Fast method of extracting microsporidian DNA

A microsporidian and rapid technology, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of long operation time, complicated extraction steps, and restrictions on large-scale application, and achieve a wide range of applications, Simple operation effect

Inactive Publication Date: 2008-07-16
SOUTH CHINA AGRI UNIV
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  • Abstract
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Problems solved by technology

However, these methods generally have complex extraction steps and long operation time (generally 4-7 hours), which limits their large-scale application in actual production.

Method used

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  • Fast method of extracting microsporidian DNA

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Experimental program
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Effect test

Embodiment 1

[0014] PCR Molecular Detection of Microsporidia Using DNA Extracted by Boiling Method and Other Two Methods (Conventional Method and CTAB Method) as Templates

[0015] With honeybee Microsporidium, Spodoptera litura, Bombyx mori Microsporidium spores, silkworm moth tissue grinding fluid infected with Bombyx mori Microsporidium spores and diamondback moth Microsporidium spore suspension as materials, respectively through the boiling method of the present invention , the reported conventional method (Terry R.S.; Smith J.E.; Bouchon D.; Rigaud T.; Duncanson P.; Sharpe R.G.; DunnA.M., Ultrastructural characterization and molecular taxonomic identification of Nosema granulosis n.sp., a transovarially transmittedfeminising( TTF) microsporidium, Journal of Eukaryotic Microbiology, 1999, 46(5): 492-499); Cao Guangli, Zhang Zhifang, Gong Chengliang, etc. Preparation method of silkworm microspore spore DNA, Sericulture Science, 1995, 21(4): 260) and CTAB method (Liu Jiping, Cao Yang, Sm...

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Abstract

The present invention relates to method of extracting microsporidian DNA, and belongs to the field of microsporidian molecular diagnosis and identification technology. Microsporidian DNA is extracted by using alkali buffering schizolysis liquid containing potassium ion and through a boiling process. The method of the present invention is simple in operation, fast and wide in applicable microsporidian range, ad can prepare enough neucleic acid.

Description

technical field [0001] The invention relates to a method for extracting microsporidia DNA, which belongs to the technical field of microsporidia molecular diagnosis and identification. Background technique [0002] Microsporidia are unicellular protozoa without mitochondria and parasitize intracellularly. Since the discovery of Nosema bombycis, which caused devastating diseases of silkworms, in the last century, Nosema bombycis parasitism has been found in many eukaryotes. Because of its close relationship with the health of animals and humans, it has attracted extensive research interest. The study of molecular biology of microsporidia is of great biological and economic significance. On the one hand, it is beneficial to enrich the biological population and provide better resources for biological control; at the same time, it is helpful to explore the practical technology of detecting silkworm infection with microspores, improve the quality of silkworm egg quarantine, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/06C07H21/04
Inventor 刘吉平曾玲曹阳
Owner SOUTH CHINA AGRI UNIV
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