Composite ACE (angiotensin converting enzyme) inhibitory peptide with synergistic effect as well as preparation method and application of composite ACE inhibitory peptide

An inhibitory peptide and efficacy technology, applied in the field of compound ACE inhibitory peptide and its preparation, can solve the problems of low nutritional value, increase ACE inhibitory activity, and effect difference, and achieve the improvement of utilization rate, ACE inhibitory activity, good absorption and efficiency. The effect of using

Pending Publication Date: 2022-04-05
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although active peptides from a single source have a certain antihypertensive effect, their nutritional value may be low. Combining active peptides from different biological sources may not only improve ACE inhibitory activity, but also have good nutritional value
However, due to the interaction between the efficacy of polypeptides, the effects of polypeptides from different food protein sources are significantly different after compounding. It is necessary to provide a compound ACE inhibitory peptide to increase the ACE inhibitory activity.

Method used

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  • Composite ACE (angiotensin converting enzyme) inhibitory peptide with synergistic effect as well as preparation method and application of composite ACE inhibitory peptide
  • Composite ACE (angiotensin converting enzyme) inhibitory peptide with synergistic effect as well as preparation method and application of composite ACE inhibitory peptide
  • Composite ACE (angiotensin converting enzyme) inhibitory peptide with synergistic effect as well as preparation method and application of composite ACE inhibitory peptide

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preparation example Construction

[0037] In the present invention, the preparation method of the pea peptide preferably includes: enzymatically hydrolyzing the pea protein and then separating to obtain the pea peptide. The time for the enzymatic hydrolysis of pea protein in the present invention is preferably 4-6 hours. When enzymatically hydrolyzing pea protein in the present invention, the mass ratio of enzyme to pea protein is preferably 1.5-3:100. In the embodiment of the present invention, the mass ratio of enzyme to pea protein can be selected within the range of 1.5 to 3:100, specifically 1.5:100, 1.8:100, 2:100, 2.2:100, 2.5:100 , 2.8:100 or 3:100 etc. In the specific implementation process of the present invention, when pea protein is enzymatically hydrolyzed by enzyme, the amount of enzyme is calculated according to the mass of pea protein raw material. The present invention has no strict requirements on the source of pea protein, and it can be prepared by itself or purchased. During the specific ...

Embodiment 1

[0074] The preparation of pea ACE inhibitory peptide consists of the following steps:

[0075] (1) Take pea protein, add distilled water according to the ratio of material to liquid 1g:10mL and mix evenly to obtain an aqueous solution of pea protein;

[0076] (2) Preheating the aqueous pea protein solution obtained in step (1) to 50°C, adding NaOH to adjust the pH to 7.0;

[0077] (3) Add flavor protease, the quality of flavor protease is 1.5% of the pea protein quality in step (1), at a temperature of 50°C and a pH of 7.0, carry out magnetic stirring enzymolysis for 6 hours, and the pH of the enzymolysis process is maintained at 7.0, Keep the temperature at 50°C to obtain pea protein hydrolyzate;

[0078] (4) Take the above-mentioned pea protein enzymatic hydrolyzate and inactivate the enzyme at 100°C for 15 minutes, and cool it with running water after inactivating the enzyme;

[0079] (5) Centrifuge the cooled enzymolysis solution at 4000 r / min for 15 minutes to obtain th...

Embodiment 2

[0081] The preparation of pea ACE inhibitory peptide consists of the following steps:

[0082] (1) Take pea protein, add distilled water according to the ratio of material to liquid 1g:10mL and mix evenly to obtain an aqueous solution of pea protein;

[0083] (2) Preheating the aqueous pea protein solution obtained in step (1) to 55°C, adding NaOH to adjust the pH to 7.0;

[0084] (3) Add composite protease, the quality of composite protease is 1.5% of the pea protein quality in step (1), at temperature 55 ℃, under the condition of pH 7.0, carry out magnetic stirring enzymolysis 6h, the pH of enzymolysis process is kept at 7.0, Keep the temperature at 55°C to obtain pea protein hydrolyzate;

[0085] (4) Take the above-mentioned pea protein enzymatic hydrolyzate and inactivate the enzyme at 100°C for 15 minutes, and cool it with running water after inactivating the enzyme;

[0086] (5) Centrifuge the cooled enzymolysis solution at 4000 r / min for 15 minutes to obtain the super...

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Abstract

The invention belongs to the technical field of active peptides, and particularly relates to a composite ACE inhibitory peptide with a synergistic effect and a preparation method and application thereof. The pea peptide and the corn peptide are compounded according to the ratio of 1: (0.75-1), and the ACE activity of the pea peptide and the ACE activity of the corn peptide are synergistic. The result of the embodiment shows that the IC50 value of the composite ACE inhibitory peptide is 0.547-0.636 mg/mL and is obviously superior to that of single pea peptide and single corn peptide under the same concentration, the composite ACE inhibitory peptide has good ACE inhibitory activity, the content of essential amino acid is high, the composite ACE inhibitory peptide can be better absorbed and utilized by human bodies, and the utilization rate of pea protein and corn protein is increased.

Description

technical field [0001] The invention belongs to the technical field of active peptides, and in particular relates to a composite ACE inhibitory peptide with synergistic effects and its preparation method and application. Background technique [0002] Hypertension is a major risk factor for the development of coronary heart disease, stroke, and end-stage renal disease. According to WHO statistics, the prevalence of hypertension among adults in the world is about 30%, and the prevalence in my country is about 17.5%. About 3 million people die of cardiovascular disease in my country every year, accounting for about 40% of the total mortality. It can be seen that the prevention and control of hypertension plays an extremely important role in the treatment of cardiovascular diseases. Angiotensin-converting enzyme (ACE; EC 3.4.15.1) is a zinc ion metallocarboxypeptidase that catalyzes the conversion of angiotensin I to angiotensin II, which constricts blood vessels and leads to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A23L2/385A23L2/52A23L2/60A23L2/66A23L33/18A61K38/01A61P9/12
Inventor 郑喜群张烁刘晓兰
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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