The invention discloses a cultivation method of leaf vegetable with low nitrate content. The method comprises the following steps: A, land preparation: turning over the soil, ditching and ridging; B, fertilization: applying chemical nitrogenous fertilizer potassium, wherein 100 kg of N, 50 kg of P and 50 kg of K are applied to each hectare; C, seeding: seeding the sprouted vegetable seeds into the fertilized soil after the land preparation; D, watering: watering and covering the soil with a thin film or sunshade net after the seeding; E, spraying plant physiological regulator: spraying the prepared plant physiological regulator in the growth period of vegetable, once every week and three in total; F, prevention and control of disease, pest and grass: spraying liquid medicine for preventing disease and killing pest, and weeding in the vegetable plot; and G, harvesting: harvesting the ripe crops. The method is easy to implement and convenient to operate, and has low cost; the plant physiological regulator has good effect and is convenient to use and widely applicable; and moreover, the method is widely applicable to the planting of leaf vegetable in the same type of vegetable plot soil, reduces nitrate content of leaf vegetable, improves the vegetable quality and the soil quality, and prevents widespread pollution caused by excessive soil nutrient.