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Enzyme detection reagent of uric acid for electrochemical test strip

A test strip, electrochemical technology, applied in the field of biosensing, can solve the problems affecting the accuracy of the detection results, imprecise sample quantification, prolonging the detection time, etc., to reduce errors, eliminate interference, and improve response performance.

Active Publication Date: 2015-04-08
复星诊断科技(长沙)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the blood sample is collected and the enzyme reaction is over, the results need to be interpreted with the help of optical instruments, which virtually prolongs the detection time and thus affects the accuracy of the detection results
In addition, blood samples are generally collected manually, and there are inaccurate problems in sample quantification, which will also affect the accuracy of test results

Method used

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  • Enzyme detection reagent of uric acid for electrochemical test strip
  • Enzyme detection reagent of uric acid for electrochemical test strip
  • Enzyme detection reagent of uric acid for electrochemical test strip

Examples

Experimental program
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Embodiment 1

[0024] see figure 1 , an electrochemical test strip using the enzyme detection reagent of the present invention, comprising an insulating substrate 1, printed with working electrodes 2, 3 and a T-type reference electrode 4 on the insulating substrate, and an insulating layer 5 covering part of the working electrodes and On part of the reference electrode, the reaction zone 6 formed by the enzyme layer is printed on the working electrodes 2, 3 and the reference electrode 4 by screen printing, and the double-sided adhesive tape is pasted on the edge of the reaction zone 6 to form an adhesive layer 7, and the upper part is coated with A hydrophilic membrane 8 is covered, and a micro-sampling cavity 9 is surrounded by an enzyme layer 6 , an adhesive layer 7 and the hydrophilic membrane 8 .

[0025] The thickness of the adhesive layer 7 is 0.1mm, and the distance between the two adhesive layers is 1mm; the volume of the sample injection cavity is not greater than 1.5uL.

[0026] T...

Embodiment 2

[0037] The enzyme detection reagent for uric acid used in electrochemical test strips contains 20 parts by weight of urate oxidase, 5 parts of potassium ferricyanide, 0.4 parts of polyethylene glycol, 5 parts of carboxymethyl cellulose, and the pH value is 60 parts of phosphate buffer solution of 5.0.

Embodiment 3

[0039] The enzyme detection reagent for uric acid used in electrochemical test strips contains 30 parts by weight of urate oxidase, 10 parts of potassium ferricyanide, 1 part of polyethylene glycol, 6 parts of carboxymethyl cellulose, and the pH value is 70 parts of phosphate buffer solution of 7.0.

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Abstract

The invention belongs to the technical field of biosensing, and relates to an enzyme detection reagent for detecting uric acid content for an electrochemical test strip. The enzyme detection reagent disclosed by the invention comprises the following components in parts by weight: 20-30 parts of urate oxidase, 5-10 parts of an electronic media body, 5.4-9 parts of a binder, and 60-70 parts of pH buffer solution. The test strip of the enzyme detection reagent disclosed by the invention has any known structure in the technical field. The enzyme detection reagent disclosed by the invention can improve the detection accuracy, is matched with a reagent strip with an optimized structure and corresponding data treatment method, and can detect the content of uric acid by a little of blood within short time.

Description

technical field [0001] The invention belongs to the technical field of biosensing, and relates to a detection reagent for electrochemical test strips, in particular to a detection reagent for uric acid used in electrochemical test strips. Background technique [0002] Uric acid is the final product of purine metabolism in the human body and has no physiological function in the human body. Under normal circumstances, it is mainly excreted by the kidneys. Too high or too low uric acid concentration can cause uric acid syndromes such as polycythemia vera, hyperlipidemia, acute and chronic nephritis, renal tuberculosis, malignant blood diseases, gout, chronic leukemia, and multiple myeloma. Detecting the concentration of uric acid in serum can help diagnose tumors and leukemia, and can be used as indicators of gout, rheumatoid arthritis, nephritis and other diseases in clinical diagnosis, and is more helpful for early diagnosis of kidney lesions. [0003] The methods for detec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26
Inventor 彭勇李文韬
Owner 复星诊断科技(长沙)有限公司
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