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Application of gliquidone and cisplatin combined drug in preparation of antitumor drugs

An anti-tumor drug, the technology of gliquidone, which is applied in the field of medicine, can solve the problems that there are no reports on the application of anti-tumor drugs in combination with gliquidone and cisplatin

Active Publication Date: 2022-05-13
QIANFOSHAN HOSPITAL OF SHANDONG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The drug standard of gliquidone has been included in the second part of the Chinese Pharmacopoeia 2020 edition, and there is no report on the application of the combination of gliquidone and cisplatin in the preparation of anti-tumor drugs

Method used

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  • Application of gliquidone and cisplatin combined drug in preparation of antitumor drugs
  • Application of gliquidone and cisplatin combined drug in preparation of antitumor drugs
  • Application of gliquidone and cisplatin combined drug in preparation of antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 The proliferation inhibitory effect of gliquidone and cisplatin on A549 cells and A549 / DDP cells

[0018] cell source

[0019] A549 cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum, and passaged once every 2-3 days. The cell culture incubator is set at the following conditions: CO 2 Concentration: 5%, temperature: 37°C, cells in the logarithmic growth phase were used for the experiment.

[0020] A549 / DDP cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum + 1% P / S + 1 μg / mL CDDP, and passaged once every 2-3 days. The cell culture incubator is set at the following conditions: CO 2 Concentration: 5%, temperature: 37°C, cells in the logarithmic growth phase were used for the experiment.

[0021] Cell Viability Assay

[0022] The suspended A549 cells and A549 / CDDP cells were seeded in 96-well plates (6.5×10 3 each / well / 100μL), after incubation for 24h, add gliquidone (set concentration: 0.03, 0.1, 0.3, 1, 3, 10,...

Embodiment 2

[0042] Example 2 Gliquidone and CDDP drug resistance to A549 and A549 / CDDP cells

[0043] In order to further study the effect of gliquidone on the drug resistance of CDDP drug-resistant cells A549 / CDDP, the change of drug resistance index (RI) of CDDP drug-resistant cells A549 / CDDP before and after adding gliquidone (25μM) was detected by MTT method . It was found that in the experiment of A549 cells, before adding gliquidone, the measured IC 50 The value is 7.29μM, and after adding gliquidone, the IC of A549 cells 50 The value decreased to 4.00μM; while in A549 / CDDP cells, the IC of CDDP monotherapy 50 The value is 32.32μM, and it is 18.16μM when the drug is combined. By calculating the IC of two cells to different drugs 50 , the RI of A549 / CDDP cells to CDDP can be obtained, which is 4.43 times in the CDDP single drug group and 4.54 times in the combined drug group, and the reversal factor is 1.78 (Table 6). Therefore, the above data show that gliquidone can reverse th...

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Abstract

The invention provides an application of a gliquidone and cisplatin combined drug in preparation of an anti-tumor drug. The invention finds that when the gliquidone and the cisplatin are combined for use, the inhibition effect on lung cancer cells is obviously enhanced, the IC50 of the cisplatin in A549 and A549 / CDDP is 7.29 + / -1.11 mu M and 32.32 + / -0.17 mu M respectively, and the IC50 of the cisplatin and the gliquidone combined for use in A549 and A549 / CDDP is 4.00 + / -0.14 mu M and 18.16 + / -1.03 mu M respectively. The gliquidone can reverse the drug resistance of the drug-resistant cell strain A549 / CDDP to the CDDP to a certain extent.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of gliquidone and cisplatin in the preparation of antitumor drugs. Background technique [0002] Lung cancer is the most common malignant tumor and the leading cause of death. [0003] Cisplatin (CDDP) is the first-line chemotherapy drug after lung cancer surgery, but cisplatin has drug resistance, which limits the efficacy of cisplatin in lung cancer chemotherapy. Therefore, it is necessary to develop drugs that reverse cisplatin resistance in lung cancer. [0004] Gliquidone (YXM-11) is the second-generation oral sulfonylurea hypoglycemic drug, which is a highly active pro-islet β-cell agent. It binds to specific receptors on the islet β-cell membrane and can induce the production of an appropriate amount of insulin to reduce blood sugar levels. blood sugar concentration. The drug standard of gliquidone has been included in the second part of the Chinese Pha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/64A61K33/243A61P35/00
CPCA61K31/64A61K33/243A61P35/00A61K2300/00
Inventor 杨新美刘波李元媛赵维宋林林刘洪英
Owner QIANFOSHAN HOSPITAL OF SHANDONG
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