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Monoclonal antibody for resisting bovine prion protein and application thereof

A technology of monoclonal antibody and prion protein, applied in the direction of anti-viral immunoglobulin, anti-animal/human immunoglobulin, antibody, etc., can solve the problems of early diagnosis of disease and achieve the effect of maintaining specificity and small molecular weight

Inactive Publication Date: 2008-02-20
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Brain histopathological examination is the gold standard for the diagnosis of the disease, but it cannot be diagnosed in the early stage of the disease

Method used

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  • Monoclonal antibody for resisting bovine prion protein and application thereof
  • Monoclonal antibody for resisting bovine prion protein and application thereof
  • Monoclonal antibody for resisting bovine prion protein and application thereof

Examples

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Embodiment 1

[0019] According to the purpose and content of the present invention, the embodiment is divided into three parts to complete. Example 1: Preparation of monoclonal antibody against bovine PrP27-30 and identification of its characteristics; Example 2: Cloning, sequencing and analysis of genes encoding light and heavy chain variable regions of mab-PrP102-6; Example 3: mab-PrP102- 6 and the application of the gene encoding the variable regions of the light and heavy chains. The experimental methods in the following examples are conventional methods unless otherwise specified.

[0020] Example Preparation of monoclonal antibody mab-PrP102-6 against bovine PrP27-30 and identification of its characteristics

[0021] 1. E.Coli expression and expression product purification of bovine PrP27-30

[0022] 1.1 Construction of bovine PrP27-30 recombinant expression plasmid p30a-boPrP27-30

[0023] 1.1.1 PCR amplification of the gene encoding bovine PrP27-30

[0024] The template for PCR...

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Abstract

The invention relates to a monoclonal antibody prepared with genetic engineering and the preparation method and usage thereof. Exactly speaking, the invention relates to monoclonal antibody mab-PrP102-6 of bovine prion protein PrP27-30 and .

Description

technical field [0001] The present invention relates to adopting genetic engineering to prepare a kind of monoclonal antibody (hereinafter referred to as monoclonal antibody) and its preparation method and application, specifically the anti-bovine prion protein PrP27-30 monoclonal antibody mab-PrP102-6 prepared by genetic engineering, As well as the preparation method and application of the monoclonal antibody. Background technique [0002] Mad cow disease is a progressive and fatal zoonotic infectious disease. The International Office of Epizootics has listed mad cow disease as a class A infectious disease. my country has also included it as one of the routine inspection infectious diseases in the quarantine inspection process of imported and exported cattle and cattle products. And every year Appropriate general inspections are also carried out on local cattle. In recent years, mad cow disease has caused serious economic losses to the animal husbandry in many countries, es...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C07K16/08A61K39/395A61P31/12A61P25/00
Inventor 张杰刘永生孙德惠陈豪泰
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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