Efficient biochemical fulvic acid preparing process

Abstract

The efficient biochemical fulvic acid preparing process is to utilize, enrich and culture humic acid producing bacteria and auxiliary bacteria existing in nature to form specific fulvic acid producing bacteria. The fermented material includes crop stalks, saw dust and other agricultural and forest waste and some inorganic nutrient components as well as fulvic acid producing bacteria, and through controlling nutrients compounding ratio, water content, temperature, humidity and other technological parameters, fulvic acid is produced through directional fermentation. The present invention has the advantages of short fermentation period, low cost, high fulvic acid content, high product quality and easy control of the production process. The fermented product contains great amount of fulvic acid, amino acid, nucleic acid and other matters, and may be used widely in plantation, stockbreeding, aquiculture, industrial fermentation, medicine, pesticide and other industry.

Description

technical field

[0001] The invention relates to a high-efficiency preparation method of biochemical fulvic acid. Background technique

[0002] Fulvic acid is a kind of organic macromolecular compound with physiological activity, which has been widely used in industry, agriculture, forestry, animal husbandry, aquaculture, medical daily chemical and other industries. For a long time, people have relied on the extraction of humic acid substances from natural resources such as lignite and weathered coal, which not only has a complicated extraction process, but also consumes a large amount of coal resources. In recent years, people have successfully produced fulvic acid substances by imitating the formation conditions of humic acid substances in nature and using bio-fermentation technology, realizing the industrialization and large-scale production of fulvic acid substances. However, the current production process of biochemical fulvic acid is not standardized, and there are man...

Examples

Embodiment 1

[0036]Samples were collected from the above three different locations, and then several cultured strains were added. The active yeast was cultured in a wort shaker flask for 32-40 hours, and Trichoderma viride and white filamentous fungus were cultured in bran for 5 hours. ~7 days until the spores mature. The composition and weight ratio of the culture medium used for the expansion of the production strain are:

[0037] 15% corn flour, 12% soybean meal, 54% bran, 8% peat, 5% sawdust

[0038] Grease 2.2%, honey 3%, magnesium sulfate 0.3%, potassium dihydrogen phosphate 0.5%

[0039] The culture medium is sterilized by high temperature, and every 100 kilograms of medium is added with 1.5 kilograms of collected soil, 1 kilogram of fresh cow dung, 0.2 kilograms of cultured active yeast and 0.2 kilograms of bran spores, and ventilated culture for 48 hours can be used as biochemical yellow rot Acid bacteria use.

Embodiment 2

[0041] The organic nutritional components in the fermented material are prepared as follows: 40% sawdust, 22% stylosa powder, 10% brewer's grains, 10% bran, 8% corn flour, 5% oil, and 5% soybean meal.

[0042] Add 7 kg of inorganic nutritional components to every 100 kg of organic nutritional components, specifically: 4 kg of urea, 1.5 kg of potassium dihydrogen phosphate, 0.3 kg of magnesium sulfate, and 1.2 kg of diammonium phosphate.

[0043] After the fermentation materials are prepared, add 5 kg of cultivated biochemical fulvic acid strains, mix well, pack them into glass greenhouses for fermentation, and the specific fermentation process is as described above.

Embodiment 3

[0045] The organic nutritional components in the fermented material are formulated as follows: 32% sawdust, 40% peat, 12% bran, 8% corn flour, and 8% soybean.

[0046] Add 8.5 kg of inorganic nutritional components to every 100 kg of organic nutritional components, specifically: 1.5 kg of ammonium bicarbonate, 3.5 kg of urea, 2 kg of potassium dihydrogen phosphate, 0.5 kg of magnesium sulfate, and 1 kg of ammonium phosphate.

[0047] Fully dissolve the inorganic nutrient components in water and add them to the organic nutrient components, then add 3 kg of cultivated biochemical fulvic acid strains and 3 kg of fulvic acid mixture fermented last time, mix well and then ferment.

technical field

[0001] The invention relates to a high-efficiency preparation method of biochemical fulvic acid. Background technique

[0002] Fulvic acid is a kind of organic macromolecular compound with physiological activity, which has been widely used in industry, agriculture, forestry, animal husbandry, aquaculture, medical daily chemical and other industries. For a long time, people have relied on the extraction of humic acid substances from natural resources such as lignite and weathered coal, which not only has a complicated extraction process, but also consumes a large amount of coal resources. In recent years, people have successfully produced fulvic acid substances by imitating the formation conditions of humic acid substances in nature and using bio-fermentation technology, realizing the industrialization and large-scale production of fulvic acid substances. However, the current production process of biochemical fulvic acid is not standardized, and there are man...

Examples

Embodiment 1

[0036]Samples were collected from the above three different locations, and then several cultured strains were added. The active yeast was cultured in a wort shaker flask for 32-40 hours, and Trichoderma viride and white filamentous fungus were cultured in bran for 5 hours. ~7 days until the spores mature. The composition and weight ratio of the culture medium used for the expansion of the production strain are:

[0037] 15% corn flour, 12% soybean meal, 54% bran, 8% peat, 5% sawdust

[0038] Grease 2.2%, honey 3%, magnesium sulfate 0.3%, potassium dihydrogen phosphate 0.5%

[0039] The culture medium is sterilized by high temperature, and every 100 kilograms of medium is added with 1.5 kilograms of collected soil, 1 kilogram of fresh cow dung, 0.2 kilograms of cultured active yeast and 0.2 kilograms of bran spores, and ventilated culture for 48 hours can be used as biochemical yellow rot Acid bacteria use.

Embodiment 2

[0041] The organic nutritional components in the fermented material are prepared as follows: 40% sawdust, 22% stylosa powder, 10% brewer's grains, 10% bran, 8% corn flour, 5% oil, and 5% soybean meal.

[0042] Add 7 kg of inorganic nutritional components to every 100 kg of organic nutritional components, specifically: 4 kg of urea, 1.5 kg of potassium dihydrogen phosphate, 0.3 kg of magnesium sulfate, and 1.2 kg of diammonium phosphate.

[0043] After the fermentation materials are prepared, add 5 kg of cultivated biochemical fulvic acid strains, mix well, pack them into glass greenhouses for fermentation, and the specific fermentation process is as described above.

Embodiment 3

[0045] The organic nutritional components in the fermented material are formulated as follows: 32% sawdust, 40% peat, 12% bran, 8% corn flour, and 8% soybean.

[0046] Add 8.5 kg of inorganic nutritional components to every 100 kg of organic nutritional components, specifically: 1.5 kg of ammonium bicarbonate, 3.5 kg of urea, 2 kg of potassium dihydrogen phosphate, 0.5 kg of magnesium sulfate, and 1 kg of ammonium phosphate.

[0047] Fully dissolve the inorganic nutrient components in water and add them to the organic nutrient components, then add 3 kg of cultivated biochemical fulvic acid strains and 3 kg of fulvic acid mixture fermented last time, mix well and then ferment.