Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tumor therapeutic agent and usage thereof

A technology for tumor treatment and therapeutic agents, applied in gene therapy, antineoplastic drugs, genetic material components, etc., can solve problems such as unsatisfactory specificity, affecting treatment effect, and few effective gene types

Inactive Publication Date: 2005-09-21
王健伟 +2
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current problem of tumor gene therapy is that there are few types of effective genes and the specificity is still not ideal, which seriously affects the therapeutic effect. Difficulties and Hotspots of Gene Therapy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tumor therapeutic agent and usage thereof
  • Tumor therapeutic agent and usage thereof
  • Tumor therapeutic agent and usage thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The content of this example uses adenovirus as a carrier to control the expression of HIV Vpr gene by using the alpha-fetoprotein-hypoxia response element promoter, and proves that the preparation has a specific killing effect on tumor cells and can enhance chemotherapy effect. Specific steps are as follows:

[0037] Unless otherwise specified, all molecular cloning operations below are the same as those written by J. Sambrook and D.W. Russell.

[0038] 1. Construction of adenovirus shuttle plasmid pShuttlHREAFPVpr

[0039] (1) Construction of adenovirus shuttle plasmid pShuttle-hcEgr-CMS-Poly(A)

[0040] Plasmid BSVprThy (pVPR for short) containing HIV vpr gene, see Jowett JBM, PlanellesV, Poon B, Shan NP, Hen ML, Chen ISY. The Human Immunodeficiency Virus Type 1 vprGene Arrests Infected T Cells in the G2+M Phase of the Cell Cycle .J Virol, 1995,69:6304-13); pCI-neo plasmid was purchased from Promega, pCDNA II plasmid was purchased from Invitrogen Company, adenoviru...

Embodiment 2

[0150] Unless otherwise indicated, the specific experimental operations in this example are the same as those in Example 1.

[0151] Studies have shown that radiation therapy can arrest tumor cells in G2 phase, thereby inhibiting tumor growth. The content of this example is to use the radiation promoter (Egr-1) in the adenovirus vector to control the expression of the HIV Vpr gene, which proves that the preparation can specifically enhance the cell cycle G2 arrest effect on tumor cells after radiation induction, so that it can Enhance the effect of tumor radiation therapy. Specific steps are as follows:

[0152] 1. Construction of recombinant adenovirus shuttle plasmid pShuttleEgrVpr

[0153] (1) Digest pE425 containing a ray-responsive promoter (Egr-1) with Sal I (5' end) and BamH I (3' end), (see Seung LP, Mauceri HJ, Beckett MA, Hallahan DE, HellmanS, Weichselbaum RR. Genetic radiotherapy overcomes tumor resistance to cytotoxic agents. Cancer Res, 1995, 55:5561-5.), obta...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the tumour genes treatment, as more specific, it relates to a new tumour therapeutic agent and its usage. The tumour therapeutic agent contains human immunodeficiency virus Vpr gene, which is in control of a promoter and express specificly in the tumor structure. The invention can be used in tumor treatment, and can be the intensifier in tumor chemical therapy or radiation therapy.

Description

technical field [0001] The present invention relates to gene therapy for tumors. More specifically, the present invention relates to a novel tumor therapeutic agent and its use. Background technique [0002] Tumor is one of the most important diseases that endanger human health at present, and the incidence rate is constantly increasing, and it has become one of the most important causes of death for human beings. According to reports, in 1997, the expected number of deaths from cancer in the United States was 560,000, and the number of new cases was more than 1.38 million, accounting for the second place among all causes of death. From 1990 to 1992, the results of a sample survey in 27 provinces, municipalities, and autonomous regions across the country showed that malignant tumors ranked second in the cause of death in my country, second only to respiratory diseases, and the crude death rate of tumors was 108.26 / 100,000, accounting for Consists of 17.94%. Recently, exper...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K48/00A61P35/00
Inventor 王健伟魏强洪涛
Owner 王健伟
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com