Method for preparing recombination porcine alpha-type interferon

Abstract

The invention discloses a method for preparing porcine recombinant IFN-alpha. The method is to use a special primer - PCR to amplify target genes of the porcine IFN-alpha, to connect the target genes with an expression vector - pET-28a and an expression vector - pGEX-5X, to transform colon bacillus, to make the colon bacillus highly efficiently express the porcine IFN-alpha and to prepare porcine recombinant IFN-alpha freeze-dried injection through the techniques such as high purification, sterilization, split charging, freeze-drying and so on. The method is shown to have obvious treatment effect on porcine virus diarrhea, influenza and so on and be superior to similar products in the aspects of safety and cure rate through safety experiment, IFN efficacy inspection and clinic probation. The preparation technique is simple; industrialized production is easy; transportation and preservation are easy after freeze-drying. After the porcine recombinant IFN-alpha is injected into a porcine body, the porcine recombinant IFN-alpha is characterized by good healing effect, reliable assembly, no side effect like inocuity and so on, can be used for curing diseases like the porcine virus diarrhea, the influenza and so on, and is novel high-efficient safe nonpersistent broad-spectrum antiviral preparation.

Description

technical field [0001] The invention relates to a preparation method and process of recombinant porcine α-type interferon (interferon, IFN). It is made by transforming the recombinant plasmid with porcine α-IFN gene into Escherichia coli, and making it highly express porcine α-IFN. After purification, it is made by adding appropriate amount of porcine plasma albumin stabilizer and lyophilized. It is used for the treatment of porcine viral diarrhea and epidemics. Viral diseases such as influenza. The standards for development and verification are all using the national SFDA-certified recombinant human α-IFN (China Biological Products Regulations, China Biological Products Standardization Committee, edition 2000) as a reference and implemented. Background technique [0002] Porcine viral disease is the main infectious disease that seriously restricts the healthy development of pig breeding industry. Occurs throughout the year, such as swine viral diarrhea and influenza. Onc...

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Problems solved by technology

[0004] At present, porcine white blood cell IFN is the first to be used in veterinary clinics in China. However, the cost of white blood cell IFN obtained by blood cell separation is relatively high, and the blood source is easily polluted, making it difficult to carry out industrial production.