Metallothionein-like protein gene of Tamarix androssowii Litw.

A technology of metallothionein and tamarisk, applied in the field of genetic engineering, can solve the problems of lack of genes, strong stress resistance of salt-resistance-related genes, limited types and numbers of genes of stress-resistant plants, etc.

Inactive Publication Date: 2006-09-06
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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Problems solved by technology

[0004] At present, in genetic engineering technology, the types and quantities of genes from stress-resistant plants are very limited, and there is a lack of genes cloned from high-stress-resistant woody plants; since most stress-resistant genes are cloned from non-stress-resistant plants, such as pseudo Obtained from plants such as Arabidopsis thaliana and rice (Oryza sativa), the salt-resistance-related genes of non-stress-resistant plants obviously cannot have strong stress resistance; moreover, most of the stress-resistance-related genes come from herbaceous plants, microbes, etc., due to genetic differences between them and woody plants, these genes may not be very effective in woody plants

Method used

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  • Metallothionein-like protein gene of Tamarix androssowii Litw.

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Embodiment

[0022] By constructing a suppressive subtractive cDNA library, then randomly sequencing the library clones, and performing BlastX analysis on the sequencing results, the specific steps are:

[0023](1) Construction of a suppressive subtractive library. 2 μg of mRNA from the drought treatment and control materials were taken respectively, and 5'TTTTGTACAAGCTT30N 3' was used as a primer to synthesize the first and second strands of cDNA using the cDNA synthesis Kit (Bao Bioengineering (Dalian) Co., Ltd.). The synthesized double-stranded cDNA was digested with RsaI, and the digested products were purified and precipitated, dissolved in 5.5 μL of ultrapure water, and 0.5 μL of each was used for agarose gel electrophoresis detection. The drought-treated cDNA was used as a tester, and the control cDNA was used as a driver. Take 1 μL of the tester cDNA and add 5 μL of water to dilute, take 2 μL of the diluted solution to connect with Adaptor1 and Adaptor2 respectively, the connectio...

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Abstract

Get the 3í»cDNA fragment by decreasing treasury, then get the cDNA sequence by cloning with RACE technology, the length of 3 non code area is 262bp, the length of 5 non code area is 150bp, the length of code area is 210bp, containing 69 amino acids, and the molecular weight of code protein is 7.32KD. The metallothionin gene is against heavy metal, drought and salt. The metallothionin is non-enzymic protein which has low molecular mass and high mercapto groups and metals. The said gene is the resistant gene to improve the resistance ability of plant.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to the cloning of a cDNA sequence of a metallothionein-like protein gene of Tamarix candidum; the metallothionein-like protein gene belongs to a class of genes in the metallothionein family and is a This species has genes for resistance to heavy metal stress, drought resistance, and salt tolerance. Therefore, the gene of the present invention is a kind of stress resistance gene, and its specific application is to improve the stress resistance ability of plants through genetic modification. Background technique [0002] At present, the application of transgenic technology to improve plant resistance has become a consensus, and there have been reports of various genes being patented. Tamarix albicans is extremely resistant to drought and salt. The reason is that there are a series of genes with strong stress resistance in its body. Therefore, Tamarix ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415
Inventor 张道远王玉成董玉芝
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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