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PCR detection reagent kit for sexual disease mixed infection

A detection kit and mixed infection technology, applied in the field of biomedicine, can solve problems such as screening of unfavorable mixed infections and STDs

Inactive Publication Date: 2006-11-22
广州中医药大学热带医学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the PGR detection kits developed in China are all one kit for one disease, which is not conducive to the screening of mixed sexually transmitted diseases

Method used

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  • PCR detection reagent kit for sexual disease mixed infection
  • PCR detection reagent kit for sexual disease mixed infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: the design of primer sequence

[0041] 1) Hepatitis B virus: S(HBsAg) gene (155bp-835bp) and its upstream and downstream partial sequences: 56bp-841bp, fragment length 786bp.

[0042] Upstream primer: 5'-CCTGCTGGTGGCTCCAGTTCCGGA-3'

[0043] Downstream primer: 5'-TAGGGTTTAAATGTATACCCAAAG-3'

[0044] 2) Human immunodeficiency virus (human immunodeficiency virus type 1): gag gene (790bp-2295bp): 1216bp-1841bp, fragment length 626bp.

[0045] Upstream primer: 5'-AGAGGATCCAGGGGCAAATGGTA-3'

[0046] Downstream primer: 5'-TCCCGGTACCTGTCATGCTGTCA-3'

[0047] 3) Candida albicans (Candida albicans): autonomously replicating sequence: 27bp-710bp, fragment length 684bp.

[0048] Upstream primer: 5'-TAGCGATGAGGTAGTGCAAG-3'

[0049] Downstream primer: 5'-GCTGCAGCTACGATTGTTAG-3'

[0050] 4) Ureaplasma urealyticum: urease complex ureB gene (495-869bp) and ureC gene (908bp-2704bp): 521bp-981bp, fragment length 461bp.

[0051] Upstream primer: 5'-TCCAGGTAAATTAGTACCAGG-...

Embodiment 2

[0071] Example 2: Component preparation of amplification tubes

[0072] The total volume of the amplification reaction mixture is 23 μL, and its composition is shown in Table 1.

Embodiment 3

[0073] Example 3: Sample pretreatment and gene amplification

[0074] Stir the cotton swab in 500μl normal saline, then centrifuge at 12000r / min for 3min, discard the supernatant, add 50μl pretreatment solution to the pellet, mix well, boil for 10min, centrifuge at 12000r / min for 5min, take 2μL of the supernatant and add it to the amplification medium. In the amplification reaction tube, start the amplification reaction. The reaction conditions are as follows: denaturation at 93°C for 3 minutes; 35 cycles of 93°C for 45 seconds, 55°C for 45 seconds, and 72°C for 1 minute; elongation at 72°C for 5 minutes.

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Abstract

The invention discloses a synchronizing detection PCR agent box (hepatitis B virus, human immunodeficiency virus, human breast tumor virus, lues helicoid, human cytomegalovirus, simple herpesvirus, white oidiomycin, urea-dissolving mycoplasma, gonotoxin and Chlamydi trachomatis), which consists of blood type and secretion stains type. The user adds predisposed sample in the enlarging pipe to start enlarging reaction, which finishes detecting work simply and rapidly. The invention can detect ten venereal disease causal agents, which is fit for customs quarantine, prevailing monitor and hospital screen examination.

Description

technical field [0001] The invention relates to biomedical technology, in particular to a polymerase chain reaction kit for synchronous detection of mixed infection of venereal disease, referred to as a PCR detection kit for mixed infection of venereal disease. Background technique [0002] Sexually transmitted diseases (STDs) are a group of diseases that are spread primarily through sexual contact between an infected and an uninfected person. STDs have become one of the fastest-rising infectious diseases in my country. The incidence of STDs, the types of STD pathogens discovered, and problems related to other infectious diseases are increasing. The frequent occurrence of STDs has also significantly accelerated the spread of AIDS. The currently discovered sexually transmitted pathogens have expanded from the original 4 species to more than 20 species today, including 7 categories including bacteria, fungi, viruses, chlamydia, mycoplasma, spirochetes, and parasites. It is es...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 曾庆平冯丽玲杨瑞仪符林春
Owner 广州中医药大学热带医学研究所
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