Method for high-effective xylanase fixation and improving reusing effect of solidified xylanase

Abstract

An efficient method of xylanase immobilization and improving reutilization effect thereof, the immobilization method is: blending Eudragit L-100 and xylanase by a certain proportion, agitating continuously 1.5-3 hours at 25 DEG C to obtain xylanase. After reaction of the xylanase and substrate, adding coagulator, regulating pH value from 3.8 to 4.2 by acetic acid, quiescence 25-40 minutes, centrifugating 30 minutes in condition of 5000xg to finish recovery of immobilized enzyme. The advantages of the invention used to industrialized enzyme method for producing xyloolgosaccharides are: efficient immobilized efficiency for immobilizing enzyme on the carriers; the immobilized enzyme can homogeneous react with substrate to improve enzyme efficiency; using method of improving reutilization of the immobilized enzyme can improve reutilization efficiency of immobilized xylanase using reversible solution material Eudragit L-100remarkbly as carrier.

Description

technical field [0001] The invention relates to a high-efficiency xylanase immobilization method and a method for improving the reutilization effect of the immobilized xylanase. Background technique [0002] Xylooligosaccharide (Xylooligosaccharide), as a functional food additive with high added value and promising market prospect, is one of the functional oligosaccharides being researched and developed at home and abroad. Xylooligosaccharides, also known as xylooligosaccharides, are composed of 2 to 7 D-xyloses connected by β-1,4-xylosidic bonds, and some of them also contain side chains such as arabinose and glucuronic acid. It is an oligosaccharide produced from natural food fibers (hemicellulose) such as cottonseed hulls, corncobs, and bagasse by saccharification and decomposition with xylanase. In Japan, xylooligosaccharides are considered to be one of the most promising functional oligosaccharides and have been widely used. [0003] In addition to good physical and c...

AI Technical Summary

Problems solved by technology

Therefore, using reversible soluble materials as carriers to immobilize xylanase has broad industrial application prospects, but when using reversible soluble materials as immobilized xylanases, after adjusting the pH value, the immobilized enzyme will It cannot be completely separated under the condition of centrifugal force, and the binding mode of immobilization and substrate is non-covalent bond adsorption mode, and the combination between them is not tight enough, resulting in low reutilization rate of the immobilized enzyme.