Application of rhizoctonia solani mycelium to improving trichodermin in trichoderma harzianum

A technology of Rhizoctonia solani and Trichoderma harzianum, applied in the field of microorganisms, can solve the problems of low production capacity and low yield level, and achieve a reliable effect of increasing the yield level

Inactive Publication Date: 2008-10-01
CHINA JILIANG UNIV
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Problems solved by technology

Wild-type strains isolated from nature often have l

Method used

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Effect test

Embodiment 1

[0020] The cultivation of Rhizoctonia solani: culture medium is potato dextrose liquid medium (PD), 300mL triangular bottle packs PD liquid medium 60mL, picks a little Rhizoctonia solani mycelium in the sterilized culture bottle with inoculation hook, Place on a shaker at 26°C to 28°C, 200r / min, and shake for 6 days. The fermentation broth is centrifuged at 5000r / min for 10min, and the mycelium is collected. Vacuum freeze-drying at -53°C and 0.5mbar for 15h, the inactivated bacteria Grind the filaments into small particles for later use;

[0021] Liquid fermentation of Trichoderma harzianum: Trichoderma harzianum is an endophytic fungus isolated from Chinese wolfberry. This strain has been preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms. The preservation date was August 14, 2006, and the preservation registration number was CGMCC No. .1780 see the publication of patent application 200710066843.1, the publication number is CN...

Embodiment 2

[0025] The cultivation of Rhizoctonia solani: with embodiment 1;

[0026] Liquid fermentation of Trichoderma harzianum: Trichoderma harzianum is an endophytic fungus isolated from Chinese wolfberry. This strain has been preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms. The preservation date was August 14, 2006, and the preservation registration number was CGMCC No. .1780 see the publication of patent application 200710066843.1, the publication number is CN101037656. Fermentation medium formula: its components and content (mass contained in each liter of medium) are 90 mg of small particles of Rhizoctonia solani mycelium, 30 g of peptone, 10 g of beef extract, 24 g of glucose, MnCl 2 0.01g, KH 2 PO 4 0.1g, MgSO 4 0.05g; no Rhizoctonia solani mycelium was added in the control, and the remaining components and contents were the same as the fermentation medium formula; 60mL of fermentation medium in a 300mL triangular bottle w...

Embodiment 3

[0030] The cultivation of Rhizoctonia solani: with embodiment 1;

[0031] Liquid fermentation of Trichoderma harzianum: Trichoderma harzianum is an endophytic fungus isolated from Chinese wolfberry. This strain has been preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms. The preservation date was August 14, 2006, and the preservation registration number was CGMCC No. .1780. Fermentation medium formula: its component and content (mass contained in every liter of medium) are 90mg of small particles of Rhizoctonia solani mycelium (added after 24 hours of Trichoderma harzianum liquid fermentation), peptone 30g, beef Extract 10g, glucose 24g, MnCl 2 0.01g, KH 2 PO 4 0.1g, MgSO 4 0.05g; no Rhizoctonia solani mycelium was added in the control, and the remaining components and contents were the same as the fermentation medium formula; 60mL of fermentation medium in a 300mL triangular bottle was used to draw 1mL of Trichoderma harzi...

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Abstract

The present invention provides use of rhizoctonia solani mycelium in the course of improving trichodermin level generated by Trichoderma harzianum, belonging to microbial technology field. The invention enhances the concentration of the trichodermin in the broth by adding inactivated rhizoctonia solani mycelium in the Trichoderma harzianum culture medium.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a method for increasing the toxin production level of Trichoderma harzianum. Background technique [0002] The titer in microbial fermentation is often the bottleneck that determines the production cost and realizes industrial production. Genetic modification of strains, optimization of culture medium and culture conditions are common methods to improve the production level. In recent years, fungal elicitors have been widely used in the research of plant cell culture and microbial fermentation to produce secondary products. The research team isolated and screened an active endophytic fungus, Trichoderma harzianum, that produces Trichodermin from Lycium chinensis. Trichoderma has a strong inhibitory effect on a variety of crop pathogenic fungi, and has broad application prospects in the control of crop diseases. Wild-type strains isolated from nature often have low levels of h...

Claims

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Application Information

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IPC IPC(8): C12P7/62C12R1/885
Inventor 俞晓平申屠旭萍董胜张王为民
Owner CHINA JILIANG UNIV
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