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ACE inhibitory peptide in fermented milk and preparation method thereof

A technology of fermented milk and inhibitory peptides, which is applied in the field of enzyme inhibitory peptides and its preparation, can solve the problems of high production conditions, expensive equipment, complex production processes, etc., and achieve low production conditions, high food safety, and production The effect of simple process

Inactive Publication Date: 2009-11-04
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention aims to solve the problems that the current chemical ACE inhibitors have large side effects, and the biological ACE inhibitors have high requirements for production conditions, complex production processes, expensive equipment and bitter taste due to the use of biological enzymes

Method used

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  • ACE inhibitory peptide in fermented milk and preparation method thereof
  • ACE inhibitory peptide in fermented milk and preparation method thereof
  • ACE inhibitory peptide in fermented milk and preparation method thereof

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specific Embodiment approach 1

[0013] Specific embodiment 1: The ACE inhibitory peptide in fermented milk in this embodiment is mainly composed of four small molecular peptides whose amino acid sequences are KPAGDF, KAALSGM, KKAAMAM and LDHVPGGAR.

[0014] The ACE inhibitory peptide in the fermented milk of this embodiment can be prepared by the method of the present invention; the ACE inhibitory peptide in the fermented milk of this embodiment also includes a small amount of other small molecular polypeptides (such as figure 1 Shown), its dry matter content <10%.

[0015] The ACE-inhibiting peptide in the fermented milk of the present embodiment can also be directly divided into 30%~40%: 30%~40%: 10%~20%: 10 % to 20% by mass, including unavoidable impurity polypeptides.

[0016] The dry matter content of the ACE-inhibiting peptide in the dried fermented milk of this embodiment is >90%, the salt content is between 0% and 1.6%, and there is no bitter taste.

specific Embodiment approach 2

[0017] Specific embodiment two: the ACE inhibitory peptide in the fermented milk of this embodiment is prepared according to the following steps: 1. Lactobacillus helveticus (Lactobacillus helveticus) L03-1 and Lactobacillus casei (Lactobacillus casei) SL5 are inoculated in a protein concentration of 11%~ 12% skim milk, and then fermented at 35 to 39°C for 36 to 72 hours to obtain fermented milk; 2. Centrifuge the fermented milk for 30 minutes at a speed of 4000r / min, take the supernatant and filter, and the filtrate is Inactivate the enzyme at ±1°C for 10-15 minutes, then centrifuge again at 4000r / min for 30 minutes, then filter the supernatant with an ultrafiltration membrane to obtain the permeate; 3. Freeze-dry the permeate to obtain ACE-inhibiting peptides in fermented milk; wherein, the inoculum size of Lactobacillus helveticus L03-1 in step 1 is 4×10 8 ~8×10 8 cfu / L, the inoculation amount of Lactobacillus casei SL5 was 4×10 8 ~8×10 8 cfu / L; the ultrafiltration membr...

specific Embodiment approach 3

[0020] Embodiment 3: The difference between this embodiment and Embodiment 2 is: in step 4, the freeze-drying controls the moisture content to <5%. Other steps and parameters are the same as those in Embodiment 2.

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Abstract

The invention provides an ACE inhibitory peptide in fermented milk and the preparation method thereof, which relates to the inhibitory peptide of an enzyme and the preparation method thereof. The invention aims at solving the problems that the existing chemical ACE inhibitor has severe side effect, and due to the adoption of a biological enzyme method, biological ACE inhibitor has high requirements for production conditions, complicated production process, expensive equipment cost and bitter product. The ACE inhibitory peptide in the fermented milk mainly comprises four kinds of small molecular peptide the amino acid sequences of which are KPAGDF, KAALSGM, KKAAMAM and LDHVPGGAR. The invention has the preparation: 1. fermented milk preparation; 2. filtration, enzyme extermination, centrifugation and ultrafiltration; 3. refrigeration and desiccation. The ACE inhibitory peptide in the fermented milk is milk protein peptide with biological activity, which has no side effect on a human body, and the ACE inhibitory peptide is edible and highly secure without any bitterness; the ACE inhibitory peptide can withstand high temperature of 90 DEG C without degeneration. The method for preparing the ACE inhibitory peptide in the fermented milk has the advantages of low requirements for production conditions, simple production process, no need for expensive equipment, low cost, high security, no bitter product, and the like.

Description

technical field [0001] The invention relates to an enzyme inhibitory peptide and a preparation method thereof. Background technique [0002] Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptide carboxypeptidase, which exists in vascular endothelial cells or epithelial cells of various tissues and body fluids such as plasma and urine; it has peptide bond specificity Reactivity requires anion catalysis, among which chloride ion is the most effective activator. ACE has the function of regulating blood pressure in the human renin-angiotensin system (Renin angiotensin system, RAS) and kallikrein-kinin system (Kallikrein-kinin system, KKS), and the substrate of ACE (especially the natural substrate Angiotensin I and bradykinin in drugs) will induce ACE to produce vasoconstriction activity, resulting in increased blood pressure and endangering human health. [0003] Inhibition of ACE has become another effective way to control the rise of blood pressure. However, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/34C12R1/225C12R1/245
Inventor 霍贵成姜瞻梅田波
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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