Methods for detecting, diagnosing and treating endometrial cancer

a technology for endometrial cancer and metabolomics, applied in the field of biochemistry and medicine, can solve the problems that few studies in the literature have addressed the metabolomics of gynecologic cancer, and achieve the effects of improving prognosis, good diagnostic accuracy, and good diagnostic accuracy

Inactive Publication Date: 2017-01-05
WILLIAM BEAUMONT HOSPITAL
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  • Abstract
  • Description
  • Claims
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Benefits of technology

[0009]Using combined NMR and MS metabolomic analysis the inventors found statistically significant changes in the serum metabolome of patients diagnosed with EC compared with unaffected controls (normal). Of the total of 181 metabolites evaluated, four metabolites using NMR and fifty-three metabolites using Mass spectrometry showed significant changes in concentration in EC versus normals. Further, a combination of three metabolomic markers predicted the presence of EC with good diagnostic accuracy, AUC >0.80. Due to the significantly improved prognosis when EC is confined to the uterus, the inventors also investigated whether metabolomic markers could significantly distinguish early stage disease endometrial cancer, confined to the uterus (FIGO stages I and II) from unaffected patients. The metabolite algorithm achieved good diagnostic accuracy AUC >0.80.

Problems solved by technology

So far, few studies in the literature have addressed the metabolomics of gynecologic cancers.

Method used

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  • Methods for detecting, diagnosing and treating endometrial cancer
  • Methods for detecting, diagnosing and treating endometrial cancer
  • Methods for detecting, diagnosing and treating endometrial cancer

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example 1

Materials and Methods

[0084]Preoperative venous blood was collected from women diagnosed with EC at the Roswell Park Cancer Institute (Buffalo, N.Y.). The serum was stored at −80° C. and was not thawed until metabolomic analysis. All patients signed a written consent. The study protocol was approved by the IRB at RPCI. Specimens were collected as part of a biobanking project in which tissue and blood specimens of cancer patients are archived for future scientific study. Control specimens were obtained and archived from women without EC or other neoplastic disorders. Patient demographic and clinical information including age, race (Caucasian and African American), BMI, history of diabetes mellitus, use of hormonal replacement therapy and tamoxifen use were obtained. Disease staging based on the FIGO classification system was ascertained. For the sake of uniformity, only the endometriod histological type of EC was used. Further, only cases with no prior diagnosis or treatment of any ca...

example 2

Results

[0099]The inventors tested a total of 116 specimens, 56 with EC and 60 normal controls. A total of 181 metabolites, 149 based on DI-MS and 32 based on NMR were analyzed. This was after excluding metabolites with the percent of missing values in more than 20% of cases for both patient groups. Table 1 shows the demographic and clinical comparisons of EC and controls.

TABLE 1Endometrial Cancer versus Controls:Demographic and Clinical VariablesEndometrialNormalpParameterCancerControlsvalueNumber of cases5660Age, years, mean (SD)59.1(12.8)59.2(12.7)0.737#Racial origin, n (%)White52(92.9)56(93.3)1.000+Black4(7.1)4(6.7)Diabetes, n (%)13(23.2)2(3.3)0.002+BMI, mean (SD)36.9(17.3)28.8(6.8)0.002#HRT, n (%)13(23.2)20(33.3)0.227++Tamoxifen, n (%)1(1.8)3(5.0)0.619+Table 1:#t-test,+Fisher's Exact,++Pearson Chi-square

[0100]Unsurprisingly, there was a significantly higher frequency of diabetics and a higher mean body mass index (BMI) in EC compared to control cases. Spearman correlation analys...

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Abstract

The present invention relates to methods for detecting, diagnosing and/or treating endometrial cancer by detecting in a biological sample from a patient the levels of one or more of the metabolites: C14.2, PC ae C38:1, 3-Hydroxybutyric acid, C18:2, PC ae C40:1, and C6 (C4:1-DC). In some embodiments, the method also includes diagnosing the patient with endometrial cancer when the one or more metabolites in the biological sample is at a different level than a statistically validated threshold for the one or more metabolites, and ultrasound indicates endometrial cancer in the patient. In further embodiments, once endometrial cancer is diagnosed, the patient is treated for the endometrial cancer.

Description

CROSS REFERENCE TO RELATED APPLICATION[0001]This application claims priority to U.S. Ser. No. 62 / 185,602, filed Jun. 27, 2015. The entire contents of the aforementioned application are incorporated herein.FIELD OF THE INVENTION[0002]The present invention is in the field of biochemistry and medicine and relates to methods for detecting, diagnosing, and / or treating endometrial cancer.BACKGROUND OF THE INVENTION[0003]Metabolomics is the newest member of the “omics” systems biology discipline. In recent times there has been an explosion of publications related to the use of metabolomics for the analysis of complex disorders. Cancer analytics has been a principal focus. Applications and areas of promise for cancer metabolomics include early detection and disease staging. Further, metabolomics also has the potential to identify individuals who are likely to respond to particular cancer therapies (individualized medicine) and has proven of value in determining the effects of therapeutic ag...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/574A61B8/08A61B5/00
CPCG01N33/57442A61B5/055A61B5/4325A61B8/085A61B8/0858A61B8/12G01N2405/04G01N2570/00
Inventor BAHADO-SINGH, RAY
Owner WILLIAM BEAUMONT HOSPITAL
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