Method for rapid inspecting animalcule in liquid

A detection method and microbial technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, measuring devices, etc., and can solve the problems of product quality without real-time guiding significance and long cultivation time

Inactive Publication Date: 2008-01-02
赵化平 +2
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional microbiological inspection method has no real-time guiding significance for product quality control due to the long incubation time

Method used

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Experimental program
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Embodiment Construction

[0007] The specific implementation method of realizing the present invention is: the first step carries out weak acidification to the liquid sample to be tested, denatures the proteinaceous material wherein, makes its pH value drop below the isoelectric point of casein; The second step Centrifuge the detected liquid samples to remove proteins, fats and impurities, while improving the detection accuracy; the third step is to use the optical microscope system to perform microscopic imaging of the detected liquid samples; the fourth step is to use the system The special computer image recognition technology processes the microscopic images of microorganisms, stores and displays relevant data, and can complete the rapid detection of microorganisms in liquids.

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Abstract

The invention relates to microbios quick test method in liquid which adopts acid-treatment, centrifugal separation, microscopic imaging, computer pattern recognition technique and programme. The fundamental difference between the invention and other microbios test method is: coloring agent is not used during course of test, without bacterial cultivation, liquid sample book detected is acidified and centrifuge treated. The create mode of execution is: firstly liquid sample book detected is acidified, so protein can be denaturation processed; thereinto the liquid sample book is centrifugal separated to clear protein, fat and impurity; then the liquid sample book is imaged using optical microscopic imaging system; and adopting computer pattern recognition technique to process the microscopic image of microbios, so purpose of detecting quantity of microbios within 2-3 minutes without coloring agent and train can be realized.

Description

technical field [0001] The invention relates to a rapid detection method for microorganisms in liquid, which does not need to culture and stain bacteria, and adopts acidification and centrifugation treatment, microscopic imaging, computer image recognition technology and program for tested liquid samples. Background technique [0002] Rapid microbial detection is one of the technical means to control food quality and safety. Common detection methods for microbial detection include culture and staining. Both the culture method and the staining method are operated completely by hand, and the operation steps are relatively complicated. The culture method requires more than 48 hours of culture time, and the detection efficiency is low; the chemical reagents used in the staining method are prone to environmental pollution. The invention adopts the rapid detection method of microorganisms in the liquid which is subjected to acidification treatment, centrifugal separation, micros...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84G01N15/00C12Q1/02
Inventor 赵化平刘刚赵秀忠
Owner 赵化平
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