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Living sober small animal calcium imaging multifunctional detection platform under two-photon microscope

A photon microscope and detection platform technology, applied in the direction of using light for diagnosis, diagnostic recording/measurement, medical science, etc., can solve the problems of restriction, difficulty in the awake and free movement of mice, stimulation and other problems, and achieve high matching degree and equipment price. Inexpensive and stable effects

Pending Publication Date: 2022-06-03
AIR FORCE MEDICAL UNIV
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Problems solved by technology

The reason is that it is difficult to keep mice awake and free to move while two-photon recording is in progress, and it is impossible to give various conditioned stimuli at the same time.
[0003] The above reasons significantly restrict the study of two-photon calcium imaging of neural circuits when mice are kept awake and free to move, and given conditioned stimuli

Method used

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  • Living sober small animal calcium imaging multifunctional detection platform under two-photon microscope
  • Living sober small animal calcium imaging multifunctional detection platform under two-photon microscope
  • Living sober small animal calcium imaging multifunctional detection platform under two-photon microscope

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Embodiment Construction

[0041] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.

[0042] The embodiments of the present invention are implemented in this way, such as Figure 1 to Figure 8 The multifunctional detection platform for calcium imaging under two-photon microscopy in live awake small animals is shown, including:

[0043] Detection platform bracket 1;

[0044] The living body fixing bracket 2 is installed on the detection platform bracket 1 and is slidably connected with it, and is used for fixing the living body awake animal;

[0045] The living body detection piece is installed on the detection platform bracket 1 and is slidably connected with it, and is used for the movement of the living awake animal or the mechanical pain stimulation for the living awake animal or the cold and heat pain stimulation for the living awake animal accord...

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Abstract

The invention relates to a multifunctional detection platform for calcium imaging of small living sober animals under a two-photon microscope, and belongs to the technical field of calcium imaging of small animals under the two-photon microscope. The living body fixing bracket is mounted on the detection platform bracket, is in sliding connection with the detection platform bracket and is used for fixing a living body conscious animal; and the living body detection part is mounted on the detection platform bracket, is in sliding connection with the detection platform bracket, and is used for enabling a living sober animal to move or carrying out mechanical pain stimulation on the living sober animal or carrying out cold and hot pain stimulation on the living sober animal according to experimental requirements. According to the invention, the problem of giving various condition stimulation while recording the live sober mouse by the two-photon microscope is solved; mouse fixing and various condition stimulation platforms are integrated, the matching degree is high, and the stability is high; meanwhile, the system is suitable for observation and recording of two-photon real-time calcium imaging of the brain and spinal cord of a living body.

Description

technical field [0001] The invention relates to the technical field of calcium imaging of small animals under a two-photon microscope, in particular to a multifunctional detection platform for calcium imaging under a two-photon microscope of awake small animals in vivo. Background technique [0002] In recent years, with the development of technologies such as two-photon microscopy and calcium imaging, people have gained a deeper understanding of the structure and function of neural networks in mice. But so far, there have been few reports on the two-photon calcium imaging studies of neural circuits when mice are kept awake and freely moving and given conditioned stimulation. The reason is that it is difficult to keep the mouse awake and free to move while two-photon recording, and it is impossible to give various conditioned stimuli at the same time. [0003] The above reasons significantly restrict the two-photon calcium imaging study of neural circuits when mice are kept...

Claims

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Application Information

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IPC IPC(8): A61B5/00
CPCA61B5/483A61B5/4824A61B5/0059A61B2503/40A61B2503/42
Inventor 罗层褚文广解柔刚李腊梅郑星星
Owner AIR FORCE MEDICAL UNIV
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