Absorbent article comprising a yucca extract
An absorbent, product technology, applied in absorbent pads, medical science, bandages, etc., can solve the problems of incontinence adult embarrassment, ammonia embarrassment, diaper rash, etc.
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Embodiment 1
[0069] Various commercially available yucca extracts used as animal feed supplements were tested to determine their urinary enzyme inhibitory efficacy. 2 milliliters (ml) of each yucca extract, 18 ml urine, and 2 ml canavalan urease (10 milligrams / milliliter (mg / ml)) were placed in a 50 ml conical tube. Concanavalin urease was obtained from Sigma Chemical Company of St. Louis, Missouri as U-4002. The final concentration of urease used was 0.91 mg / ml (1.0 mg urease = 1000 U). The conical tube was capped and the contents mixed vigorously with a vortexer. After incubation at 25°C for 2 hours, the upper gas phase of the mixture was analyzed for ammonia using a Gastec(R) ammonia test tube obtained from Gastec Corporation of Ayase-shi Kanagawa-ken, Japan. A control mixture containing 2 ml of deionized water, 18 ml of urine and 2 ml of canavalan urease was also analyzed.
[0070] The four yucca extracts tested included: 100% pure Mojave Yucca Powder, sold by Sher-Mar Enterprises i...
Embodiment 2
[0073] Various concentrations of Mojave yucca extracts were studied to determine their urinary enzyme inhibitory efficacy. A certain amount of Mojave Yucca solution (sold by Sher-Mar Enterprises under the trade name Yucca 70) was thoroughly mixed with 18 ml of urine and 2 ml of canavalan urease in a vortexer. After incubation for 2 hours at 25°C, the resulting mixture was analyzed using ammonia assay tubes. A control mixture containing only 18 ml of urine, 2 ml of deionized water and 2 ml of canavalan urease was also analyzed.
[0074] The results for the mixture containing yucca extract were compared to the control mixture. The final concentrations of yucca extract analyzed were 0 wt% (control mixture), 0.9 wt%, 4.6 wt% and 9.1 wt% per volume. The 0.9% mixture contained 6.3 mg of yucca extract solids per mL of urine, which demonstrated an approximately 54% reduction in gas phase ammonia compared to the control mixture. The 4.6% mixture, which contained 31.5 mg yucca extrac...
Embodiment 3
[0077] This example is similar to Example 2, except Proteus mirabilis was used instead of concanavalin urease. Mojave yucca extracts at various concentrations were studied to determine their inhibitory efficacy against these urinary enzymes.
[0078] In 10 ml of Trypticase Soy Broth (TSB) (available from Difco in Ann Arbor, Michigan) in 15 ml sterile, loosely screw-on capped conical tube at 37°C Proteus mirabilis (ATCC 29906) bacteria were recovered from the frozen state by growing overnight MicroBank Beads (available from Pro Lab, Inc., Austin, Texas) coated with the appropriate bacteria. Hold the conical tube still. The purity of the bacterial suspension was checked by isolation plates and Gram staining based on the observation of turbidity. Once the isolate was determined to be Proteus mirabilis, colonies were transferred from the isolation plate into 10 ml TSB in a sterile 15 ml conical screw cap tube and grown overnight at 37°C under facultative conditions. The bacteri...
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