Method for increasing hyperosmotic-resistant yeast-fermented glycerol concentration by adding amphotericin fermented broth

A technology resistant to hypertonic yeast and amphotericin, applied in fermentation and other directions, can solve the problems of high price and increased production cost, and achieve the effect of low cost, easy control and increased concentration

Inactive Publication Date: 2005-12-07
TSINGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high price of amphotericin pharmaceutical grade reagents, the use in industrial production will increase production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Put 50ml of glycerin fermentation medium in a conical flask, connect with 5ml of hypertonic yeast strain, ferment at 35°C and shaker speed of 160r / min for 24 hours, add 0.25ml of amphotericin fermentation broth to continue Fermented and cultured to 36 hours, compared with the concentration of fermented glycerol in the fermented broth without adding amphotericin, the concentration of fermented glycerol in the fermented broth with amphotericin was increased by 41%.

Embodiment 2

[0016] Put 50ml of glycerin fermentation medium in a conical flask, connect 5ml of hypertonic yeast strain, ferment at 34°C and shaker speed of 160r / min for 24 hours, add 0.5ml of amphotericin fermentation broth to continue Fermented and cultured to 72 hours, compared with the concentration of fermented glycerol in the fermented broth without adding amphotericin, the concentration of fermented glycerol in the fermented broth with amphotericin was increased by 43%.

Embodiment 3

[0018] Put 50ml of glycerin fermentation medium in a conical flask, connect with 5ml of hypertonic yeast strain, ferment at 33°C and shaker speed of 160r / min for 24 hours, add 1ml of amphotericin fermentation broth to continue fermentation After culturing for 36 hours, compared with the concentration of fermented glycerol in the fermented broth without adding amphotericin, the fermented glycerol concentration in the amphotericin-added fermented broth increased by 34%.

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Abstract

The invention discloses a method for enhancing osmophilic yeast yeasting glycerine density by adding in amphiprotic zymoid yeasting liquid in glycerine producing field. The invention inoculates somphilic yeast strain in glycerine yeasting incubation media into glycerine yeasting incubation media, at the same time, in the process of glycerine yeasting, adds in amphiprotic zymoid yeasting liquid to carry on glycerine yeasting. The added amphiprotic zymoid yeasting liquid improves the membrane permeability of osmophilic yeast cell, and adjusts the permeability pressure, makes the glycerine in cell secreted out of the cell, thus enhances the density of glycerine to 10-90%. The process is simple, easy to be controlled, low cost, easy to be used in industry.

Description

technical field [0001] The invention belongs to the technical field of glycerin preparation. It particularly relates to a method for increasing the concentration of fermented glycerin by adding amphotericin fermentation liquid in the process of producing glycerin by hyperosmotic yeast fermentation. Background technique [0002] The production of glycerin by fermentation originated during World War I. At that time, Germany needed a large amount of glycerin to make explosives. It first invented the process of adding sulfite to yeast fermentation mash to produce glycerin, and built a factory for mass production. Due to the unreasonable process, serious environmental pollution, low production level, and high production cost, the production could not continue, and it stopped after the war. Afterwards, many countries, especially developed countries, attached great importance to the research of fermented glycerol, and carried out a lot of experimental research work. However, due t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/20
Inventor 张建安杨燕鸥刘德华谢东明杨海荣
Owner TSINGHUA UNIV
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