Method for culturing cobble alga in large scale outdoor, and its processing method

A large-area, pebble algae technology, applied in the direction of single-cell algae, etc., can solve the problems of unrealized outdoor large-area cultivation, and achieve the effect of shortening the expansion cycle and saving production time

Inactive Publication Date: 2008-03-19
ENG TECH INST CO LTD OF CNSIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the culture density of this algae in the past was only 500,000 to 600,000 / ml, and it was only limited to laboratory cultivation, and outdoor large-scale cultivation has not been realized.

Method used

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  • Method for culturing cobble alga in large scale outdoor, and its processing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Put the sterilized seawater and lake water into the first-level breeding shallow water channel photobioreaction reactor, adjust the water body to make NaCL 2.5% to 3.0%, pH to 7 to 9, and nutrient concentration to 0.4 mmol / L KNO 3 , 0.05 mmol / L K 2 HPO 4 , 0.5 mmol / L NaHCO 3 , 10 micromole / liter Fe-EDTA. Inoculate at a density of 100,000 / ml, and breed for 9 to 12 days; enter the second-level breeding stage, place the above-mentioned cultivation culture solution in the shallow waterway culture pond in the glass greenhouse, use natural light sources for cultivation, and inoculate and cultivate from the above-mentioned grade-one cultivation pond liquid, the inoculation density is 100,000 / ml, and it is cultured for 9 to 12 days; when it enters the third-level breeding stage, the above-mentioned second-level seed expansion liquid is transferred to the above-mentioned culture medium in the plastic greenhouse according to the density of 100,000 / ml, and it is cultivated for 9...

Embodiment 2

[0025] According to the steps described in Example 1, the conditions for using the culture medium are: the temperature is 25°C, the pH value is 7-9, and the nutrient concentration is 0.3 mmol / L KNO 3 , 0.03 mmol / L K 2 HPO 4 , 0.2 mmol / L NaHCO 3 , 2 micromole / liter Fe-EDTA.

Embodiment 3

[0027] According to the steps described in Example 1, the conditions for using the culture medium are: the temperature is 15°C, the pH value is 7-9, and the nutrient concentration is 0.8 mmol / L KNO 3 , 0.08 mmol / L K 2 HPO 4 , 1 mmol / L NaHCO 3 , 15 micromole / liter Fe-EDTA.

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Abstract

The invention discloses a rapid outdoor large scale cultivating and processing method for boulder algae. The invention uses thin waterway type optical biology reactor to take elementary expanding growing, taking relaying expanding growing in greenhouse and plastic tunnel to realize outdoor large scale cultivating. The algae liquid could be processed into algae power. The invention improves high density and excellent seed source and saves producing time.

Description

technical field [0001] The present invention relates to the field of cultivation and processing methods of algae, more specifically, relates to a method for high-density cultivation, outdoor large-area cultivation and processing of marine light-energy autotrophic single-celled algae, pebble algae. Background technique [0002] Pebble algae are generally distributed in cold water sea areas with relatively low temperature, and its optimum growth temperature is between 15 and 25°C. Cyclolithia has no cell wall, but it can form a layer of coccolithophores outside the cell membrane during growth. The ball stones are disc-shaped, about lum in diameter, and about 200 in number. The outer layer of the ball stone is CaCO3 crystal, the middle is protein and acidic polysaccharide, and there are a small amount of elements such as Fe, Mn and Si. Due to the complex crystal structure of CaCO3, the specific surface area is very large, which is several times that of the current common calc...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N1/12
Inventor张俊杰崔志强吕本松高清潭王富平刘洪岩
OwnerENG TECH INST CO LTD OF CNSIC