Method for detecting beta-carotene, astaxanthin and starch in haematococcus pluvialis under nitrogen stress

Abstract

The invention discloses a method for detecting beta-carotene, astaxanthin and starch in haematococcus pluvialis under nitrogen stress. The method comprises the following steps: S1, obtaining terahertz and far infrared band spectrograms under a haematococcus pluvialis stress environment by using a Fourier infrared spectrometer; S2, obtaining spectrums of beta-carotene, astaxanthin and starch standard substances by using the Fourier infrared spectrometer, and comparing the spectrums with the light obtained in the step S1 under the haematococcus pluvialis stress environment; S3, acquiring content change information of different substances in haematococcus pluvialis in the nitrogen stress environment multiple rounds to prepare a plurality of standard samples; S4, respectively detecting a spectrogram of the haematococcus pluvialis under nitrogen stress and a spectrogram of a metabolite of the haematococcus pluvialis under nitrogen stress by using the standard samples; and S5, establishing a correlation model based on the absorbance value at the characteristic peak position and the corresponding substance component. According to the invention, the problems that the conventional physical and chemical methods have destructiveness in pretreatment, and the physical and chemical methods are difficult to realize synchronous real-time monitoring of various metabolites are solved.

Description

technical field [0001] The invention relates to the technical field of far-infrared spectroscopy in the terahertz band, in particular to a method for detecting β-carotene, astaxanthin and starch in Haematococcus pluvialis under nitrogen stress. Background technique [0002] Haematococcus pluvialis is a freshwater single-celled green algae belonging to Chlorophyta, Chlorophyceae, Volvox, Haematococcus, and Haematococcus genus. It is the organism with the highest natural astaxanthin content in nature. . The cells can rapidly accumulate the ketocarotenoid astaxanthin (3,3-dihydroxy-β,β-carotene-4,4-dione) under unfavorable environments such as nitrogen deficiency and high light. Natural astaxanthin has strong anti-oxidant properties and can protect microalgae cells from photooxidative damage by eliminating reactive oxygen species. Almost all carotenoids have antioxidant properties derived from conjugated double bonds, but the antioxidant activity of natural astaxanthin is sup...

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Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the purpose of the present invention is to provide a method for detecting β-carotene, astaxanthin and starch in Haematococcus pluvialls under nitrogen stress, which solves the problems that are currently caused by physical and chemical methods. Pretreatment, destructive, simultaneously solves the difficulty of simultaneous real-time monitoring of multiple metabolites by physical and chemical methods

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