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Stilbene group compounds as well as grape plant extractive containing the same and extraction method

A technology for plant extracts and compounds, which is applied to medical preparations containing active ingredients, plant raw materials, plant/algae/fungus/moss components, etc. simple effect

Inactive Publication Date: 2008-05-21
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The extraction process of resveratrol in the above-mentioned patent documents is mainly obtained by direct immersion and extraction in organic solvents, followed by high-pressure silica gel column chromatography and other steps, and its separation effect is far inferior to that of macroporous adsorption resin treatment.
[0009] Moreover, there is no report of extracting stilbene compounds from grape plants at present

Method used

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  • Stilbene group compounds as well as grape plant extractive containing the same and extraction method
  • Stilbene group compounds as well as grape plant extractive containing the same and extraction method
  • Stilbene group compounds as well as grape plant extractive containing the same and extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1) Pre-treatment: crush and sieve the raw materials after drying in the shade or in the sun;

[0042] 2) Extraction: Place the processed raw material in purified water and heat it under reflux to extract twice, the volume ratio is 8 times and 5 times that of the raw material respectively, the extraction time ratio is 3:2, the extraction temperature is 50°C, and the extracts are combined , concentrated, centrifuged and filtered to obtain a thick paste;

[0043] 3) After drying the thick paste, the content of resveratrol in the product is 0.66%, and the weight content of grapevine is 0.45%;

Embodiment 2

[0045] 1) Pre-treatment: crush and sieve the raw materials after drying in the shade or in the sun;

[0046] 2) Extraction: Put the treated raw material in 50% methanol and heat it under reflux to extract twice, the volume ratio is 8 times and 5 times that of the raw material respectively, the extraction time ratio is 3:2 respectively, the extraction temperature is 50°C, and the combined The extract is concentrated, centrifuged and filtered to obtain a thick paste, which is set aside;

[0047] 3) Purification: ①Add 1 times the weight of 50% ethanol to the paste-like thick paste, dilute and mix; ②Put the diluted solution through column chromatography, and use D101 resin for adsorption; ③Chromatographic elution: use The ethanol-water system with increasing concentration was eluted as mobile phase chromatography, and the concentrations were 20%, 30%, and 60% respectively; ④ Collect 60% of the eluted part, and after drying, the resveratrol content in the product is 5.6%. The weig...

Embodiment 3

[0050] 1) Pretreatment: pulverize and sieve the dried raw materials;

[0051] 2) Extraction: Put the treated raw material in 70% ethanol and heat it under reflux to extract twice, the volume ratio is 10 times and 7 times that of the raw material respectively, the extraction time ratio is 3:2 respectively, the extraction temperature is 55°C, and the combined The extract is concentrated, centrifuged and filtered to obtain a thick paste, which is set aside;

[0052] 3) Purification: ①Add 2 times the weight of 50% ethanol to the paste-like thick paste, dilute and mix; ②Put the diluted solution through column chromatography, and use D101 resin for adsorption; ③Chromatographic elution: use The ethanol-water system with increasing concentration is used as mobile phase chromatography to elute, and the concentrations are 30%, 60%, and 95% respectively; ④ collect 60% of the eluted part, and after drying, the grapevine content in the product is 15.5%;

[0053] 4) Refining: the collected...

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Abstract

The present invention discloses a stilbene compound which considers 3, 4', 5-trihydroxystilbene as monomer for the polymerization, a grape plant extract containing the stilbene compound and a preparation method of the extract. The extract is dark brown, brown or whitish, white powder with bitter taste and contains resveratrol and / or grape prime and / or a tripolymer of the 3, 4', 5-trihydroxystilbene and / or a tetramer of the 3, 4', 5-trihydroxystilbene, wherein, the weight content of the resveratrol and / or the grape prime is more than 1 percent. The preparation method considers the grape plant as the raw material and water methanol, ethanol or acetone as extracting menstruum for extracting, concentrating, column chromatography, recrystallizing and drying to obtain the grape plant extract containing the stilbene compound. The preparation method technology of the present invention is simple, and grapevines and / or leaves and / or rhizomes are used as the raw material, which is the utilization of the waste from the product of the grape planting industry.

Description

technical field [0001] The invention relates to a stilbene group compound extracted from grape plants and a preparation method thereof. Background technique [0002] Resveratrol (resveratrol), the chemical structure is 3,4', 5-trihydroxystilbene, which is a trans-stilbene (i.e., stilbene) compound, and its structure is as follows: [0003] [0004] Viniferin is formed by the free combination of two molecules of resveratrol, and its structure is as follows: [0005] [0006] The oligomers of resveratrol, grapevine and 3,4',5-trihydroxystilbenes are defined as stilbene compounds, which have various biological activities, such as anti-oxidation, inhibiting the formation and development of tumors, improving microbiological Circulation and the therapeutic effect on shock, etc., have the functions of preventing thrombosis, protecting the liver and scavenging free radicals, etc., and have good effects on degenerative diseases of the elderly such as Parkinson's disease, demen...

Claims

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Application Information

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IPC IPC(8): C07C39/21C07C37/68C07D307/83A61K36/87
Inventor 孙步祥汤凌志王智慧胡厚才易永基
Owner GUILIN NATURAL INGREDIENTS CORP
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