Sea bacillus subtilis and screening method thereof

A technology of Bacillus subtilis and a screening method, applied in the field of marine Bacillus subtilis and its screening, can solve the problems of lack of ideal control technology, soil poisoning, accumulation of toxic substances, etc. The effect of high screening yield

Inactive Publication Date: 2008-11-05
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

In the past five years, enough attention has been paid to the increase of fungal pathogens and the accumulation of toxic substances after continuous planting of pure Chinese fir forests, causing soil poisoning, soil fertility decline, and severe blight of Chinese fir seedlings, but there is no ideal and targeted solution. It is urgent to have new biocontrol technology as soon as possible to solve the problems of soil diseases and soil fertility decline in Chinese fir plantations

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  • Sea bacillus subtilis and screening method thereof

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Embodiment Construction

[0025]The present invention adopts the method that conventional flat plate mutual antagonism screening model and directional screening combine, and its concrete steps: 1. collect the juvenile supporting roots (with Elasticity, the sieve tube fiber is hollow, like a sponge, and has the effect of inflating and holding oxygen. The old roots are not taken, because the old roots have gradually lignified and hardened.) Samples with soil; ②After being treated at 85°C for 15 minutes , and isolate the required strains by the plate dilution method; ③Take the main harmful fungus Fusarium oxysporum wilting type in the rhizosphere soil of Chinese fir plantation as the target, and screen out the surface soil of the root surface of the plant with high antagonistic activity through the plate antagonist test The marine Bacillus subtilis isolated from the sample; ④Through the pot test, the ones with high antagonistic activity and the ability to colonize in the rhizosphere soil of Chinese fir see...

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Abstract

The invention relates to an application technology of marine microorganism and a disease control method for fungi in the rhizosphere soil in Chinese fir plantation, in particular to a type of marine bacillus subtilis and a screening method thereof. The microorganism refers to the bacillus subtilis 3728C, the number for preservation of which is CCTCC No. M207052. The method combines a screening model with mutually antagonized plates and directional screening. By the screening method, strains of the marine bacillus subtilis for controlling the harmful fungi in the rhizosphere soil in Chinese fir plantation are used as one of the major components to develop a type of disease-preventing and toxicity-decreasing ecological agent, which solves the problems of continuous planting of Chinese firs. The disease-preventing and toxicity-decreasing ecological agent is a type of microbial preparation with high efficiency, safe and free of toxicity and residues, which can enhance the development of the green ecosystem of the Chinese fir plantation. With satisfactory application and test results in Hunan Huitong Chinese Fir Plantation, the disease-preventing and toxicity-decreasing ecological agent has bright development prospects.

Description

technical field [0001] The invention relates to marine microbial application biotechnology and the prevention and treatment of fungal diseases in the rhizosphere soil of Chinese fir, in particular to a marine bacillus subtilis and a screening method thereof for preventing and treating the fungal diseases in the rhizosphere soil of Chinese fir plantations. Background technique [0002] Chinese fir (Cunninghamia lanceolata) is a unique fast-growing and high-yielding timber tree species in my country. It is distributed in 17 provinces, municipalities, and autonomous regions in southern China. The existing fir plantation area is 12.1407 million hectares, accounting for about 24% of the total area of ​​my country's plantation forest. Studies over the years have shown that: with the increase of the number of continuous planting generations of Chinese fir, the productivity of Chinese fir will decline, and obstacles to continuous planting of Chinese fir will appear. Continuous plant...

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Application Information

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IPC IPC(8): C12N1/20C12R1/125
Inventor 胡江春徐洪利刘丽薛德林马成新汪思龙王书锦
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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