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Rabbit anti-big gerbil immune serum

A technology of immune serum and gerbils, which is applied in the direction of antibodies, anti-infective drugs, measuring devices, etc., can solve the problems of non-specificity of immune cross-reaction, deviation of research results and test results, false positives, etc., and achieves convenient use and easy manufacture , The effect of simple process steps

Inactive Publication Date: 2008-07-02
THE CENT FOR DISEASE CONTROL & PREVENTION OF XINJIANG UYGUR AUTONOMOUS REGION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the above-mentioned preparations are used to detect and study other species, problems such as immune cross-reaction and non-specificity will be caused due to species differences, which will cause deviations in research results and test results, such as false positives or false negatives, etc.

Method used

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Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0006] A kind of rabbit anti-gerbil immune serum, it is made by following two process steps:

[0007] a, the preparation of the IgG antigen of the gerbil serum:

[0008] (1) Collect the blood of the gerbil, leave it to stand until the serum is separated out, and then use a centrifuge to separate the blood of the gerbil at a centrifugal speed of 4000 rpm to obtain the serum of the gerbil;

[0009] (2) Remove the particulate matter in the gerbil serum in step (1) with a centrifuge at a centrifugal speed of 6000 rpm;

[0010] (3) Mix the centrifuged serum with an equal amount of normal saline, add saturated ammonium sulfate solution drop by drop to a concentration of 20%, shake while adding, and then place it in a refrigerator at 4°C for 30 minutes , and then centrifuged at a centrifugal speed of 10,000 rpm for 10 minutes to remove impurities and fibrin in the serum;

[0011] (4) Take the supernatant and add saturated ammonium sulfate to a concentration of 35%, then place it in...

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Abstract

A rabbit anti-Rhombomys opimus immune serum is prepared by the following steps of: (1) preparation of Rhombomys opimus serum IgG antigen by collecting Rhombomys opimus blood, separating Rhombomys opimus serum by centrifugation, adding into saturated ammonium sulfate solution, refrigerating, performing high speed centrifugation to remove impurities and fibrin in the serum, collecting the supernatant, supplementing saturated ammonium sulfate solution, refrigerating, performing high speed centrifugation, removing the supernatant, collecting the sediment that is crude Rhombomys opimus serum IgG, purifying, charging into a dialysis bag, and dialyzing overnight; and (2) preparation of rabbit anti-Rhombomys opimus IgG antibody by selecting experimental rabbit, sucking the Rhombomys opimus IgG antigen, immunizing a rabbit by multi-site hypodermic injection along both sides of the rabbit spine for four times, collecting blood from the experimental rabbit, isolating serum, detecting antibody, collecting blood from carotid artery of the experimental rabbit when the antibody titer reaches 1:32, standing, and centrifuging when serum is separated out to obtain rabbit anti-Rhombomys opimus immune serum. The invention has the advantages of simple process and convenient application.

Description

technical field [0001] The invention relates to a kind of biological product, especially rabbit anti-gerbil immune serum. Background technique [0002] Since R. Claus discovered serological reaction in 1877 and J. Boulder discovered immunological cross-reaction in 1899, the technical method of using the specific immunological reaction of antigen and antibody for disease diagnosis and biological identification has been obtained. It is widely used and developed rapidly, but this technical method needs to develop specific antiserum or specific antibody as a specific diagnostic reagent. At the same time, this preparation can be used as a basic preparation for further immunological detection, such as enzyme labeling, fluorescent labeling, molecular labeling, etc. At present, many domestic and international biological preparation companies have developed more specific immune serum for routine scientific experiments and medical testing, such as goat anti-human immune serum, goat a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531A61K39/395A61P31/00
Inventor 戴翔王信惠张渝疆阿扎提阿布利米提
Owner THE CENT FOR DISEASE CONTROL & PREVENTION OF XINJIANG UYGUR AUTONOMOUS REGION
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