Quantitative real-time assay for noroviruses and enteroviruses with built in quality control standard

US20060110724A1Inactive Publication Date: 2006-05-25HEALTH & HUMAN SERVICES GOVERNMENT OF THE US SEC DEPT OF
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Patent Information

Authority / Receiving Office
US · United States
Current Assignee / Owner
HEALTH & HUMAN SERVICES GOVERNMENT OF THE US SEC DEPT OF
Publication Date
2006-05-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

A method is provided for reverse transcription-polymerase chain reaction (RT-PCR) comprising a) amplifying a reverse transcribed cDNA in a mixture comprising Norovirus Genogroup I and Norovirus Genogroup II primers and probes, wherein said Norovirus primers and probes distinguish between Genogroup I and Genogroup II viruses; b) quantifying virus; and c) normalizing data based on a universal internal RNA control. Optionally, the method may also include primers and probes for Enteroviruses. A reaction mixture comprising Norovirus Genogroup I and Norovirus Genogroup II primers and probes, wherein said Norovirus primers and probes distinguish between Genogroup I and Genogroup II viruses and universal internal RNA control primers and probes are also included.
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Description

GOVERNMENTAL INTERESTS

[0001] This invention was developed employing support from the United States Food and Drug Administration. The Government of the United States of America may have certain rights in this invention.FIELD OF THE INVENTION

[0002] This invention relates to methods and reagents for detecting and quantifying viruses including Norovirus or Enterovirus by, for example, detecting or quantifying nucleic acids. BACKGROUND OF THE INVENTION

[0003] Noroviruses are estimated to be responsible for two-thirds of the non-bacterial food-borne illness and nearly all (96%) of the non-bacterial gastrointestinal illnesses each year in the United States. Norovirus infection occurs via the fecal-oral route from contaminated food such as oysters (Berg et al., 2000, J. Infect. Dis., 181:S381-S386; Shieh et al., 2000, J. Infect. Dis., 181: S360-S366), water (Yoder et al., 2004, Morb. Mortal. Wkly. Rep., 53(SS-8): 1-15; Blackburn et al., 2004, Morb Mortal Wkly Rep, 53(SS-8): 23-39) and eve...

Claims

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