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36 results about "Cresol Red" patented technology
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Cresol red (full name: o-cresolsulfonephthalein) is a triarylmethane dye frequently used for monitoring the pH in aquaria.
Highly sensitive pH test paper and preparation method thereof
ActiveCN102519959AUniform and relatively firm adsorptionStrong adsorptionMaterial analysis by observing effect on chemical indicatorBromothymol bluePhenolphthalein
The invention relates to a piece of highly sensitive pH test paper and a preparation method thereof, wherein a developing strip is in the form of a piece of pH body paper, which is dipped in a reagent solution and then dried by hot air at constant temperature; the reagent solution contains an indicator; the formula of the indicator contains bromothymol blue, thymol blue, metanil yellow, m-methyl red, bromocresol green, nitrazine yellow, cresol red, phenolphthalein, para-nitrophenylazosalicylate sodium, titan yellow, fluorescent pigment orange red, 5% 4-nitrobenzenediazo-1-naphthol-3.6-sodium disulfonate solution and 30% dissolved ethanol aqueous solution; and the formula of the indicator further contains an aqueous colour fixing agent, a cotton wooden fibre mildew preventive, a special paper mildew preventive and a buffer neutralizer formed by modulating sodium hydroxide and phosphoric acid. The preparation method comprises the following steps of: adjusting the reagent solution till a pH value is up to 6-7.5, uniformly wetting the pH body paper, and drying through hot air at constant temperature. According to the invention, the reagent solution can be adjusted, so that the pH value is up to 6-7.5; steady and precise high sensitivity of the pH1-14 test paper is effectively increased; and the test paper is convenient and simple to use.
Owner:上海馨晟试化工科技有限公司
Reagent for detecting rice new-old degree and its detecting method
InactiveCN1641349ALow costSimple preparation processTesting starch susbtancesMaterial analysis by observing effect on chemical indicatorThymol blueMethyl palmoxirate
The invention relates to a rice metabolism detecting reagent and method, concretely used in the condition of detecting rice metabolism. It mainly takes and dissolves nitrazine yellow / chlorophenol red, p-nitrophenol, bromocresol green / bromothymol blue / resorcinol blue, bromocresol purple, and hematoxylin / cresol red / bromophenol red / methyl red into alcohol, and mixing them uniformly to obtain finished product of raw reagent liquid. As detecting, taking a raw reagent liquid and adding distilled water to dilute the raw reagent liquid so as to form a liquid reagent, adding rice to the liquid reagent to react, observing the colors of rice and solution, judging the metalolism degree of the rice detected, the metabolism situation and color of the rice corresponds to green and passes through yellow and salmon pink into red region, where the green expresses new rice, yellow expresses old rice and salmon pink expresses deeply aged rice. The reagent of the invention is lower-cost and has simple manufacturing process; the operation is quick, accurate and convenient; the detecting method is simple, able to be used in both qualitative detection and quantitative detection.
Owner:江苏省无锡江大大学科技园有限公司
Variable multicolor flour mud powder and method for producing the same
InactiveCN101195690APromotes fast digestionSatisfy the nature of playToysCITRATE ESTERBromothymol blue
The invention provides a children toy, in particular to a colorful-changeable flour-mud powder, according to mass percentages, which contains thymol phthalein of 0.05-0.06 accounts, fenolftaleina of 0.039-0.047 accounts, cresol red of 0.024-0.034 accounts, bromothymol blue of 0.015-0.025 accounts, anhydrous sodium carbonate of 2.95-3.35 accounts, citrate of 3.14-3.67 accounts and flour of 92.59-94.39 accounts. The preparation method comprises weighting and mixing indicator and anhydrous sodium carbonate uniformly, baking at 100DEG C for 20-30min, screening via 80-200 meshes, weighting and mixing citrate and flour uniformly, adding the screened power, packing, and obtaining the colorful-changeable flour-mud powder. The product can be added with water to be kneaded into mud form, with color change in kneading process. The invention has special formula, simple preparation and no hurt on human body, which color change function can develop children intelligence and develop practical ability.
Owner:SHANXI UNIV
One-stop PCR (Polymerase Chain Reaction) method
InactiveCN101921867AImprove efficiencyHigh sensitivityMicrobiological testing/measurementRibonucleosideEthanol precipitation
The invention relates to a novel PCR (Polymerase Chain Reaction) reaction method belonging to the field of biochemistry and molecular biology. The novel PCR reaction method sequentially comprises the following steps of: (1) preparing a compatible dye mixture, wherein the compatible dye mixture comprises ficoll 400, cresol red, lemon yellow and Gelred; (2) preparing a PCR principal mixture, wherein the PCR principal mixture comprises the compatible dye mixture, dNTP (Deoxy-ribonucleoside Triphosphate), a Taq DNA polymerase and a reaction buffer solution; (3) configuring a PCR reaction system; (4) setting and operating PCR reaction conditions; and (5) detecting PCR products. The novel PCR reaction method realizes the direct point sample electrophoresis and the immediate dyeing after PCR reaction; besides, the novel PCR reaction method uses a nontoxic nucleic acid dye and has low cost and high efficiency without toxicity; the obtained PCR products can be used for directly carrying out multiple downstream operations, such as ethanol precipitation, silica gel membrane product purification, and the like; and in addition, the novel PCR reaction method is suitable for the research of gene expression detection related to PCR, gene clone, and other aspects.
Owner:WUHAN UNIV
Preparation method of accurate pH test paper
ActiveCN103033511AHigh sensitivityGuaranteed accuracyMaterial analysis by observing effect on chemical indicatorMalachite greenBromocresol green
The invention relates to a preparation method of accurate pH test paper. An indicator is prepared by dissolving Metanil yellow, malachite green, acid turquoise blue, bromphenol blue, cresol red, m-cresol purple and bromocresol green in a 30% ethanol water solution. The preparation method comprises the following steps: previously impregnating base paper of pH test paper in the prepared indicator solution, and drying with heating steam of 95-105 DEG C at normal pressure. The preparation method of accurate pH test paper is simple in process, stable in product quality and higher in sensitivity and accuracy in comparison with the common accurate test paper, and the accuracy can be up to 0.2 level. Thus, the preparation method can be widely used in the fields of scientific research, industry, agriculture, environmental protection, medicine and food.
Owner:杭州特种纸业有限公司
Underlayer film-forming composition and pattern forming process
ActiveUS10228621B2Minimize reflectionSemiconductor/solid-state device manufacturingPhotosensitive material processingAlkaline waterLithographic artist
In lithography, a composition comprising a novolak resin comprising recurring units derived from a phenolphthalein, Phenol Red, Cresolphthalein, Cresol Red, or Thymolphthalein is used to form a photoresist underlayer film. The underlayer film is strippable in alkaline water, without causing damage to ion-implanted Si substrates or SiO2 substrates.
Owner:SHIN ETSU CHEM IND CO LTD
Method for determining sodium bicarbonate content in compound preparation
ActiveCN102890086AEasy to operateEliminate distractionsMaterial analysis by observing effect on chemical indicatorSodium bicarbonateBromocresol green
The invention provides a method for determining sodium bicarbonate content in compound preparation, which comprises steps of preparing sodium bicarbonate negative solution according to buffer base in a compound preparation prescription; determining sodium bicarbonate negative solution, determining a compound preparation sample, counting the sodium bicarbonate content and the like; according to the invention, the preparation of the sodium bicarbonate negative solution is increased; the sodium bicarbonate negative solution is corrected through a determined value; the interference of the buffer base such as sodium lactate and the like at an aspect of a content result is removed; in addition, novel color developing agent use technology is used; phenolphthalein-cresol red-thymol blue indicator is added based on original methyl red-bromocresol green indicator; the content of sodium carbonate and the sodum bicarbonate can be determined synchronously through the judgment of two ending points; simultaneously, color change is improved from the gradual change process of an original method into the sudden change process of a novel method. According to the invention, a content determining method is characterized by simple operation; and a determined result is precise, effective and stable.
Owner:HUAREN PHARMACEUTICAL CO LTD
Nucleic acid quantitative analysis method based on intelligent equipment assistance and application thereof
ActiveCN110265089AEasy to recordReduce analysis costsMicrobiological testing/measurementData visualisationColor changesIntelligent equipment
The invention provides a nucleic acid quantitative analysis method based on intelligent equipment assistance and application thereof. According to the method, through designing and constructing a nucleic acid amplification reaction system which is initiated by a nucleic acid object and is sensitive to pH, utilizing a pH indicator cresol red, and ensuring a color changing range between yellow 7.2-pink 8.8, visual quick analysis of the nucleic acid by naked eyes is realized. Based on the visual quick analysis, an intelligent device aided three-primary-color image scanning mode is utilized for realizing quick detection and quantitative analysis of the nucleic acid by intelligent device detecting terminal, wherein the used intelligent device has a photographing function, such as smart phone and panel computer.
Owner:UNIV OF SCI & TECH BEIJING
Early identifying method of pistachio male trees and pistachio female trees
InactiveCN102147400ASimple methodStart fastAnalysis using chemical indicatorsInsect diseaseAfter treatment
The invention relates to a chemical detecting method, in particular to an early identifying method of male pistachios and female pistachios. The early identifying method comprises the steps of: (1) collecting clean and non-insect-disease fresh leaves, respectively weighing 0.5 gram of clean male plant leaves without main veins and 0.5 gram of clean female plant leaves without main veins, shearing the leaves into the shape of pipe tobacco by adopting the leaf shearing method, putting the shorn leaves into a clean 50-milliliter triangular flask, adding a cresol red solution with the concentration of 0.1, 0.2 or 0.3 percent, putting the triangular flask into a constant-temperature box at the temperature of 28 DEG C, preserving the temperature for 12 to 72 hours, observing the change of color, and comparing with distilled water; and (2) judging the yellow red leaves after treatment as female plant leaves and the glaucous leaves as the male plant leaves so as to judge the female pistachio seeds and the male pistachio seeds. The early identifying method of male pistachios and female pistachios is rapid and effective, and is easy to operate and master.
Owner:李洁
Ureaplasma urealyticum/mycoplasma hominis combined rapid culture and drug sensitivity detection kit
InactiveCN104988206AResolve detectionResolve accuracyMicrobiological testing/measurementMicroorganism based processesPenicillinArginine
Owner:姜洪波 +1
Special ammonia indicator for analyzing ammonia content by flow injection instruments and preparation thereof
InactiveCN101587128AConsistent stabilityMeet the measurement requirementsColor/spectral properties measurementsMass ratioInstrumentation
The invention belongs to the technical field of agricultural analysis, specifically relates to a special ammonia indicator for analyzing ammonia content by flow injection instruments. An import flow injection instrument is used for analyzing the ammonia contents of samples such as animal and plant life, food, water and soil, which has been normally-used instrument device and technology in domestic universities and colleges as well as scientific research, animal and plant life and food detection and check units. However, the most important reagent that is ammonia indicator for analyzing ammonia content was monopolized by foreign companies all the time, and the cost is expensive. The invention provides the proper mass ratio of prepared special ammonia indicators of flow injection instruments that are cresol red, bromcresol purple and bromothymol blue. The main technical targets of the special ammonia indicator in the invention all meet analysis requirements, and the cost of the reagent is 3.3% of that of contrast reagent.
Owner:HUAZHONG AGRI UNIV
Biological sample nucleic acid release preservative
The invention discloses a biological sample nucleic acid release preservative, which belongs to the technical field of molecular biology, and discloses a method which can split common detection samples, can be directly used for downstream PCR detection or high-throughput sequencing and can stably preserve sample nucleic acid released after splitting for a long time. The preservation agent comprises Tween20 (0.01%-10%, V / V), glycogen (2-500 ng / mu l), formamide (2-500 ng / mu l), artificially synthesized 25 nt poly A (2-500 ng / mu l), potassium hydroxide (0.01 mM-50 mM) and cresol red (0.01%-10%, mass / volume), a solvent is nuclease-free water. By means of the method, quality control can be conducted on a PCR system through naked eyes, consumed time is short, efficiency is high, operation is easy and convenient, production cost is low, preservation time is long, and the preservation agent has great market popularization value.
Owner:JIANGSU GENESTONE BIO-TECH CO LTD
Thermosensitive time indicating device
The invention relates to a thermosensitive time indicating device. The thermosensitive time indicating device comprises a base layer, a first substrate layer which forms an accommodation body with the base layer, a volatile functional layer which is accommodated in the accommodation body and comprises a phase change material selected from at least one of oleic acid, elaidic acid, n-nonanoic acid, octanoic acid, glyceryl triacetate and lauric acid, an adsorption indicating layer which is formed on the surface of the first substrate layer and contains an indicator composition, and a second substrate layer which is formed on the surface of the adsorption indicating layer and has the advantages of transparency and airtightness. The indicator composition comprises at least one of a pH sensitive dye. The pH sensitive dye is selected from at least one of m-cresol violet, anthocyanins, thymolphidin, o-cresolphthalein, bromothymol blue, cresol red, cetyltrimethylammonium cation, neutral red, phenol red, rhodamine, sulfo rhodamine 101 and thymol blue. The thermosensitive time indicating device is simple in structure, and is advantageous for miniaturization.
Owner:SHENZHEN NINE STARS PRINTING & PACKAGING GRP
Method for measuring lanthanum content in lanthanum-iron alloy
The invention discloses a method for measuring the lanthanum content in a lanthanum-iron alloy. The method comprises the following steps: dissolving a lanthanum-iron alloy by nitric acid; diluting the solution by water, boiling the diluted solution; then adding hot oxalic acid, dropwise adding 5 drops of cresol red indicator at the same time, adjusting the pH value by ammonia liquor until the color turns from red to yellow, heating the solution to a temperature of 100 DEG C, maintaining the temperature for 10 minutes; allowing the solution to stand still for two hours, filtering the solution by a quantitative filter paper, fully washing the precipitate by a mixed water solution of oxalic acid and ammonium oxalate, transferring the precipitate to a filter paper, washing the precipitate by a mixed solution of oxalic acid and ammonium oxalate, placing the precipitate into a ceramic crucible, burning the precipitate into ashes; finally burning the ashes in a muffle furnace for 30 to 40 minutes, cooling, and weighing. The lanthanum content can be calculated according to a formula: WLa2O3=m1*100% / m, wherein the m1 represents the mass of rare earth oxides, the measurement unit of m1 is g, m represents the mass of a sample, and the measurement unit is g. The cresol red indicator is a ethanol solution of cresol red. The provided method has the advantages of short course, low cost, rapidness, efficiency, accuracy, and good stability, and can be easily mastered.
Owner:INNER MONGOLIA BAOTOU STEEL UNION
Cresol red colorimetric method for soil respiration measurement and measurement device
InactiveCN105466870AHigh sensitivityImprove securityColor/spectral properties measurementsMeasurement deviceLength wave
The invention discloses a cresol red colorimetric method for soil respiration measurement and a measurement device. The measurement device comprises a plastic test tube containing a cresol red reagent and a sealing cover. The plastic test tube and soil to be measured are sealed in the sealing cover, the absorbancy of the cresol red reagent is measured with the colorimetric method under the wavelength of 560 nm after respiration, and soil respiration intensity is measured through a standard curve. By preparing the cresol red reagent from the acid-base indicator cresol red, neutral salt and bicarbonate to absorb CO2 in soil, cultured soil respiration intensity is measured, and field soil respiration intensity can also be measured; strong base such as NaOH with high corrosivity is not needed, the reagent can be used repeatedly, sensitivity and safety performance are high, special or expensive instruments are not needed, the operation method is convenient and quick, and test data are reliable and accurate. The measurement device also has the advantages of being simple in structure, low in price, and convenient and rapid to use and operate.
Owner:GUIZHOU UNIV
Thermochromic material, preparation method thereof and application of thermochromic material in cigarette paper
The invention discloses a thermochromic material, which is a system of hollow mesoporous silica loaded cresol red-boric acid, or a system of amino-modified hollow mesoporous silica loaded cresol red-boric acid. The invention further discloses a preparation method of the thermochromic material and application of the thermochromic coating material in cigarette paper. The thermochromic material disclosed by the invention has a prompting effect on temperature.
Owner:CHINA TOBACCO YUNNAN IND
Highly sensitive pH test paper and preparation method thereof
ActiveCN102519959BUniform and relatively firm adsorptionImprove adsorption capacityMaterial analysis by observing effect on chemical indicatorPhenolphthaleinBromothymol blue
The invention relates to a piece of highly sensitive pH test paper and a preparation method thereof, wherein a developing strip is in the form of a piece of pH body paper, which is dipped in a reagent solution and then dried by hot air at constant temperature; the reagent solution contains an indicator; the formula of the indicator contains bromothymol blue, thymol blue, metanil yellow, m-methyl red, bromocresol green, nitrazine yellow, cresol red, phenolphthalein, para-nitrophenylazosalicylate sodium, titan yellow, fluorescent pigment orange red, 5% 4-nitrobenzenediazo-1-naphthol-3.6-sodium disulfonate solution and 30% dissolved ethanol aqueous solution; and the formula of the indicator further contains an aqueous colour fixing agent, a cotton wooden fibre mildew preventive, a special paper mildew preventive and a buffer neutralizer formed by modulating sodium hydroxide and phosphoric acid. The preparation method comprises the following steps of: adjusting the reagent solution till a pH value is up to 6-7.5, uniformly wetting the pH body paper, and drying through hot air at constant temperature. According to the invention, the reagent solution can be adjusted, so that the pH value is up to 6-7.5; steady and precise high sensitivity of the pH1-14 test paper is effectively increased; and the test paper is convenient and simple to use.
Owner:上海馨晟试化工科技有限公司
One-stop PCR (Polymerase Chain Reaction) method
InactiveCN101921867BIncrease success rateEasy to prepareMicrobiological testing/measurementRibonucleosideEthanol precipitation
The invention relates to a novel PCR (Polymerase Chain Reaction) reaction method belonging to the field of biochemistry and molecular biology. The novel PCR reaction method sequentially comprises the following steps of: (1) preparing a compatible dye mixture, wherein the compatible dye mixture comprises ficoll 400, cresol red, lemon yellow and Gelred; (2) preparing a PCR principal mixture, wherein the PCR principal mixture comprises the compatible dye mixture, dNTP (Deoxy-ribonucleoside Triphosphate), a Taq DNA polymerase and a reaction buffer solution; (3) configuring a PCR reaction system; (4) setting and operating PCR reaction conditions; and (5) detecting PCR products. The novel PCR reaction method realizes the direct point sample electrophoresis and the immediate dyeing after PCR reaction; besides, the novel PCR reaction method uses a nontoxic nucleic acid dye and has low cost and high efficiency without toxicity; the obtained PCR products can be used for directly carrying out multiple downstream operations, such as ethanol precipitation, silica gel membrane product purification, and the like; and in addition, the novel PCR reaction method is suitable for the research of geneexpression detection related to PCR, gene clone, and other aspects.
Owner:WUHAN UNIV
A kind of spill-proof test paper box for rapid detection of various heavy metal ions
ActiveCN104713877BAvoid bendingAvoid damageMaterial analysis by observing effect on chemical indicatorSolid structureBiochemical engineering
The invention discloses an anti-overflow type multi-heavy metal ion rapid detection test paper box. The anti-overflow type multi-heavy metal ion rapid detection test paper box comprises a shell and a press cover matched with the shell, wherein the shell is of a rectangular solid structure with open top, a buffer air cushion is arranged in the shell, an adhesive sticker layer is arranged on the buffer air cushion, and is sequentially provided with limiting strips against each other in an end-to-end way, a urease immobilized cellulose acetate membrane, water soluble release paper, urea and cresol red immobilized developing and water-absorbing filter paper and water-absorbing paper from left to right, a sample solution dropping-in hole is formed in the position at which the press cover corresponds to the cellulose acetate membrane, a liquid storage cavity is formed in the press cover, and is communicated with the sample solution dropping-in hole by virtue of an overflow channel, and an observation window is formed at the position at which the press cover corresponds to the developing and water-absorbing filter paper. The anti-overflow type multi-heavy metal ion rapid detection test paper box is simple in structure, does not overflow and is easy to use and carry, developing is rapid and uniform, the detection efficiency is high, and the detection results are accurate.
Owner:上海谱诺检测技术有限公司
Preparation method of accurate pH test paper
ActiveCN103033511BHigh sensitivityGuaranteed accuracyMaterial analysis by observing effect on chemical indicatorMalachite greenBromocresol green
The invention relates to a preparation method of accurate pH test paper. An indicator is prepared by dissolving Metanil yellow, malachite green, acid turquoise blue, bromphenol blue, cresol red, m-cresol purple and bromocresol green in a 30% ethanol water solution. The preparation method comprises the following steps: previously impregnating base paper of pH test paper in the prepared indicator solution, and drying with heating steam of 95-105 DEG C at normal pressure. The preparation method of accurate pH test paper is simple in process, stable in product quality and higher in sensitivity and accuracy in comparison with the common accurate test paper, and the accuracy can be up to 0.2 level. Thus, the preparation method can be widely used in the fields of scientific research, industry, agriculture, environmental protection, medicine and food.
Owner:杭州特种纸业有限公司
Method for detecting disinfection liquid residue on explant surface during tissue culture
InactiveCN106568766AGuaranteed survival rateDetermining the extent of residue removalMaterial analysis by observing effect on chemical indicatorHorticulture methodsCresolAlcohol
The embodiments of the present invention disclose a method for detecting the disinfection liquid residue on the explant surface during tissue culture. The method comprises: washing: washing explant surface to obtain a washed explant; disinfecting: soaking the washed explant in 70% alcohol and a 5-10% NaClO solution; disinfecting liquid residue removing: rinsing the disinfected explant by using sterile water; and detecting: adding a cresol red indicator to the last sterile water washing waste liquid in a dropwise manner, and observing the color of the waste liquid to determine the residue. According to the present invention, the washing agent is rationally used in the explant disinfecting method , and the explant is subjected to a series of the disinfecting operations and the detection step by matching the cleaning, detecting and observing technology; and the detection method is simple and easy to perform, does not cause any added damage to the explant, can accurately determine the disinfecting liquid residue removing degree, and improves the production efficiency under the premise of the ensuring of the explant survival rate.
Owner:DINGZHOU LYUGU AGRI TECH DEV CO LTD
Method and detecting tube for detecting soybean urease activity
ActiveCN107177664ASimple structureSampling quality is stableMicrobiological testing/measurementNational standardColor changes
The invention provides a soybean urease activity detecting tube which comprises a urea cresol red sodium salt extracting fluid tube, a sample quantifying tube and a bottle cap which are sequentially connected. The detecting tube has simple structure and stable sampling quality and avoids large fluctuation. The invention further provides a method for detecting soybean urease activity. The method comprises the steps of adding a soybean meal sample into the sample quantifying tube, connecting the sample quantifying tube with the top of the urea cresol red sodium salt extracting fluid tube, shocking, recording color change time and calculating a soybean urease activity value of the soybean meal sample according to a corresponding fitted equation. The detecting method has simple steps, short consumed time in a detecting process and not high requirements for skilled degree of operators and equipment; furthermore, detecting results are identical with detection results of a national-standard method.
Owner:HENAN UNIVERSITY OF TECHNOLOGY
A nucleic acid quantitative analysis method based on intelligent equipment and its application
ActiveCN110265089BReduce analysis costsRealize quantitative analysisMicrobiological testing/measurementData visualisationEngineeringOrganic chemistry
The invention provides a nucleic acid quantitative analysis method based on intelligent equipment and its application. The method is designed to construct a pH-sensitive nucleic acid amplification reaction system triggered by a nucleic acid target, using a pH indicator cresol red, and the color change range is Yellow 7.2-pink 8.8 realizes the visual and rapid analysis of nucleic acids with the naked eye; on this basis, using the three-primary-color image scanning mode assisted by smart devices, the rapid detection and quantitative analysis of nucleic acids are realized with smart device detection terminals. Devices include smartphones, tablets and other smart devices with camera functions.
Owner:UNIV OF SCI & TECH BEIJING
Soybean milk ripeness degree rapid detection card and detection method thereof
PendingCN112858276AReduced hiding effectEasy to operateMaterial analysis by observing effect on chemical indicatorBiotechnologyPolyester
The invention discloses a soybean milk ripeness degree rapid detection card and a detection method thereof, and relates to the field of soybean milk ripeness degree detection. The soybean milk ripeness degree rapid detection card comprises a bottom plate, wherein a sample pad, a reagent pad, a color development pad and a water absorption pad are sequentially pasted on the bottom plate from left to right, the sample pad is made of polyester fibers, the sample pad is soaked in a phosphate buffer solution and dried for later use, the reagent pad is impregnated with a urea solution and dried for later use, and cresol red with a mass percent of 0.5% is dipped on the color development pad. According to the invention, the soybean milk ripeness degree rapid detection card has filtering and impurity removing functions, wherein pigments and small particles in soybean milk liquid to be detected can be filtered and adsorbed, so that the covering effect of the background color of the soybean milk on the water absorbing paper is reduced, and the soybean milk ripeness degree rapid detection card is suitable for pure soybean milk, five-cereal soybean milk, black soybean milk and other soybean milk with various colors in the market added with red dates, Chinese wolfberry fruits and the like; and the method is easy to operate, high in sensitivity, applicable in technology, low in cost, safe to operate, free of flammable and explosive solutions, non-toxic, harmless and free of organic reagents.
Owner:郑州欧柯奇仪器制造有限公司
Preparation process for cresol red
InactiveCN103351374AHigh purityMeet the requirements of reducing production costsOrganic chemistryParticulatesFiltration
The invention specifically discloses a preparation process for cresol red, which belongs to the field of synthetic technology of indicators. The preparation process comprises the following steps: 1, with o-benzoyl sulfonic anhydride, o-benzoyl and zinc chloride anhydrous in a mass ratio of 1: 1-2: 1-2.5 as raw materials, controlling reaction temperature to be 100 to 110 DEG C and reaction time to be 3 to 4 h and adding hydrochloric acid and water with slow stirring so as to prepare a tiny particle; 2, subjecting the tiny particle prepared in the step 1 to filtration and deposition in warm water with a temperature of 45 to 60 DEG C; and 3, successively subjecting a sediment of filtration obtained in step 2 to heating, drying and cooling, adding diethyl ether and benzene, carrying out slow stirring for 15 to 30 m and carrying out filtration and drying so as to finally prepare cresol red. The preparation process for cresol red provided by the invention overcomes the problems of low efficiency and low purity of conventional processing and preparation technology, meets demands of middle and small-sized enterprises for synthesis technology and can rapidly and easily process and prepare cresol red with high purity.
Owner:TAICANG HENGYI MEDICINE CHEM MATERIAL FACTORY
Determination method of total selenium, inorganic selenium and organic selenium content of yeast selenium
ActiveCN104931468BAvoid volatile lossReduce dosageFluorescence/phosphorescenceDigestionInorganic selenium
Owner:ZHEJIANG UNIV
Technology for preparing semi-xylenol orange by one-step method
InactiveCN101397290ASimple processEliminate secondary purificationOrganic chemistryAcetic acidIminodiacetic acid
The invention relates to a method for preparing semixylenol orange by one-step operation, including the following steps: (1) cresol red, iminodiacetic acid disodium salt and glacial acetic acid are mixed and stirred evenly, the mixture is heated till full dissolving at the temperature of 60 to 65 DEG C and then is cooled to 25 to 30 DEG C to obtain a cooled mixed liquid; (2) 37 percent of formaldehyde solution is added into the cooled mixture which is rested for 7 to 8 hours and then is constantly stirred for 7 to 8 hours at the temperature of 60 to 65 DEG C; (3) the unwanted solution is removed by decompression and the remaining black thick matter is poured into an evaporation tank to be steamed to be dry at the steaming temperature of 70 to 80 DEG C to form crystals; and (4) the crystals are crushed to obtain the orange-red solid-semixylenol orange finished products. The one-step synthesizing method adopted by the invention greatly enhances the conversion rate; the yield is higher; the sensitivity reaches the standard of similar products; the method is suitable for the large-scale industrial production.
Owner:TIANJIN CHEM REAGENT RES INST
Chloroethylene detection reagent and preparation method thereof
InactiveCN104949975AReduce distractionsAccurate measurementMaterial analysis by observing effect on chemical indicatorPhosphomolybdic acidSodium sulfate
The invention discloses a chloroethylene detection reagent and a preparation method thereof, and belongs to the technical field of gas detection. The detection reagent comprises the following components in parts by weight: 15-25 parts of phosphomolybdic acid, 5-10 parts of dimethyl yellow, 2-9 parts of sodium sulfate, 3-7 parts of cresol red, 2-5 parts of tricresyl phosphate, 2-6 parts of vulcanized zinc dimethyl dithiocarbamate, 10-25 parts of dimethylacetamide and 15-25 parts of deionized water. The preparation method comprises the steps: (1) preparing a pre-mixed liquid; (2) obtaining a clear mixed liquid; and (3) standing under a vacuum condition for 12-48h, and sealing so as to obtain the chloroethylene detection reagent. The chloroethylene detection reagent provided by the invention is easy to operate, high in sensitivity and high in measurement accuracy and can be used for effectively detecting the content of chloroethylene in air.
Owner:SUZHOU YOUJUN ENVIRONMENTAL SCI & TECH
Fluorescent dye and electrophoresis PCR (Polymerase Chain Reaction) dual-purpose buffer solution
The invention discloses a fluorescent dye and electrophoresis PCR (Polymerase Chain Reaction) dual-purpose buffer solution which comprises the following components: (1.5-2.5)*10<-5>g / L of sodium cresol red, 71-75% of PCR Buffer, 5.5-6.5% of dNTP, 4-5% of glycerin, 0.008-0.015% of SYBR Green I and 15-20% of sterilized deionized water. The dual-purpose dye has universality to different detection platforms, can be applicable to Taq polymerase of different brands, is at least confirmed to be applied to Invitrogen, Promega, Bioer Taq and pfuTaq, and can be simultaneously applied to Syber green I real-time PCR amplification or general PCR amplification. Moreover, agarose electrophoresis can be directly performed to execute product recovery or interpretation after the fluorescent PCR amplification, electrophoresis can be performed without adding an extra dye, electrophoretic determination can also be performed after fluorescent PCR detection, and no extra dyeing agent is need to be added. According to a formula of the buffer solution, two processes of diluting the dye and adding nucleic acid dyes during electrophoretic procedures can be simplified, particularly the traditional nucleic acid dye EtBr and other cancerogens are omitted, and the test safety is greatly improved.
Owner:江苏中济万泰生物医药有限公司
Infiltration water and inorganic solute global distribution test method under micro-spraying irrigation condition
ActiveCN112345413AAccurate measurementEvaluate global infiltration redistributionMachine part testingSurface/boundary effectWater useMethyl blue
The invention discloses an infiltration water and inorganic solute global distribution test method under a micro-spraying irrigation condition. The method comprises the following steps: arranging a test area, and measuring physical and hydrodynamic parameter properties of soil; dissolving an inorganic solute solution in water to serve as a tracer agent, and carrying out a micro-spraying experimentin a test area; preparing a cresol red-methylene blue mixed color developing solution; after 24-48 hours, digging out an area which is not sprayed, forming a section layer by layer along the axis direction, and measuring the soil moisture content at different positions and different depths and the concentration distribution of a color developing area; determining the soil moisture content variation of the color developing area according to the measured soil moisture content, and determining the satisfaction degree of spray irrigation water and inorganic solute redistribution on irrigation requirements according to the variation of the moisture content and the concentration distribution of the color developing area at different depth positions. According to the method, the micro-spraying irrigation efficiency can be systematically evaluated by measuring the redistribution of water and inorganic solutes under the condition of micro-spraying irrigation; and a basis is provided for improving the micro-spraying irrigation level and realizing precise agricultural water.
Owner:WUHAN UNIV
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