36results about How to "High inhibitory activity" patented technology

This invention relates to a Kunitz-type potato trypsin inhibitor mutant, its preparation method, and its applications, belonging to the fields of genetic engineering and protein engineering modification technology. Based on a wild-type potato trypsin inhibitor, this invention constructs the mutant shown in SEQ ID NO.3. This mutant exhibits 5.67 times higher trypsin inhibitory activity than the wild type, and in animal models, it increases cholecystokinin (CCK) secretion levels by 1.94 times and reduces food intake by 44.58%. The Kunitz-type potato trypsin inhibitor mutant provided by this invention can serve as an active ingredient and has significant application potential in biomedicine and food health fields, particularly in regulating appetite and alleviating obesity.