Hot air circulating temperature controlling real-time fluorescent quantitative PCR detector

Abstract

The invention relates to a hot air circulation temperature-controlled real-time fluorescent quantitative PCR detector, which includes an air bath device, a PCR reaction detection chamber located at the lower part of the air bath device, an excitation optical path located outside the detection chamber, and an excitation optical path perpendicular to the excitation optical path. The detection optical path, a signal acquisition and processing system, a communication system and an external computer connected with the communication system are characterized in that it also includes a rotary motion device located at the lower part of the detection cavity. The rotary motion device is provided with at least one temperature detection sensor and a real-time fluorescence correction device, and realizes the circuit communication between the upper part and the lower part when the lower part is fixed and the upper part is rotating through a rotary connector. Because the temperature detection sensor and the sample tube in the present invention rotate on the same disc, the object of accurate temperature control of the sample in the PCR detection process can be achieved. The invention detects the polymerase chain reaction and can improve the accuracy of the detection result.

Description

technical field [0001] The invention relates to a detection device for polymerase chain reaction (PCR), in particular to a hot air circulation temperature-controlled real-time fluorescent quantitative PCR detector. Background technique [0002] In the process of molecular biology research and medical clinical diagnosis, polymerase chain reaction technology is widely used. In recent years, fluorescent quantitative PCR technology, which completes PCR in a closed system and detects the end product of amplification in real time, has gradually been accepted by the industry. And widely used, and then appeared a variety of real-time fluorescent quantitative PCR detection instruments. Some of these instruments use hot air circulation temperature control to provide the temperature of different stages of PCR, while the sample is rotated in the sample reaction chamber for detection. However, the general PCR detector adopting this method basically loads a temperature detector at a fixe...

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Problems solved by technology

However, the general PCR detector adopting this method basically loads a temperature detector at a fixed position in the sample reaction chamber to detect and regulate the temperature in the reaction chamber. At this time, since the temperature detector is fixed at a certain position, and the sample is rotating, so it is inevitable that there will be differences or inhomogeneities between the actual temperature of the rotating sample itself and the temperature detected by the temperature detector at a fixed position in the reaction chamber The problem caused by the inaccurate temperature control of the sample during the PCR detection process

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