Reagent case for lung carcinoma susceptibility detection through SNPs locus of CYP1A1 gene and ERCC2 gene
A kit and susceptibility technology, applied in the field of kits for detecting susceptibility to lung cancer, can solve problems such as weakening of transcription factor complex performance, decline of repair ability, decline of transcription factor, etc.
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Embodiment 1
[0027] Example 1. Use of detection kits
[0028] Step 1: Extraction of DNA template
[0029] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.
[0030] Step 2: Real-time quantitative PCR reaction
[0031] Use a fluorescent quantitative PCR detection kit that can detect susceptibility to lung cancer, which contains the following primer pairs and fluorescent probes:
[0032] Sense primer 1: 5'-GTGTTAAGTGAGAAGGTGAT-3' (SEQ ID NO: 1) with a Tm value of 56°C
[0033] Antisense primer 1: 5'-AGGATAGCCAGGAAGAGAAA-3' (SEQ ID NO: 2) with a Tm value of 58°C
[0034] Sense primer 2: 5'-ATCAAAGAGACAGACGAGCA-3' (SEQ ID NO: 3) with a Tm value of 58°C
[0035] Antisense primer 2: 5'-TCAGGAAGCCCAGGAAAT-3' (SEQ ID NO: 4) with a Tm value of 54°C
[0036] Fluorescent probe 1: 5'-TATCGGTGAGACCGTTGCCC-3' (SEQ ID NO: 5) with a Tm value of 64°C
[0037] Fluorescent probe 2: 5'-TGCCCAACGAAGTGCTGCAG-3' (SEQ ID NO: 6) with a Tm value of 64°C
[0038] Sense primer 1, ant...
Embodiment 2
[0043] Example 2. Services that guide people to proactively prevent lung cancer
[0044] At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.
[0045] Step 1: DNA Extraction
[0046] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.
[0047] Step 2: Genotyping Assays
[0048] Using the kit provided by the present invention, the SNP site rs1048943 on the CYP1A1 gene and the SNP site rs1799793 on the ERCC2 gene are simultaneously detected by fluorescent quantitative PCR to determine the genotypes of the two SNPs sites.
[0049] Step 3: Instruct people to be proactive in preventing lung cancer
[0050] Through the analysis of the SNPs genotype of the tested sub...
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