HLA Class II Deficient Cells, HLA Class I Deficient Cells Capable of Expressing HLA Class II Proteins, and Uses Thereof

a technology deficient cells, applied in the field of hla class ii deficient cells and hla class i deficient cells capable of expressing hla class ii proteins, can solve the problems of time-consuming, expensive, and time-consuming process of human pluripotent stem cells and their derivatives
US20150056225A1Inactive Publication Date: 2015-02-26UNIV OF WASHINGTON +1
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Patent Information

Authority / Receiving Office
US · United States
Current Assignee / Owner
UNIV OF WASHINGTON
Publication Date
2015-02-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides isolated primate cells preferably human cells that comprise a genetically engineered disruption in a human leukocyte antigen (HLA) class II-related gene, which results in deficiency in MHC class II expression and function. This invention also provides isolated cells further comprising a genetically engineered disruption in a beta-2 microglobulin (B2M) gene, which results in HLA class I / class II deficiency. Also provided are the method of using the cells for transplantation and treating a disease condition.
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Description

CROSS REFERENCE

[0001] This application claims priority to U.S. provisional patent application Ser. No. 61 / 625,314 filed Apr. 17, 2012, incorporated by reference herein in its entirety.STATEMENT OF GOVERNMENT RIGHTS

[0002] This invention was made with government support under grant numbers R01GM086497 and R01DK55759 awarded by the National Institutes of Health. The government has certain rights in the invention.BACKGROUND OF THE INVENTION

[0003] Human pluripotent stem cells have the potential to treat diseases affecting almost every organ system. However, the clinical use of human pluripotent stem cells and their derivatives has a major limitation—rejection of transplanted cells by the recipient due to differences in the major histocompatibility complex.

[0004] The major histocompatibility complex (MHC) is a cell surface multi-component molecule found in all vertebrates that mediates interactions of leukocytes with other leukocytes or other cells. The MHC gene family is divided into three g...

Claims

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