Production of 2-furfurylthiol in brassica seed and use of same
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[0028] These examples are provided to help illustrate various aspects of the invention. However, the examples do not limit the scope of the invention.
[0029] All of the seeds used in these working examples were obtained from Forbes Frozen Foods of Cincinnati, Ohio.
[0030] 1. Concentration of FFT in Heated Brassica Seed
[0031] Approximately ten grams each of various types of Brassica seed were heated as described in Table 1 below. The concentration of FFT subsequently was determined by using a stable isotope dilution assay, although other procedures may be used, as known to those of ordinary skill.
[0032] The heated Brassica seed was weighed and extracted with dichloromethane (50 ml) containing deuterated FFT ([.sup.2H.sub.2]-furfur-ylthiol, at a concentration of 20 .mu.g per 50 mg). The volatile compounds subsequently were isolated by applying the high vacuum transfer technique described by Jung et al. (Lebensm.-Wiss. U Technol. 225:55-60, 1992), the Jung et al. article being incorporat...
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