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Use of anticholinergic M3-type receptor antibody determination for diagonsing sicca syndrome

A cholinergic and receptor technology, applied in the field of anticholinergic M3 receptor antibody determination in the diagnosis of Sjogren's syndrome, can solve problems such as poor medical compliance, delayed diagnosis, and lost treatment opportunities

Inactive Publication Date: 2007-01-17
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no ideal serological diagnosis method has been found clinically. In addition to clinical symptoms, the diagnosis is mainly made by invasive examinations such as lip gland biopsy and parotid angiography. Due to poor medical compliance of patients, diagnosis is often delayed and the best treatment is lost. opportunity

Method used

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  • Use of anticholinergic M3-type receptor antibody determination for diagonsing sicca syndrome
  • Use of anticholinergic M3-type receptor antibody determination for diagonsing sicca syndrome
  • Use of anticholinergic M3-type receptor antibody determination for diagonsing sicca syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Identification of cholinergic M3 receptors

[0021] Cholinergic M3 receptor is a cholinergic receptor mainly distributed in exocrine glands and smooth muscle, and it is also a G-protein coupled receptor. Recent studies have found that anticholinergic M3 receptor antibodies in serum of SS patients can recognize and activate M3 receptors. In the past, it was believed that the epitope of the cholinergic M3 receptor was located in the second extracellular loop. However, it was found that the second extracellular loop polypeptide of the M3 receptor was used to detect the anti-cholinergic M3 receptor antibody in the serum of SS patients. The reproducibility of the method is not good [8]. Some studies have suggested that cholinergic M3 receptors may be conformational epitopes [28], thus suggesting that anticholinergic M3 receptor antibodies probably recognize the spatial structure of M3 receptor proteins. Therefore, in this study, eukaryotically expressed recombina...

Embodiment 2

[0026] Example 2 Detection of Anticholinergic M3 Receptor Antibodies in the Serum of Sjögren's Syndrome Patients and Control Groups

[0027] The immunoblotting method established in this study (Western Blot) was used to detect the anticholinergic M3 receptors in the serum of patients with Sjogren's syndrome (SS), rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and normal controls. antibody to evaluate the distribution of the antibody in various diseases.

[0028]Firstly, the anticholinergic M3 type receptors were subjected to protein SDS-PAGE, and then transferred to nitrocellulose membranes and blocked with skim milk overnight. Incubate it with the serum to be tested. If there is anti-cholinergic M3 receptor antibody in the serum, the antibody will bind to the antigen on the nitrocellulose membrane to form an antigen-antibody complex. After washing unbound antibodies and other components, incubate with biotin-labeled goat anti-human IgG. After washing away unbo...

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Abstract

Determination of anticholinergic M3 receptor and antibody is used to diagnose sicca syndrome. It has excellent sensitivity and specificity.

Description

[0001] The invention relates to the application of the anti-cholinergic M3 receptor antibody as an antigen in detecting Sjögren's syndrome and other diseases. 1. Technical background [0002] Sjogren's syndrome (SS) is a systemic autoimmune disease characterized by invading exocrine glands such as lacrimal glands and salivary glands. The most common clinical manifestations of the disease are dry eyes and dry mouth, and can involve the lungs, kidneys, lymphatic system, blood vessels and liver and other organs. The disease has no obvious regional distribution difference, and can occur at any age, with 40-60 years old, especially postmenopausal women in the majority, and the ratio of male to female is 1:9. The prevalence rate in my country is 0.77%, and the prevalence rate in the elderly population is as high as 4%. There are nearly 10 million patients in the whole country. [0003] In recent years, people have conducted a lot of research on the etiology and pathogenesis of Sjog...

Claims

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Application Information

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IPC IPC(8): C07K14/705G01N33/558G01N33/53
Inventor 栗占国
Owner PEOPLES HOSPITAL PEKING UNIV
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