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Reagent kit for detecting pulmonary cancer susceptibility with CYP1A1 gene and ERCC2 gene

A kit and susceptibility technology, applied in the field of kits for detecting susceptibility to lung cancer, can solve problems such as base excision repair cannot be performed correctly, DNA damage sites cannot be correctly identified, carcinogenic damage accumulation, etc.

Inactive Publication Date: 2008-03-12
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Loss of function of ERCC2 results in the inability of the essential transcription factor complex to correctly recognize sites of DNA damage, thereby preventing base excision repair from proceeding correctly, leading to the accumulation of oncogenic lesions on DNA

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Use of detection kits

[0028] Step 1: Extraction of DNA template

[0029] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0030] Step 2: Real-time quantitative PCR reaction

[0031] Use a fluorescent quantitative PCR detection kit that can detect susceptibility to lung cancer, which contains the following primer pairs and fluorescent probes:

[0032] Sense primer 1: 5'-GTGTTAAGTGAGAAGGTGAT-3' (SEQ ID NO: 1) with a Tm value of 56°C

[0033] Antisense primer 1: 5'-AGGATAGCCAGGAAGAGAAA-3' (SEQ ID NO: 2) with a Tm value of 58°C

[0034] Sense primer 2: 5'-ACTCAGGAGTCACCAGGAA-3' (SEQ ID NO: 3) with a Tm value of 58°C

[0035] Antisense primer 2: 5'-TCTGTTCTTCTGCAGGAGGA-3' (SEQ ID NO: 4) Tm value is 58°C

[0036] Fluorescent probe 1: 5'-TATCGGTGAGACCGTTGCCC-3' (SEQ ID NO: 5) with a Tm value of 64°C

[0037] Fluorescent probe 2: 5'-AATCTGCTCTTATCCTCTGCAGC-3' (SEQ ID NO: 6) with a Tm value of 66°C

[0038] Sense primer 1, antise...

Embodiment 2

[0043] Example 2. Services that guide people to proactively prevent lung cancer

[0044] At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.

[0045] Step 1: DNA Extraction

[0046] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.

[0047] Step 2: Genotyping Assays

[0048] Using the kit provided by the present invention, the SNP site rs1048943 on the CYP1A1 gene and the SNP site rs13181 on the ERCC2 gene are simultaneously detected by fluorescent quantitative PCR to determine the genotypes of the two SNPs sites.

[0049] Step 3: Instruct people to be proactive in preventing lung cancer

[0050] Through the analysis of the SNPs genotype of the tested subje...

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PUM

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Abstract

The present invention discloses a reagent kit to detect lung cancer susceptibility, which comprises a fluorescence probe to synchronously detect specificity tracers and specificity of No. rs1048943 SNP locus on gene CYP1A1 and No. rs13181 SNP locus on gene ERCC2, and a normal assembly to detect fluorescence quantitative PCR and so on. The reagent kit of the present invention synchronously detects and analyzes individual SNPs locus gene carrying type on gene CYP1A1 and ERCC2 to predict susceptibility of the individual to lung cancer.

Description

technical field [0001] The present invention relates to a kit for detecting disease susceptibility, especially a kit for detecting susceptibility to lung cancer. Single nucleotide polymorphism (SNP) site and excision repair complex 2 (Excision Repair Cross-complementing Rodent Repair Deficiency, Complementation Group 2, ERCC2) single nucleotide polymorphism site to predict the individual's risk of lung cancer susceptibility. Background technique [0002] Primary bronchial lung cancer, referred to as lung cancer, is one of the most common malignant tumors in the world and a serious threat to human health and life. Tumor cells originate from the bronchial mucosa or glands, and often have regional lymph node and hematogenous metastasis. In the early stage, respiratory symptoms such as irritating cough and blood in the sputum are often present. The speed of disease progression is related to the biological characteristics of the cells. [0003] For half a century, the morbidity...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C12Q1/68
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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