35 results about "Cresol Red" patented technology

The invention relates to a piece of highly sensitive pH test paper and a preparation method thereof, wherein a developing strip is in the form of a piece of pH body paper, which is dipped in a reagent solution and then dried by hot air at constant temperature; the reagent solution contains an indicator; the formula of the indicator contains bromothymol blue, thymol blue, metanil yellow, m-methyl red, bromocresol green, nitrazine yellow, cresol red, phenolphthalein, para-nitrophenylazosalicylate sodium, titan yellow, fluorescent pigment orange red, 5% 4-nitrobenzenediazo-1-naphthol-3.6-sodium disulfonate solution and 30% dissolved ethanol aqueous solution; and the formula of the indicator further contains an aqueous colour fixing agent, a cotton wooden fibre mildew preventive, a special paper mildew preventive and a buffer neutralizer formed by modulating sodium hydroxide and phosphoric acid. The preparation method comprises the following steps of: adjusting the reagent solution till a pH value is up to 6-7.5, uniformly wetting the pH body paper, and drying through hot air at constant temperature. According to the invention, the reagent solution can be adjusted, so that the pH value is up to 6-7.5; steady and precise high sensitivity of the pH1-14 test paper is effectively increased; and the test paper is convenient and simple to use.

The invention relates to a rice metabolism detecting reagent and method, concretely used in the condition of detecting rice metabolism. It mainly takes and dissolves nitrazine yellow/chlorophenol red, p-nitrophenol, bromocresol green/bromothymol blue/resorcinol blue, bromocresol purple, and hematoxylin/cresol red/bromophenol red/methyl red into alcohol, and mixing them uniformly to obtain finished product of raw reagent liquid. As detecting, taking a raw reagent liquid and adding distilled water to dilute the raw reagent liquid so as to form a liquid reagent, adding rice to the liquid reagent to react, observing the colors of rice and solution, judging the metalolism degree of the rice detected, the metabolism situation and color of the rice corresponds to green and passes through yellow and salmon pink into red region, where the green expresses new rice, yellow expresses old rice and salmon pink expresses deeply aged rice. The reagent of the invention is lower-cost and has simple manufacturing process; the operation is quick, accurate and convenient; the detecting method is simple, able to be used in both qualitative detection and quantitative detection.

The invention provides a children toy, in particular to a colorful-changeable flour-mud powder, according to mass percentages, which contains thymol phthalein of 0.05-0.06 accounts, fenolftaleina of 0.039-0.047 accounts, cresol red of 0.024-0.034 accounts, bromothymol blue of 0.015-0.025 accounts, anhydrous sodium carbonate of 2.95-3.35 accounts, citrate of 3.14-3.67 accounts and flour of 92.59-94.39 accounts. The preparation method comprises weighting and mixing indicator and anhydrous sodium carbonate uniformly, baking at 100DEG C for 20-30min, screening via 80-200 meshes, weighting and mixing citrate and flour uniformly, adding the screened power, packing, and obtaining the colorful-changeable flour-mud powder. The product can be added with water to be kneaded into mud form, with color change in kneading process. The invention has special formula, simple preparation and no hurt on human body, which color change function can develop children intelligence and develop practical ability.

The invention relates to a novel PCR (Polymerase Chain Reaction) reaction method belonging to the field of biochemistry and molecular biology. The novel PCR reaction method sequentially comprises the following steps of: (1) preparing a compatible dye mixture, wherein the compatible dye mixture comprises ficoll 400, cresol red, lemon yellow and Gelred; (2) preparing a PCR principal mixture, wherein the PCR principal mixture comprises the compatible dye mixture, dNTP (Deoxy-ribonucleoside Triphosphate), a Taq DNA polymerase and a reaction buffer solution; (3) configuring a PCR reaction system; (4) setting and operating PCR reaction conditions; and (5) detecting PCR products. The novel PCR reaction method realizes the direct point sample electrophoresis and the immediate dyeing after PCR reaction; besides, the novel PCR reaction method uses a nontoxic nucleic acid dye and has low cost and high efficiency without toxicity; the obtained PCR products can be used for directly carrying out multiple downstream operations, such as ethanol precipitation, silica gel membrane product purification, and the like; and in addition, the novel PCR reaction method is suitable for the research of gene expression detection related to PCR, gene clone, and other aspects.

The invention relates to a chemical detecting method, in particular to an early identifying method of male pistachios and female pistachios. The early identifying method comprises the steps of: (1) collecting clean and non-insect-disease fresh leaves, respectively weighing 0.5 gram of clean male plant leaves without main veins and 0.5 gram of clean female plant leaves without main veins, shearing the leaves into the shape of pipe tobacco by adopting the leaf shearing method, putting the shorn leaves into a clean 50-milliliter triangular flask, adding a cresol red solution with the concentration of 0.1, 0.2 or 0.3 percent, putting the triangular flask into a constant-temperature box at the temperature of 28 DEG C, preserving the temperature for 12 to 72 hours, observing the change of color, and comparing with distilled water; and (2) judging the yellow red leaves after treatment as female plant leaves and the glaucous leaves as the male plant leaves so as to judge the female pistachio seeds and the male pistachio seeds. The early identifying method of male pistachios and female pistachios is rapid and effective, and is easy to operate and master.

Provided is a ureaplasma urealyticum/mycoplasma hominis combined rapid culture and drug sensitivity detection kit. The kit can complete detection in 8-24 h, and meanwhile, drug-resistant detection of various antibiotics can be performed. The combined rapid culture kit is mainly composed of pork stomach digestive juice or lung digestive juice, a beef extracting solution or a beef heart extracting solution, cattle serum or horse serum or other animal serum, sodium chloride, peptone, yeast powder, urea, L-arginine, phenol red indicator or cresol red indicator, and penicillin or other antibiotics. The drug detection kit can detect doxycycline hyclate, roxithromycin, acetylspiramycin, gatifloxacin, clarithromycin, azithromycin, clindamycin, erythromycin, kitasamycin, ofloxacin, levofloxacin hydrochloride, sparfloxacin, ciprofloxacin, doxycycline, erythromycin cydocarbonate and various other antibiotics. By means of the detection kit, not only is the mycoplasma detection time shortened, but also drug-resistant detection can be performed at the same time so that clinical medication can be guided. The detection kit has the beneficial effects of being high in sensitivity, high in specificity, high in detection rate, easy and convenient to operate and the like.

The invention belongs to the technical field of agricultural analysis, specifically relates to a special ammonia indicator for analyzing ammonia content by flow injection instruments. An import flow injection instrument is used for analyzing the ammonia contents of samples such as animal and plant life, food, water and soil, which has been normally-used instrument device and technology in domestic universities and colleges as well as scientific research, animal and plant life and food detection and check units. However, the most important reagent that is ammonia indicator for analyzing ammonia content was monopolized by foreign companies all the time, and the cost is expensive. The invention provides the proper mass ratio of prepared special ammonia indicators of flow injection instruments that are cresol red, bromcresol purple and bromothymol blue. The main technical targets of the special ammonia indicator in the invention all meet analysis requirements, and the cost of the reagent is 3.3% of that of contrast reagent.

The invention discloses a biological sample nucleic acid release preservative, which belongs to the technical field of molecular biology, and discloses a method which can split common detection samples, can be directly used for downstream PCR detection or high-throughput sequencing and can stably preserve sample nucleic acid released after splitting for a long time. The preservation agent comprises Tween20 (0.01%-10%, V/V), glycogen (2-500 ng/mu l), formamide (2-500 ng/mu l), artificially synthesized 25 nt poly A (2-500 ng/mu l), potassium hydroxide (0.01 mM-50 mM) and cresol red (0.01%-10%, mass/volume), a solvent is nuclease-free water. By means of the method, quality control can be conducted on a PCR system through naked eyes, consumed time is short, efficiency is high, operation is easy and convenient, production cost is low, preservation time is long, and the preservation agent has great market popularization value.

The invention relates to a thermosensitive time indicating device. The thermosensitive time indicating device comprises a base layer, a first substrate layer which forms an accommodation body with the base layer, a volatile functional layer which is accommodated in the accommodation body and comprises a phase change material selected from at least one of oleic acid, elaidic acid, n-nonanoic acid, octanoic acid, glyceryl triacetate and lauric acid, an adsorption indicating layer which is formed on the surface of the first substrate layer and contains an indicator composition, and a second substrate layer which is formed on the surface of the adsorption indicating layer and has the advantages of transparency and airtightness. The indicator composition comprises at least one of a pH sensitive dye. The pH sensitive dye is selected from at least one of m-cresol violet, anthocyanins, thymolphidin, o-cresolphthalein, bromothymol blue, cresol red, cetyltrimethylammonium cation, neutral red, phenol red, rhodamine, sulfo rhodamine 101 and thymol blue. The thermosensitive time indicating device is simple in structure, and is advantageous for miniaturization.

The invention discloses a method for measuring the lanthanum content in a lanthanum-iron alloy. The method comprises the following steps: dissolving a lanthanum-iron alloy by nitric acid; diluting the solution by water, boiling the diluted solution; then adding hot oxalic acid, dropwise adding 5 drops of cresol red indicator at the same time, adjusting the pH value by ammonia liquor until the color turns from red to yellow, heating the solution to a temperature of 100 DEG C, maintaining the temperature for 10 minutes; allowing the solution to stand still for two hours, filtering the solution by a quantitative filter paper, fully washing the precipitate by a mixed water solution of oxalic acid and ammonium oxalate, transferring the precipitate to a filter paper, washing the precipitate by a mixed solution of oxalic acid and ammonium oxalate, placing the precipitate into a ceramic crucible, burning the precipitate into ashes; finally burning the ashes in a muffle furnace for 30 to 40 minutes, cooling, and weighing. The lanthanum content can be calculated according to a formula: WLa2O3=m1*100%/m, wherein the m1 represents the mass of rare earth oxides, the measurement unit of m1 is g, m represents the mass of a sample, and the measurement unit is g. The cresol red indicator is a ethanol solution of cresol red. The provided method has the advantages of short course, low cost, rapidness, efficiency, accuracy, and good stability, and can be easily mastered.

The invention discloses a cresol red colorimetric method for soil respiration measurement and a measurement device. The measurement device comprises a plastic test tube containing a cresol red reagent and a sealing cover. The plastic test tube and soil to be measured are sealed in the sealing cover, the absorbancy of the cresol red reagent is measured with the colorimetric method under the wavelength of 560 nm after respiration, and soil respiration intensity is measured through a standard curve. By preparing the cresol red reagent from the acid-base indicator cresol red, neutral salt and bicarbonate to absorb CO2 in soil, cultured soil respiration intensity is measured, and field soil respiration intensity can also be measured; strong base such as NaOH with high corrosivity is not needed, the reagent can be used repeatedly, sensitivity and safety performance are high, special or expensive instruments are not needed, the operation method is convenient and quick, and test data are reliable and accurate. The measurement device also has the advantages of being simple in structure, low in price, and convenient and rapid to use and operate.

The invention discloses a thermochromic material, which is a system of hollow mesoporous silica loaded cresol red-boric acid, or a system of amino-modified hollow mesoporous silica loaded cresol red-boric acid. The invention further discloses a preparation method of the thermochromic material and application of the thermochromic coating material in cigarette paper. The thermochromic material disclosed by the invention has a prompting effect on temperature.

The embodiments of the present invention disclose a method for detecting the disinfection liquid residue on the explant surface during tissue culture. The method comprises: washing: washing explant surface to obtain a washed explant; disinfecting: soaking the washed explant in 70% alcohol and a 5-10% NaClO solution; disinfecting liquid residue removing: rinsing the disinfected explant by using sterile water; and detecting: adding a cresol red indicator to the last sterile water washing waste liquid in a dropwise manner, and observing the color of the waste liquid to determine the residue. According to the present invention, the washing agent is rationally used in the explant disinfecting method , and the explant is subjected to a series of the disinfecting operations and the detection step by matching the cleaning, detecting and observing technology; and the detection method is simple and easy to perform, does not cause any added damage to the explant, can accurately determine the disinfecting liquid residue removing degree, and improves the production efficiency under the premise of the ensuring of the explant survival rate.

The invention provides a soybean urease activity detecting tube which comprises a urea cresol red sodium salt extracting fluid tube, a sample quantifying tube and a bottle cap which are sequentially connected. The detecting tube has simple structure and stable sampling quality and avoids large fluctuation. The invention further provides a method for detecting soybean urease activity. The method comprises the steps of adding a soybean meal sample into the sample quantifying tube, connecting the sample quantifying tube with the top of the urea cresol red sodium salt extracting fluid tube, shocking, recording color change time and calculating a soybean urease activity value of the soybean meal sample according to a corresponding fitted equation. The detecting method has simple steps, short consumed time in a detecting process and not high requirements for skilled degree of operators and equipment; furthermore, detecting results are identical with detection results of a national-standard method.

The invention discloses a soybean milk ripeness degree rapid detection card and a detection method thereof, and relates to the field of soybean milk ripeness degree detection. The soybean milk ripeness degree rapid detection card comprises a bottom plate, wherein a sample pad, a reagent pad, a color development pad and a water absorption pad are sequentially pasted on the bottom plate from left to right, the sample pad is made of polyester fibers, the sample pad is soaked in a phosphate buffer solution and dried for later use, the reagent pad is impregnated with a urea solution and dried for later use, and cresol red with a mass percent of 0.5% is dipped on the color development pad. According to the invention, the soybean milk ripeness degree rapid detection card has filtering and impurity removing functions, wherein pigments and small particles in soybean milk liquid to be detected can be filtered and adsorbed, so that the covering effect of the background color of the soybean milk on the water absorbing paper is reduced, and the soybean milk ripeness degree rapid detection card is suitable for pure soybean milk, five-cereal soybean milk, black soybean milk and other soybean milk with various colors in the market added with red dates, Chinese wolfberry fruits and the like; and the method is easy to operate, high in sensitivity, applicable in technology, low in cost, safe to operate, free of flammable and explosive solutions, non-toxic, harmless and free of organic reagents.

The invention specifically discloses a preparation process for cresol red, which belongs to the field of synthetic technology of indicators. The preparation process comprises the following steps: 1, with o-benzoyl sulfonic anhydride, o-benzoyl and zinc chloride anhydrous in a mass ratio of 1: 1-2: 1-2.5 as raw materials, controlling reaction temperature to be 100 to 110 DEG C and reaction time to be 3 to 4 h and adding hydrochloric acid and water with slow stirring so as to prepare a tiny particle; 2, subjecting the tiny particle prepared in the step 1 to filtration and deposition in warm water with a temperature of 45 to 60 DEG C; and 3, successively subjecting a sediment of filtration obtained in step 2 to heating, drying and cooling, adding diethyl ether and benzene, carrying out slow stirring for 15 to 30 m and carrying out filtration and drying so as to finally prepare cresol red. The preparation process for cresol red provided by the invention overcomes the problems of low efficiency and low purity of conventional processing and preparation technology, meets demands of middle and small-sized enterprises for synthesis technology and can rapidly and easily process and prepare cresol red with high purity.

The invention relates to a method for preparing semixylenol orange by one-step operation, including the following steps: (1) cresol red, iminodiacetic acid disodium salt and glacial acetic acid are mixed and stirred evenly, the mixture is heated till full dissolving at the temperature of 60 to 65 DEG C and then is cooled to 25 to 30 DEG C to obtain a cooled mixed liquid; (2) 37 percent of formaldehyde solution is added into the cooled mixture which is rested for 7 to 8 hours and then is constantly stirred for 7 to 8 hours at the temperature of 60 to 65 DEG C; (3) the unwanted solution is removed by decompression and the remaining black thick matter is poured into an evaporation tank to be steamed to be dry at the steaming temperature of 70 to 80 DEG C to form crystals; and (4) the crystals are crushed to obtain the orange-red solid-semixylenol orange finished products. The one-step synthesizing method adopted by the invention greatly enhances the conversion rate; the yield is higher; the sensitivity reaches the standard of similar products; the method is suitable for the large-scale industrial production.

The invention discloses a chloroethylene detection reagent and a preparation method thereof, and belongs to the technical field of gas detection. The detection reagent comprises the following components in parts by weight: 15-25 parts of phosphomolybdic acid, 5-10 parts of dimethyl yellow, 2-9 parts of sodium sulfate, 3-7 parts of cresol red, 2-5 parts of tricresyl phosphate, 2-6 parts of vulcanized zinc dimethyl dithiocarbamate, 10-25 parts of dimethylacetamide and 15-25 parts of deionized water. The preparation method comprises the steps: (1) preparing a pre-mixed liquid; (2) obtaining a clear mixed liquid; and (3) standing under a vacuum condition for 12-48h, and sealing so as to obtain the chloroethylene detection reagent. The chloroethylene detection reagent provided by the invention is easy to operate, high in sensitivity and high in measurement accuracy and can be used for effectively detecting the content of chloroethylene in air.

The invention discloses an infiltration water and inorganic solute global distribution test method under a micro-spraying irrigation condition. The method comprises the following steps: arranging a test area, and measuring physical and hydrodynamic parameter properties of soil; dissolving an inorganic solute solution in water to serve as a tracer agent, and carrying out a micro-spraying experimentin a test area; preparing a cresol red-methylene blue mixed color developing solution; after 24-48 hours, digging out an area which is not sprayed, forming a section layer by layer along the axis direction, and measuring the soil moisture content at different positions and different depths and the concentration distribution of a color developing area; determining the soil moisture content variation of the color developing area according to the measured soil moisture content, and determining the satisfaction degree of spray irrigation water and inorganic solute redistribution on irrigation requirements according to the variation of the moisture content and the concentration distribution of the color developing area at different depth positions. According to the method, the micro-spraying irrigation efficiency can be systematically evaluated by measuring the redistribution of water and inorganic solutes under the condition of micro-spraying irrigation; and a basis is provided for improving the micro-spraying irrigation level and realizing precise agricultural water.