Yacon antidiabetic extract and preparation method

An anti-diabetic, extract technology, applied in metabolic diseases, drug combinations, pharmaceutical formulations, etc., can solve the problems of inability to anti-diabetic health food and drug applications, no Yagon extract, reports, etc., to achieve suitable for industrial production, The preparation method is simple and the effect of inhibiting activity is good

Active Publication Date: 2008-08-20
珍奥集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there is no definite report on the anti-diabetic active ingredients in yagon, and no report on the extract of yagon
Therefo

Method used

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  • Yacon antidiabetic extract and preparation method
  • Yacon antidiabetic extract and preparation method
  • Yacon antidiabetic extract and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation of agonist diterpene acid extract, according to the following steps:

[0034] a. Take 1 kg of Yagong leaves, add 40 times of water to heat and extract 3 times, combine the extracts, and concentrate to obtain Yagong leaf concentrated extracts;

[0035] b. Put the concentrated extract of Yagong leaves on a macroporous resin column, first elute with water for 3 column volumes, then with 10% ethanol for 3 column volumes, then with 95% ethanol, and collect the 95% ethanol eluate , concentrated under reduced pressure;

[0036] c. Dry to obtain the Yagongo diterpene acid extract.

[0037] Content detection:

[0038] Adopt high-performance liquid chromatography to measure the content of yagonal diterpene acid in the yagonal diterpene acid extract, the conditions are as follows:

[0039] Chromatographic column: C18 column

[0040] Mobile phase: methanol-water, gradient

[0041] 0-5min: 20-25% methanol;

[0042] 5-15min: 25-35% methanol;

[0043] 15-30min: ...

Embodiment 2

[0053] a. Take 1 kg of Yagong leaves, add 40 times of water to heat and extract 3 times, combine the extracts, and concentrate to obtain Yagong leaf concentrated extracts;

[0054] b. Put the concentrated extract of Yagong leaves on a macroporous resin column, firstly elute with water for 3 column volumes, then use 20% ethanol to elute for 3 column volumes, then use 95% ethanol for elution, and collect 95% ethanol for elution liquid, concentrated under reduced pressure;

[0055] c. After the concentrate is acidified by adding HCl, first use CHCl 3 Extract 3 times, collect CHCl 3 layer, the solvent is recovered by methods such as concentration under reduced pressure;

[0056] d. Dry to obtain the Yagongo diterpene acid extract.

[0057] The detection method and conditions are the same as in Example 1. After testing, the present embodiment contains agonist diterpene acids A, B, C, and D. 50% of the total weight of the product.

Embodiment 3

[0059] a. Take 1 kg of Yagong leaves, add 10 times of 50% alcohol to heat and extract for 3 times, combine the extracts, concentrate until no alcohol smell, and obtain the concentrated extract of Yagong leaves;

[0060]b. Put the concentrated extract of Yagong leaves on a macroporous resin column, first elute with water for 3 column volumes, then with 10% ethanol for 3 column volumes, then with 95% ethanol, and collect 95% ethanol for elution liquid, concentrated under reduced pressure;

[0061] c. Put the concentrated solution on a silica gel column and use CHCl 3 - MeOH gradient elution, collect the corresponding fraction;

[0062] d. Concentrate and refine to obtain the Yagong diterpene acid extract.

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Abstract

The invention discloses a Yacon antidiabetic extraction, which is characterized in that the weight percentage of smallanthus sonchifolius diterpenoid acid with the general formula (I) is 0.1 to 100 percent. The preparation method of the Yacon antidiabetic extraction comprises the following steps: first, using water or low-concentration ethanol to extract Yacon leaf and get Yacon leaf concentrated extractant via concentration; next, using water or/and low-concentration ethanol first and then high-concentration ethanol to elute the macroporous resin column on Yacon leaf concentrated extractant to collect high-concentration ethanol eluate; then, concentrating the high-concentration ethanol eluate via reduced pressure; finally, drying and refining. The extract has the advantages of good antidiabetic function proven in vitro test so as to be used for preparing the antidiabetic medicine and easy preparation method, thus being suitable for industrial production.

Description

technical field [0001] The invention relates to a Yagong plant extract, in particular to a Yagong anti-diabetic extract with a simple preparation method and a high content of anti-diabetic active ingredients, which can be used in the preparation of anti-diabetic health food and medicine and a preparation method thereof. Background technique [0002] Yagong (Smallanthus sonchifolius (Poepp. & Endl.) H. Robinson, formerly known as Polymnia sonchifola), Asteraceae (Asteraceae), tubular flower subfamily (Heliantheae) plant, originally grown in the Andes at an altitude of 880-3500m, extending from Venezuela to the northeast of Argentina. Yagon has been planted all over other continents. It was first planted on a small scale in several states of the United States, and then sold as a special vegetable in supermarkets in New Zealand. In the 1980s, Yagon was introduced from New Zealand to Japan in Asia, and then It has been introduced to Italy, Germany, France, the Czech Republic, So...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61K31/202A61P3/10C07C51/47C07C59/42A61K127/00
Inventor 窦德强邱鹰昆康廷国项征董锋
Owner 珍奥集团股份有限公司
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