Hot air circulating temperature controlling real-time fluorescent quantitative PCR detector

Abstract

The invention relates to a real-time fluorescent quantitative PCR detector based on hot air circular temperature controlling. It contains a wind-bath device, a PCR reaction testing cavity bellow wind-bath device, an exciting optical path outside testing cavity, a detecting optical path vertical with exciting optical path, a signal collecting and processing system, a communication system, an external computer and a rotary moving device bellow testing cavity. At least one temperature detecting sensor and one real-time fluorescent corrector are set on rotary moving device which realizes circuit connectivity between upper and lower parts under state of lower part fixing and upper part rotating by using a rotary connector. Because that the temperature detecting sensor and sample tubes are rotates on the same disk, the invention can control temperature accurately during PCR detecting. Detect polymerized enzyme chain reaction by using this invention can improve the accuracy of detecting results.

Description

technical field [0001] The invention relates to a detection device for polymerase chain reaction (PCR), in particular to a hot air circulation temperature-controlled real-time fluorescent quantitative PCR detector. Background technique [0002] In the process of molecular biology research and medical clinical diagnosis, polymerase chain reaction technology is widely used. In recent years, fluorescent quantitative PCR technology, which completes PCR in a closed system and detects the end product of amplification in real time, has gradually been accepted by the industry. And widely used, and then appeared a variety of real-time fluorescent quantitative PCR detection instruments. Some of these instruments use hot air circulation temperature control to provide the temperature of different stages of PCR, while the sample is rotated in the sample reaction chamber for detection. However, the general PCR detector adopting this method basically loads a temperature detector at a fixe...

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Problems solved by technology

However, the general PCR detector adopting this method basically loads a temperature detector at a fixed position in the sample reaction chamber to detect and regulate the temperature in the reaction chamber. At this time, since the temperature detector is fixed at a certain position, and the sample is rotating, so it is inevitable that there will be differences or inhomogeneities between the actual temperature of the rotating sample itself and the temperature detected by the temperature detector at a fixed position in the reaction chamber The problem caused by the inaccurate temperature control of the sample during the PCR detection process

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