Process for prepn. of polyhydroxy alkanoate by using maoC gene

A chain polyhydroxyalkanoate and gene technology, applied in the field of preparing PHA, can solve problems such as no results
CN1539977AInactive Publication Date: 2004-10-27KOREA ADVANCED INST OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
KOREA ADVANCED INST OF SCI & TECH
Publication Date
2004-10-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to a method for producing middle-chain-length polyhydroxyalkanoate (MCL-PHA) using a maoC gene. The producing method of MCL-PHA according to the present invention comprises the steps of transforming a microorganism with the maoC gene to give a transformant, the microorganism being deleted of a fadB gene and containing a PHA synthase gene; culturing the transformant in medium containing a C6-10 carbon source; and obtaining PHA consisting of monomers with 6-10 carbon atoms. When the maoC gene whose function has not yet been established is used according to the present invention, high quality PHA with a higher number of carbon atoms than the prior PHA can be produced at a higher efficiency.
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Description

technical field

[0001] The present invention relates to a method for preparing polyhydroxyalkanoate (PHA described below) using maoC gene. More specifically, the present invention relates to a maoC gene, a protein having enoyl-CoA hydratase activity, and a method for producing PHA using a recombinant vector containing the maoC gene and a microorganism transformed with a recombinant vector containing a PHA synthesis gene method. Background technique

[0002] PHA is a polyester-type compound that accumulates in microorganisms to serve as a carbon source when excess carbon exists in microorganisms and lacks other nutrients such as phosphorus, nitrogen, magnesium, and oxygen. Since PHA has similar physical properties to gasoline-derived synthetic polymers and is completely biodegradable, it is considered as a substitute for synthetic plastics that were previously used.

[0003] Generally, PHAs are classified into short-chain PHAs containing several carbon atoms (SCL-PHA) and m...

Claims

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