Application of coptis chinensis detoxification decoction in preparation of sensitizer for tumor immune checkpoint blocking therapy

A technology of immune checkpoint and Huanglian Jiedu Decoction, which is applied in the direction of antineoplastic drugs, medical preparations containing active ingredients, and pharmaceutical formulas, to achieve the effects of increasing survival, increasing immune infiltration, and increasing secretion

Active Publication Date: 2022-07-22
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Claims
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AI Technical Summary

Problems solved by technology

[0004] Huanglian Jiedu Decoction is a classic traditional Chinese medicine for clearing away heat and detoxification. It has been reported in the past that it can sensitize tumors to radiotherapy and chemotherapy. At present, whether Huanglian Jiedu Decoction can sensitize tumor immune checkpoint blockade therapy has not been reported.

Method used

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  • Application of coptis chinensis detoxification decoction in preparation of sensitizer for tumor immune checkpoint blocking therapy
  • Application of coptis chinensis detoxification decoction in preparation of sensitizer for tumor immune checkpoint blocking therapy
  • Application of coptis chinensis detoxification decoction in preparation of sensitizer for tumor immune checkpoint blocking therapy

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Embodiment 1

[0039] 1. Establishment of a melanoma-bearing mouse model

[0040] B16F10 tumor cells in logarithmic growth phase were taken, resuspended in PBS after digestion and counted, and the cell concentration was adjusted to 1.5×10 7 / mL. 6-8 week old C57BL / 6J mice were shaved at the right posterior axillary line, and each mouse was inoculated subcutaneously with 1.5×10 6 / 100 μL.

[0041] 2. Grouping and administration of Huanglian Jiedu Decoction

[0042] Mice were divided into 4 groups with 6 mice in each group: control group, ICIs (anti-PD-1+anti-CTLA-4) group, Huanglian Jiedu Decoction (HLJD) group, and ICIs+HLJD combination group.

[0043] Control group: when the average tumor volume reaches 25-30mm 3 , Administer normal saline by gavage every day, 1 time / day, 400 μL each time, until the 16th day after tumor seeding; when the average tumor volume reaches 50mm 3 , given intraperitoneal injection of normal saline, once every 3 days, 200 μL per animal each time, 3 times in tot...

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Abstract

The invention provides application of coptis chinensis detoxification decoction in preparation of a sensitizer for tumor immune checkpoint blocking therapy, and relates to the field of traditional Chinese medicines. According to the application provided by the invention, the lifetime of the immune checkpoint blocking therapy to mice bearing melanoma can be remarkably prolonged; the tumor volume of a mouse bearing melanoma is reduced; the infiltration of immune cells in tumor tissues of melanoma-bearing mice is improved; a dendritic cell TLR7 / 8 pathway in mouse-bearing melanoma tissues is activated, and secretion of I-type interferon is increased.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to the application of Huanglian Jiedu Decoction in the preparation of a sensitizer for tumor immune checkpoint blockade therapy. Background technique [0002] Immune checkpoint blockade (ICB) is a hot spot in tumor immunotherapy in recent years. The basic principle of immune checkpoint blockade therapy is based on the activation mechanism of immune cells T cells. Programmed death receptors are expressed on the surface of T cells, and their ligands are expressed on the surface of tumor cells and myeloid-derived suppressor cells. The combination of programmed death receptors and their ligands can make T cells exhausted and unable to kill tumor cells normally, and tumor cells can escape the immune surveillance of the host. Therefore, programmed death receptors and their ligands are called "immune checkpoints". Immune checkpoint blockade therapy based ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/756A61K39/395A61P35/00
CPCA61K36/718A61K36/539A61K36/756A61K36/744A61K39/3955A61P35/00A61K2300/00Y02A50/30
Inventor 吴金峰刘苏青徐金华董竞成
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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