Q2600/118
Inventor 陈长昊陈汝福李志花罗宇明郑汉豪赵月孔瑶安明杰
Owner SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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