The invention discloses a pig tonsil living body sampler operated by a single person. The pig tonsil living body sampler comprises a main pipe, a head outer sleeve, a sampling knife head assembly anda sampling auxiliary assembly, the sampling knife head assembly comprises a positioning plate, a scissor-type lever knife handle, a fixed knife head, a permanent magnet, an electromagnet and a reset spring, the sampling auxiliary assembly comprises a lighting bulb, a camera, a display screen, a power supply and a control switch, the lighting bulb and the camera are installed on the positioning plate, the display screen is installed on the main pipe, the power supply is arranged inside the main pipe, a detachable sealing structure is arranged at the lower end of the main pipe, the head outer sleeve is connected to the head of the main pipe in a sleeving mode, a reserved hole corresponding to the positioning plate is formed in the upper end of the head outer sleeve as well, and the outer wall of the head outer sleeve is set to be in a serrated shape for food pasting. The pig tonsil living body sampler is more flexible and more convenient than an existing sampler, a pig is not hurt exceptthe tonsil part in the operation process, and the threat to a sampling person due to excessive fright is also avoided.