Method for the detection of bacterial species of the genera anaplasma/ehrlichia and bartonella

Inactive Publication Date: 2009-01-01
INST DE SALUD CARLOS III
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Benefits of technology

[0010]The present invention relates to the use of genes 16S and msp2 and intergenic space 16S-23S for the detection and identification of the different bacterial species and groups of species that cau

Problems solved by technology

These circumstances, amongst others, entail the risk of introducing exotic diseases into the environment.
Said medical profiles, due to their high degree of similarity, do not allow a fast and reliable identification of the pathogenous agent, so that specific and fast treatment is not possible and is occasionally administered too late.
To date, the molecular diagnosis methods available are basically limited to the detection of pathogens based on antibody technology.
These types of techniques are scarcely applicable to certain species of genera, such as Bartonella and Anaplasma/Ehrlichia, due to the fact that said species do not normally grow in regular culture mediums and may even require cellular cultures.
Said technology,

Method used

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  • Method for the detection of bacterial species of the genera anaplasma/ehrlichia and bartonella
  • Method for the detection of bacterial species of the genera anaplasma/ehrlichia and bartonella
  • Method for the detection of bacterial species of the genera anaplasma/ehrlichia and bartonella

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[0015]According to a first aspect of the invention, said invention relates to a method (hereinafter, method of the invention) for the detection and identification, preferably simultaneous, of any of the bacterial species and genera, as indicated in Tables 2 and 3, comprising the following steps:[0016]a. Amplifying any of the sequences related to the group comprised of SEQ ID NO:1-17, 47 and / or their complementary sequences using specific triggers.[0017]b. Detecting the amplification of the sequences mentioned in step a), said amplification being indicative of the presence or absence of the bacterial genera or species that cause zoonosis, as indicated in Tables 2 and 3.

[0018]The triggers required to apply the method of the invention may be designed by means of multiple alignment with the sequences comprising SEQ ID NO:1-17 and 47 using computer programs such as CLUSTAL X, and allow the identification of highly preserved regions that will act as a mould for trigger design, which must ...

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Abstract

The present invention relates to a method for the detection and identification of bacterial species belonging to the genera Anaplasma/Ehrlichia and Bartonella, and also provides triggers and probes required for its application, as well as associated kits.

Description

[0001]This application claims the benefit of priority to Spanish Patent Application No. ES 200701830, filed Jun. 29, 2007, which is hereby incorporated by reference in its entirety.FIELD OF THE INVENTION[0002]The present invention relates to a method for the detection and identification of bacterial species belonging to the genera Anaplasma / Ehrlichia and Bartonella (ES 2264642), and also provides triggers and probes required for its application, as well as associated kits.BACKGROUND[0003]To date, about 200 zoonotic diseases (e.g., bartonelosis, leptospirosis, Lyme borreliosis, etc.), which affect humans and represent one of the main causes of death and entail substantial economic loss in third world countries, have been described. Coexistence with animals, lack of sanitary infrastructure and low cultural level continue to be the main allies of these diseases.[0004]In the same manner, certain types of zoonosis which are widespread in third world countries are now thriving in industri...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C07H21/04
CPCC12Q1/689C12Q2600/16C12R2001/01C12N1/205
Inventor ANDA FERNANDEZ, PEDROGIL GIL, HORACIOESCUDERO NIETO, RAQUELJADO GARCIA, ISABELRODRIGUEZ MORENO, ISABEL
Owner INST DE SALUD CARLOS III
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