Culture solution for epatocyte scale culture

Abstract

The invention relates to a culture solution for epatocyte scale culture. The culture solution comprises culture medium and protecting agent, wherein the protecting agent comprises the following components with corresponding concentrations in the culture solution as follows: 40-100 mg / L of radix salviae miltiorrhizae extact, 40-100 mg / L of astragalus extract, 10-30 mmol / L of fructose, 10-50mg / L of anti-oxidant and 10<-8>-10<-6> mmol / L of insulin. The culture solution can adopt various mechanisms to effectively relieve the damage brought by various factors to hepatocytes in the process of epatocyte scale culture and inhibit the occurrence of hepatocellular apoptosis, thereby achieving the aims of further improving the density and function status of the epatocyte scale culture and effectively prolonging the time for the in-vitro survival of the hepatocytes.

Description

technical field [0001] The invention relates to a culture fluid for cell culture, in particular to a culture fluid for large-scale culture of hepatocytes. Background technique [0002] In the 1980s, with the maturity of hepatocyte isolation and culture technology and the development of biomedical engineering and other technologies, bioartificial livers based on cultured hepatocytes were gradually developed. People have always hoped to adopt this method of in vitro liver support. To replace the failed liver function of patients and create conditions for them to wait for liver transplantation or their own liver regeneration. At present, it is believed that at least 10 10 Cells of the above order of magnitude, and under the condition of ensuring the cell activity, the more the number of hepatocytes, the better the support and treatment effect. Therefore, the large-scale culture technology of hepatocytes in vitro has become the core technology of the development of bioartificia...

AI Technical Summary

Problems solved by technology

[0008] 2. Relatively insufficient oxygen supply

This lack of oxygen can not only directly cause cell damage and affect the growth rate of cells, but also promote the anaerobic glycolysis of glucose in the medium, increase the production of lactic acid, make the pH value of the medium drop sharply, and affect the liver. Cells cause further damage

[0010] 3. Mechanical damage

However, under the action of many forces in the cell culture process (such as turbulent flow and shear force generated by air bubbles, and collision between microcarriers), cells attached to microcarriers will be damaged, inhibit cell growth, and cause cell death. die

Images