Animal brain extract and bitter gourd extract composition and its uses

A technology of bitter gourd extract and animal brain, which can be used in drug combinations, medical preparations containing active ingredients, metabolic diseases, etc., and can solve problems such as single action

Inactive Publication Date: 2004-08-25
邵园园
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] One of the technical problems to be solved in the present invention is to provide a medicine, health care product and food formulated with neurotrophic factors and gangliosides as the main components of brai...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: the extraction of neurotrophic factor

[0049] 3000g of clean animal brain was soaked in Hank solution, crushed, and centrifuged at 2000r / min for 20min. The precipitate was washed twice with Hank solution, resuspended with 2.5 mol / l sucrose in PBS, and the resulting mixture was extracted with 1.0 mol / l and 2.5 mol / l sucrose in PBS. After centrifugation at 1500r / min, the material between the upper layer and the middle layer was collected. Soak in 0.5 mol / l PBS (plus 5% cholic acid), after homogenization, centrifuge at 15,000 r / min for 45 min at 4°C, pass the supernatant over a GST column, and elute with 2 μg / ml glutathione washing solution. 600 mg of brain-derived neurotrophic factor was collected. The resulting neurotrophic factor is encapsulated with cyclodextrin.

Embodiment 2

[0050] Embodiment 2: the preparation of invention

[0051] Mix 1 part of brain extract, 12.5 parts of bitter melon extract, and 5 parts of starch, and pack them into capsules.

Embodiment 3

[0052] Embodiment 3: the effect of the present invention on blood sugar, nerve function and memory function of diabetic patients

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PUM

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Abstract

The present invention discloses a medicine composed of neural active substance extracted from animal brain and bitter melon extract, its health-care product and food with the functions of delaying senility, improving memory and curing and preventing diabetes and its complication are also disclosed. Its preparation process includes the following steps: soaking clean animal brain with Hank liquor, crushing, centrifugalizing, precipitating, washing twice by Hank liquor, using cane sugar and PBS to make resuspension, extracting, centrifugalizing, collecting the substance between upper layer and intermediate layer, soaking the obtained material with PBS in which 5% cholalic acid is added, homogenizing, centrifugalizing at 4 deg.C, making supernatant fluid pass through GST column, eluting with glutathione eluent to obtain neural active substance using brain source neurotrophic factor as main component, covering it with CD, then combining the animal brain extract with bitter melon extract according to a certain ratio so as to obtain said product.

Description

technical field [0001] The invention relates to a brain-extracted neuroactive substance for treating nervous system diseases, in particular to compound medicine, health care product and food of neurotrophic factor (BDNF), ganglioside (Ganglioside), and bitter melon extract. Background technique [0002] Gangliosides are found mainly in the gray matter of mammals and are characteristic components of neuronal membranes. At present, it is believed that the changes of ganglioside substances on the cell membrane are of great significance to the regulation of neurons and the transmission of information inside and outside cells. Studies have shown that exogenous gangliosides can increase the function of nerve growth factors after binding to cell membranes, promote nerve growth, and accelerate the recovery of nerve function. A trophic effect that increases axonal growth of sensory, sympathetic, and central cholinergic neurons. Animal experiments have shown that gangliosides have a...

Claims

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Application Information

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IPC IPC(8): A61K38/02A61P3/10A61P43/00
Inventor 邵园园陈良
Owner 邵园园
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