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Method for mutagenizing anti-chlorosulfuron somatic mutant of rice

A technology of chlorsulfuron and mutants, applied in plant cells, horticultural methods, botanical equipment and methods, etc., can solve the problems of restricting the use of chlorsulfuron herbicide, long research cycle, huge investment, etc., and save manpower , better effect and wide adaptability

Inactive Publication Date: 2005-02-23
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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Problems solved by technology

[0002] With the rapid development of agricultural herbicides, the safety of herbicide use has become increasingly prominent, which has attracted widespread attention. Starting from the improvement of herbicides, the continuous introduction of new varieties of high-efficiency, low-toxicity, and low-residue herbicides is a good way to solve the problem. , but requires huge investment, and its research cycle is long; in addition, rice is extremely sensitive to chlorsulfuron herbicide, and the content of chlorsulfuron in soil is 0.1-0.2×10 -9 When the growth of rice plants is inhibited, the plants will become stiff and the roots will be in the shape of chicken feet; the wheat fields treated with chlorsulfuron are not suitable for rice seedling fields, direct seeding fields and small seedling transplanting fields, so the application of chlorsulfuron-methyl herbicide in the Yangtze River is seriously restricted. Use of downstream wheat and rice rotation areas

Method used

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Embodiment Construction

[0012] 1. Preparation of culture medium. Induction medium: 2mg / L dichlorophenoxyacetic acid (2,4-D) and 20-30mg / L 5-bromopyrimidine (5-Bu) were added to the basic medium (NB); Add 2mg / L2, 4-D and 0.2mg / L6-benzylaminopurine (6-BA); add 2mg / L2, 4-D and 0.2mg / L6-BA and 25-50mg to the screening medium (NB) / L chlorsulfuron; add 2mg / L6-BA and 0.4-0.5mg / L naphthaleneacetic acid (NAA) in the differentiation medium (MS); add 2mg / L6-BA and 0.5mg / L in the differentiation medium (MS) LNAA and 50-60mg / L chlorsulfuron.

[0013] 2. Induction, subculture, and screening culture time: the inoculated mature embryos and the transferred callus screening materials are placed in a biochemical incubator, and the induced and screening callus must be cultivated in the dark. The culture temperature is controlled at 26- 27 ℃, otherwise, the growth quality of the callus induced is poor; (1) induction culture: the mature seeds are shelled, and the 2 ) solution for 15 minutes, rinsed with sterile water ...

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Abstract

A method for inducing the chlorsulfuron-resistant somatic mutant of paddy rice includes such steps as removing husk from the health seeds, immersing in alcohol and then in mercury chloride solution, flushing by aseptic water, inducing culture, secondary culturing, screening culturing by adding chlorsulfuron, differential culturing and redifferential culturing.

Description

technical field [0001] The present invention relates to a method for improving the breeding efficiency of rice herbicide resistance, and more specifically relates to a method for inducing a chlorsulfuron-resistant somatic mutant in rice. A practical approach to plant cell engineering for the herbicide novel variety pathway. Background technique [0002] With the rapid development of agricultural herbicides, the safety of herbicide use has become increasingly prominent, which has attracted widespread attention. Starting from the improvement of herbicides, the continuous introduction of new varieties of high-efficiency, low-toxicity, and low-residue herbicides is a good way to solve the problem. , but requires huge investment, and its research cycle is long; in addition, rice is extremely sensitive to chlorsulfuron herbicide, and the content of chlorsulfuron in soil is 0.1-0.2×10 -9 When the growth of rice plants is inhibited, the plants will b...

Claims

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Application Information

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IPC IPC(8): A01H3/04A01H4/00C12N5/04
Inventor 冯双华肖国樱
Owner INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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