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Device for containing, reacting and measuring, and method of containing, reacting and measuring

a technology of reacting and measuring devices, applied in the field of devices for reacting and measuring, and containing, can solve the problems of easy error in determining the luminescence position, the probability of cross-contamination occurring, and the amount of oligonucleotides at each fixed position becomes even smaller, so as to achieve accurate measurement and promote reaction

Inactive Publication Date: 2005-06-30
PSS BIO INSTR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"The invention is an integrated carrier that has a base member with various substances for detection fixed at specific positions along its length. The base member is rolled up onto a carrier, with each position exposed on the surface. This makes it easy to measure or detect the labels associated with each position. The carrier can be placed in a container section with a liquid inlet / outlet, allowing for reaction and measurement between the substance for detection and the target substance in the liquid. The container section has a transparent window so that the contents can be easily identified. The invention simplifies handling and allows for consistent processing."

Problems solved by technology

However, in order to manufacture the DNA chip, with the arrangement of a large number of different oligonucleotides at a high density in a plane on a restricted region, not only is there the likelihood of cross-contamination occurring due to these becoming close to each other, but also the oligonucleotides at each of the fixed positions becomes an even smaller amount.
In particular, if the oligonucleotides at each of the fixed positions becomes a small amount, determining the luminescence position is susceptible to error so that there is a problem with accuracy.
Furthermore, due to miniaturization, there is a problem in that the encounter characteristics or reactivity of the target substance is reduced, so that processing takes time.
Moreover, since the sample is arranged in a plane, then with higher densities, the handling and automation thereof is even more difficult.
Consequently, the manufacture of the DNA chip requires a considerable amount of effort and time, resulting in high cost.
However, even though the manufacture of such an integrated carrier is simplified and the cost reduced, there is a problem in that if reaction, measuring and identification using such an integrated carrier cannot also be performed efficiently and quickly, the advantage of this integrated carrier cannot be sufficiently realized.

Method used

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  • Device for containing, reacting and measuring, and method of containing, reacting and measuring

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first embodiment

[0052]FIG. 1(a) schematically shows a device for containing, reacting and measuring 10 according to a

[0053] The device for containing, reacting and measuring 10 according to this embodiment has a transparent slender tube 11 serving as the container section and having a liquid inlet 12, a pump 13 connected to the slender tube 11 and serving as a drawing and discharging section for drawing and discharging liquid into and from the slender tube 11, and a measuring device 14. Inside the slender tube 11 it is possible to contain a liquid, and a base member 15 immersed in the liquid.

[0054] The base member 15 is formed in a long and slender shape, and detection substances such as for example oligonucleotides having already known various base sequences are arranged so as to be lined up along the longitudinal direction thereof. The base member 15 is contained inside the slender tube 11 in an extended and secured condition so as to adhere to the slender tube 11. Here, reference symbol 16 show...

second embodiment

[0058] Next, FIG. 2 shows a device for containing, reacting and measuring according to a

[0059]FIG. 2(a) shows a core 21 serving as a rod shape or cylindrical shape carrier for carrying the aforementioned base member 15 rolled around the surface thereof. FIG. 2(b) shows an integrated carrier 22 with the beforementioned base member 15 rolled. Here the diameter of the core 21 is for example from approximately 2 to 4 mm, while the thickness of the base member 15 is from approximately 0.05 mm to 0.2 mm, and the length of the base member 15 is for example from approximately 500 mm to 3000 mm. FIG. 2(c) illustrates a device for containing, reacting and measuring 23, and a method of containing, reacting and measuring according to the second embodiment.

[0060] The device for containing, reacting and measuring 23 has a pipette section 24 serving as the container section, a drawing and discharging section 25 for drawing and discharging into and from the pipette section 24, and a measuring devi...

third embodiment

[0075] Next, is a description of a device for containing, reacting and measuring 40 based on FIG. 3.

[0076]FIG. 3(a) schematically shows the device for containing, reacting and measuring 40 according to the third embodiment. This device for containing, reacting and measuring 40 uses another integrated carrier 42. The integrated carrier 42, as shown in FIG. 3(a) has the base member 15 rolled around a core 41 as the carrier.

[0077] As shown in FIG. 3(b), at opposite rims of the core 41 are respectively provided annular protruding portions 41a serving as the protective portions. By means of these protective portions, the base member 15 is bound so as not to come off from the core 41 being the carrier, and contact between an inner wall of a pipette section 44 serving as a later mentioned container section and the base member 15 is prevented, so that the liquid passing the surface of the base member 15 flows smoothly, and the integrated carrier 42 is positioned inside the container secti...

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Abstract

The invention relates to a device for containing, reacting and measuring, and a method of containing, reacting and measuring, and provides a device for containing, reacting and measuring, and a method of containing, reacting and measuring which is also able to effectively and quickly perform the reaction processing, measuring and identification. The invention comprises; a transparent container section having a liquid inlet / outlet and which is able to contain a base member with various substances for detection having predetermined chemical structures fixed at respective fixed positions which are arranged in a predetermined condition, and with each of the chemical structures associated with each of the fixed positions, a drawing and discharging section which is able to draw and discharge the liquid into and from the container section via the inlet / outlet, and a measuring device which is able to receive light from the contained base member, external to the container section and in a condition associated with the fixed position.

Description

[0001] The referenced application claims benefit to Japanese Patent Application No. 2001-034556, filed Feb. 9, 2001. TECHNICAL FIELD [0002] The present invention relates to a device for containing, reacting and measuring, and a method of containing, reacting and measuring. The invention relates to all manner of fields which require the handling of low molecular weight organisms and biopolymers such as genes, immune systems, amino acids, proteins and sugars, including for example the fields of engineering, agricultural science incorporating foodstuffs, agricultural production and seafood processing, pharmaceuticals, the medical field incorporating hygiene, health, immunity, disease and genetics, and scientific fields such as chemistry and biology. [0003] In particular, the present invention relates to a device for containing, reacting and measuring and a method of containing, reacting and measuring which is suitable for the analysis of genes including mutational analysis, polymorphic...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): B01L3/00B01L3/02C40B40/06C40B40/10C40B40/12C40B60/14G01N21/03G01N21/59G01N21/64G01N33/543G01N35/10
CPCB01J2219/00286Y10T436/2575B01J2219/00515B01J2219/00518B01J2219/0052B01J2219/00576B01J2219/00585B01J2219/00596B01J2219/00605B01J2219/00657B01J2219/00659B01J2219/00677B01J2219/00707B01J2219/00722B01J2219/00725B01J2219/00731B01J2219/00743B01L3/0275B01L3/502B01L2300/0636B01L2300/0812B01L2300/0832B01L2300/18B01L2400/0478C40B40/06C40B40/10C40B40/12C40B60/14G01N21/645G01N33/54366G01N2021/0325G01N2021/5973G01N2021/6484G01N2035/1055G01N2035/1062B01J2219/00353G01N33/53
Inventor TAJIMA, HIDEJI
Owner PSS BIO INSTR