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Ophiopogon root external sperm nutrient solution and preparation method and application thereof

A technology of sperm nutrient solution and Ophiopogon japonicus, applied in the field of sperm nutrient solution, can solve the problem of high price, and achieve the effects of reducing price, prolonging in vitro survival time, and improving sperm motility rate.

Inactive Publication Date: 2011-06-08
SHANDONG PROVINCIAL FAMILY PLANNING INST OF SCI & TECH
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  • Application Information

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Problems solved by technology

At present, the nutrient solutions used in our country are all imported products, which are expensive, and these nutrient solutions generally contain chemical drug ingredients

Method used

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Embodiment

[0023] The Ophiopogon japonicus in vitro sperm nutrient solution of the present invention is a liquid mixed with polysaccharides extracted from Ophiopogon japonicus raw materials and physiological saline, and the ratio of Ophiopogon japonicus polysaccharides to normal saline is 1 g of Ophiopogon japonicus polysaccharides: 10 ml of normal saline.

[0024] The preparation method of the in vitro sperm nutrient solution of Ophiopogon japonicus is as follows: take Ophiopogon japonicus as raw material, wash it, dry it at 60°C for 2 hours, then pulverize it, put it into 0.8% physiological saline and soak it for 1 hour, and the raw material of Ophiopogon japonicus is The ratio with normal saline is 100g: 900-1150 milliliters, the ratio can be: 100g: 900 milliliters, 100g: 1150 milliliters or 100g: 1100 milliliters etc., the preferred scheme is: the ratio of Ophiopogon japonicus raw material and normal saline is 100g: 1000ml. Then put the soaking solution into a container and heat it a...

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Abstract

The invention discloses an extracorporal sperm nutrient solution of ophiopogon japonicus and a preparing method and application of the nutrient solution, which is a mixture fluid of polysaccharide extracted from ophiopogon japonicus with physiologic saline at proportion of 1g ophiopogon japonicus polysaccharide : 10 ml physiologic saline. The preparing method comprises: taking, washing, drying, and crushing the raw material of physiologic saline, soaking the crushed physiologic saline in physiologic saline, then placing the soaking fluid in a container, heating under 95-105 DEG C and filtering to get the extracted fluid of physiologic saline. The application of the extracorporal sperm nutrient solution of ophiopogon japonicus is like follows: mixing evenly the sperm nutrient solution of ophiopogon japonicus with sperm fluid in proportion 1:1 for culturing and activating sperms. The invention can improve 35-56% activity of sperms, has no harm on sperms, is free from any by effect, cancompletely substitute imported products, and is with much lower price than imported products.

Description

technical field [0001] The invention relates to a sperm nutrient solution, which is an in vitro sperm nutrient solution of Ophiopogon japonicus and its preparation method and application. Background technique [0002] Infertility patients who are infertile due to low sperm motility account for a relatively large proportion of infertility patients. There are many types of oral drugs for the treatment of this type of infertility. It will take several years to take effect orally, which will cause greater economic burden and psychological pressure to the patient, and is prone to various discomforts, which has a certain impact on the health of the patient. For this reason, in recent years, international studies have begun to use sperm nutrient solution to cultivate the vitality of the patient's sperm in vitro, and finally achieve the purpose of enhancing sperm vitality. This nutrient solution that my country uses at present is all imported products, and is expensive, and general...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/08A61K35/52A61P15/08C12N5/076
Inventor 邱毅王苏梅施红王磊光
Owner SHANDONG PROVINCIAL FAMILY PLANNING INST OF SCI & TECH
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