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Enzyme combining stabilizer

A composite stabilizer, lactate dehydrogenase technology, applied in the direction of enzyme stabilization, peptide/protein components, medical preparations containing active ingredients, etc. The effect of strong stabilization

Active Publication Date: 2008-11-19
SHANGHAI FOSUN LONG MARCH MEDICAL SCI CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the quality of clinical diagnostic reagents at home and abroad is uneven, and there are great differences in the specificity and sensitivity of the reagents.
As an important performance index of diagnostic reagents-stability is particularly obvious. The test data of the kit at the beginning of production and the end of the validity period are often very different. For example, when the XX enzyme kit was tested on December 9, 2003, the measured value was When the same sample was tested on April 27, 2004, the measured value was 164U / L, but it was only 91U / L when tested on September 30, 2004. The measured value dropped significantly, and these results will seriously affect clinical diagnosis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0020] Bovine serum albumin 2 grams

[0021] EGTA 4 grams

[0022] 1,2-Dithiothreitol 25g

[0023] Potassium Gluconate 30g

[0024] Sodium chloride 20 grams

[0025] proclin 300 1 g,

[0026] Magnesium acetate 8 g

[0027] Take 5 ml of the above compound enzyme stabilizer and add it to 95 ml, respectively containing lactate dehydrogenase (A liquid), sarcosine oxidase (B liquid), urease (C liquid), creatinase (D liquid), creatine hydrolase (Liquid E), catalase (Liquid F), cholesterol oxidase (Liquid G), cholesterol esterase (Liquid H), peroxidase (Liquid I) in the enzyme preparation, after 5 days in a 37-degree oven, Detect the residual enzyme activity respectively (with the enzyme preparation without compound enzyme stabilizer as the control) (see figure 1 )

example 2

[0029] Bovine serum albumin 2 grams

[0030] EDTA 10 g,

[0031] 1 gram of sucrose

[0032] Potassium Gluconate 10g

[0033] Sodium chloride 60g

[0034] proclin 300 1 g

[0035] Magnesium Chloride 24g

[0036] Take 5 ml of the above compound enzyme stabilizer and add it to 95 ml, respectively containing lactate dehydrogenase (A liquid), sarcosine oxidase (B liquid), urease (C liquid), creatinase (D liquid), creatine hydrolase (Liquid E), catalase (Liquid F), cholesterol oxidase (Liquid G), cholesterol esterase (Liquid H), and peroxidase (Liquid I) in the enzyme preparation, put in the refrigerator at 2-8 degrees for 12 After one month, detect residual enzyme activity respectively (with the enzyme preparation that does not add composite enzyme stabilizer as contrast)

[0037] Table 1: Residual Enzyme Activity Control

[0038]

example 3

[0040] Bovine serum albumin 2 grams

[0041] EGTA 0.1 g,

[0042] Mannitol 300g

[0043] Potassium Gluconate 183g

[0044] Sodium chloride 100 grams

[0045] proclin 300 1 g

[0046] Magnesium acetate 24g

[0047] Take 5 ml of the above compound enzyme stabilizer and add it to 95 ml, respectively containing lactate dehydrogenase (A liquid), sarcosine oxidase (B liquid), urease (C liquid), creatinase (D liquid), creatine hydrolase (Liquid E), catalase (Liquid F), cholesterol oxidase (Liquid G), cholesterol esterase (Liquid H), and peroxidase (Liquid I) in the enzyme preparation, put in the refrigerator at 2-8 degrees for 12 After one month, detect residual enzyme activity respectively (with the enzyme preparation that does not add composite enzyme stabilizer as contrast)

[0048] Table 2: Residual Enzyme Activity Control

[0049]

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Abstract

The present invention provides a kind of efficient enzyme combining stabilizer with high stabilizing effect on enzymes for clinical diagnosis reagent. The enzyme combining stabilizer consists of bovine serum albumin, EGTA, 1, 2-dithio threitol, potassium gluconate, sodium chloride, proclin300, magnesium acetate, etc. It has powerful stabilizing effect on lactate dehydrogenase, sarcosie oxidase, urease, creatinine enzyme, creatine hydrolase, etc.

Description

Technical field: [0001] The invention belongs to the field of biotechnology. Specifically relates to an enzyme composite stabilizer. Background technique: [0002] With the development of the medical industry, the field of medical clinical diagnosis is gradually developing, various clinical diagnostic techniques have been further improved, and clinical diagnostic reagents have been continuously innovated, which has provided great help for clinical diagnosis. Because it is used for clinical diagnosis, it requires stable quality of diagnostic reagents, which can prevent misdiagnosis caused by reagent quality problems. Therefore, the stability research of clinical diagnostic reagents has been included in the concern of people. However, at present, the quality of clinical diagnostic reagents at home and abroad is uneven, and there are great differences in the specificity and sensitivity of the reagents. As an important performance index of diagnostic reagents-stability is par...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/96A61K38/43
Inventor 孙卫兵
Owner SHANGHAI FOSUN LONG MARCH MEDICAL SCI CO LTD
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