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Blood beta-hydroxybuyric acid electrochemical sensor

A oxybutyric acid and electrochemical technology, applied in the field of sensors, can solve the problems of not being able to use small clinic patients at home, measuring a large amount of blood, and taking a long time.

Inactive Publication Date: 2005-02-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the problem of this method is that it needs to use special equipment, the measurement requires a large amount of blood, takes a long time, and is expensive. It is only suitable for large hospitals and cannot be used in small clinics or patients' families.

Method used

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  • Blood beta-hydroxybuyric acid electrochemical sensor
  • Blood beta-hydroxybuyric acid electrochemical sensor
  • Blood beta-hydroxybuyric acid electrochemical sensor

Examples

Experimental program
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Embodiment Construction

[0020] Such as figure 1 and figure 2 As shown, the blood β-hydroxybutyric acid electrochemical sensor includes an insulating substrate 1 , and a working electrode 2 and a reference electrode 3 are covered on the substrate 1 . A working area 6 for blood sample measurement is set on the substrate 1 , and the working area 6 includes the working electrode 2 and the reference electrode 3 . The outside of the blood sample measurement working area 6 is covered with a chemically inert insulating covering film 5 . The working electrode 2 and the reference electrode 3 inside the blood sample measurement working area 6 are covered with an enzyme reagent reaction membrane 4 capable of reacting with β-hydroxybutyric acid and generating electron transfer. The enzyme reagent reaction membrane 4 is connected to the working electrode 2 and the reference electrode 3 respectively, and the working electrode 2 and the reference electrode 3 are respectively connected to the outside of the insula...

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Abstract

The method includes insulation base plate which is covered with working electrode and reference electrode as well as sets a blood speimen measuring operation region including both of working and reference electrodes. The external part of blood measurnig operation region is covered with insulation covering film of chemical inertia as the internal part of it is covered by enzyme reagent reation film which can be reacted with B-hydroxy-butanoic acid and to generate electron transmission. The enzyme reagent reaction film contains B-hydroxy-butanoic acid dehydrase, NAD, electron transmitting body, diaphorase, hydrophiblic high polymer and stabilizer for keeping activity of the enzyme.

Description

technical field [0001] The invention relates to a sensor for quantitatively detecting β-hydroxybutyric acid in blood samples, which is used for quantitatively and rapidly measuring blood ketone body levels. Background technique [0002] In recent years, more and more attention has been paid to measuring the concentration of β-hydroxybutyric acid in blood in diagnosis. At present, the detection method of β-hydroxybutyric acid is mainly carried out through a large-scale blood biochemical analyzer using a β-hydroxybutyric acid kit. However, the problem of this method is that special devices are required, the measurement requires a large amount of blood, takes a long time, and is expensive. It is only suitable for large hospitals and cannot be used in small clinics or patients' families. Contents of the invention [0003] The purpose of the present invention is to provide a sensor that can conveniently and quickly measure the conten...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/00G01N27/30G01N27/333G01N27/416G01N33/64
Inventor 李光管文军潘敏胡贵权马念章陈裕泉
Owner ZHEJIANG UNIV
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