Medical use of triacetyl andrographolide as proinflammatory cytokine inhibitor
An inflammatory cytokine and andrographolide technology is applied in the field of medical use of triacetylandrographolide as an inflammatory cytokine inhibitor, and can solve the problem that the inhibitory effect and activity of triacetylandrographolide cannot be predicted. How to wait for the problem to achieve the effect of improving the inhibitory activity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0028] Preparation Example 1 Preparation of Andrographolide
[0029] Take 20 g of crude andrographolide, dissolve it in 450 mL of methanol at 72°C, let it stand for 24 hours and obtain white crystals by suction filtration, and dry it to obtain andrographolide.
Example Embodiment
[0030] Preparation Example 2 Preparation of triacetylandrographolide
[0031] 1g andrographolide dissolved in 10mLAc 2 O, heated to reflux for 2 hours, the reaction mixture was extracted with ethyl acetate, the organic layer was sequentially water, saturated NaHCO 3 Wash with saturated brine, anhydrous MgSO 4 dry. The white solid of triacetylandrographolide was obtained by silica gel column chromatography. The molecular formula is: C 26 H 36 O 8 .
[0032] The compound confirmation data is as follows:
[0033] (cm -1 ): 3454, 3080, 2954, 1731, 1683, 1375, 1250, 1191, 1075, 1026, 895, 606.
[0034] 1 H(400MHz, CDCl 3 ):δ
[0035] 7.00 (td, J = 6.84, 1.48 Hz, 1H, H-12), 5.92 (d, J = 5.96 Hz, 1H, H-14), 4.90 (bs, 1H, H-17a), 4.60 (dd, J = 11.68, 4.26 Hz, 1H, H-15a), 4.55 (dd, J = 11.24, 6.08 Hz, 1H, H-15b), 4.53 (bs, 1H, H-17b), 4.36 (d, J = 11.80 Hz , 1H, H-19a), 4.25 (dd, J = 11.24, 1.80 Hz, 1H, H-3), 4.12 (d, J = 11.80 Hz, 1H, H-19b), 2.52~2.37 (m, 3H) , 2.12 (s, 3H), 2.05 (s, 6...
Example Embodiment
[0039] Preparation Example 3 Preparation of 3,19-diacetylandrographolide, 14-acetylandrographolide, and 14,19-diacetylandrographolide
[0040] Add 5 g of andrographolide to 30 mL of acetic acid and stir and heat to 80° C., and react overnight. Extracted with ethyl acetate, the organic layer was washed with water and saturated brine successively, anhydrous MgSO 4 dry. Column chromatography obtained 3,19-diacetylandrographolide (AP11), 14,19-diacetylandrographolide (AP12), and 14-acetylandrographolide (AP13).
[0041]
[0042] The AP11 compound confirmation data is as follows:
[0043] Molecular formula: C 24 H 34 O 7 (434)
[0044] 1 H(400MHz, CDCl 3 ): 6.97 (1H, m, H-12), 5.03 (1H, d, J=5.6 Hz, H-14), 4.92 (1H, s, H-17), 4.62 (1H, s, H-17) , 4.59 (1H, dd, J = 11.2, 4.8 Hz, H-3), 4.47 (1H, dd, J = 10.4, 6.0 Hz, H-15), 4.38 (1H, d, J = 11.6 Hz, H- 19), 4.26 (1H, dd, J = 10.4, 2.0 Hz, H-15), 4.11 (1H, d, J = 12.0 Hz, H-19), 2.05 (6H, s, CH 3 CO-), 1.04 (3H, s, H-18), 0.78 (3H, s, H...
PUM
Property | Measurement | Unit |
---|---|---|
The average particle size | aaaaa | aaaaa |
The average particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap