General purpose primer for cloning bacteria gyrase-gyrB gene

A general primer, gyrase technology, applied in sugar derivatives, organic chemistry, DNA/RNA fragments, etc., can solve the problem of limited amount of genetic information, and achieve the effect of strong specificity and sensitivity

Inactive Publication Date: 2008-11-05
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0008] The purpose of the present invention is to provide a new universal primer of gyrB gene, which solves the problem that the amount of genetic information contained in existing gyrB gene primers is limited

Method used

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  • General purpose primer for cloning bacteria gyrase-gyrB gene
  • General purpose primer for cloning bacteria gyrase-gyrB gene
  • General purpose primer for cloning bacteria gyrase-gyrB gene

Examples

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Embodiment 2

[0042] Through these two primers, acidophilus ferrooxidans (ATCC 23270 and DQ062115 (the 16S rDNA sequence accession number of this strain is DQ672266) of Acidithiobacillus), Leptospirillum ) (the 16S rDNA sequence accession numbers of these strains are: DQ343299, EF025341, EF025340, EF025337, DQ451017 and EF025342) and Leptospira ferrooxidans (ATCC 53992), Sphingosine Sphingomonas XJ-1 of Sphingomonas (the 16S rDNA sequence accession number of this strain is DQ672266) and a strain of Acidiphilum (ATCC 15859), a total of 4 genera and 11 strains were amplified from the genomic DNA gyrB gene (shown in Table 2).

[0043] Table 2 utilizes the gyrB gene of the strain amplified by the primers of the present invention

[0044]

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Abstract

The invention discloses a general primer of cloned bacterium spiral-accelerating enzyme-gyrB gene, whose nucleic sequence of upstream primer is 5'-CC(ATGC)GG(ATGC)ATG TA(CT) AT(ATC)GG-3' and the nucleic sequence of downstream primer is 5'-CAT (CT) TC (ATGC) CC (ATGC) A (AG) (ATGC) CC (CT) TT (AG) (AT) A (ATGC) C (GT) (CT) TG-3'), wherein the whole sequence of PCR product approximates to the whole sequence of gyrB gene, which possesses stronger specificity and sensitivity.

Description

technical field [0001] The invention relates to the field of microbial phylogenetic molecular markers, in particular to a universal primer for bacterial gyrase-gyrB gene. Background technique [0002] In modern microbial taxonomy, 16S rDNA has been widely used to study the phylogenetic status of bacteria, but a large number of studies have shown that for strains with close kinship, simply relying on 16S rDNA homology between strains to determine species is not enough. The gyrB gene is a gene encoding the B subunit of DNA gyrase (a prokaryotic type II topoisomerase). As a new molecular marker, the gene has been widely used in bacterial and fungal species-level identification and natural Detection of microorganisms in ambient conditions. Studies on the systematic taxonomic identification of Aeromonas, Pseudomonas and Shewanella have proved that the molecular classification index of the gyrB gene has the following characteristics for the identification of bacterial species wi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H21/04C12N15/52
Inventor 邱冠周夏金兰张成桂高健丁建南
Owner CENT SOUTH UNIV
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