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Method for fast measuring dispersion coefficient of matter in liquid phase by capillary electrophoresis apparatus

A technology of capillary electrophoresis and diffusion coefficient, applied in the direction of electrical digital data processing, instruments, measuring devices, etc., can solve the problems of complex data processing and lack of versatility

Inactive Publication Date: 2008-12-10
INST OF CHEM CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the data processing of this method is more complex and lacks versatility

Method used

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  • Method for fast measuring dispersion coefficient of matter in liquid phase by capillary electrophoresis apparatus
  • Method for fast measuring dispersion coefficient of matter in liquid phase by capillary electrophoresis apparatus
  • Method for fast measuring dispersion coefficient of matter in liquid phase by capillary electrophoresis apparatus

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Embodiment Construction

[0061] Take a capillary provided by a manufacturer with a radius of 37.5 μm and a length of 60 cm (the distance from the injection end to the detection window is 50 cm) as an example to illustrate the selection of measurement conditions. Use L-phenylalanine (Mcgraw in its book " American institute of physics handbook ") value of diffusion coefficient of L-phenylalanine at 25 ℃ is 7.06 * 10 -6 cm 2 / s) as an example. Objects and methods of the present invention can be better understood from the following detailed description in conjunction with the accompanying drawings.

[0062] (1) Cleaning of fused silicon capillary. Clean the capillary as follows: methanol for 15 minutes, water for 10 minutes, 1mol / L nitric acid for 15 minutes, water for 10 minutes, 1mol / L sodium hydroxide for 15 minutes, and finally water for 10 minutes. Between every two measurements, the capillary was rinsed with 1 mol / L sodium hydroxide for 5 minutes, and then rinsed with water and carrier solution f...

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Abstract

The invention relates to a method for using a capillary electrophoresis instrument to detect the diffusion coefficients of a substance in a liquid phase. The invention discloses a plurality of key factors which affect the accuracy of the detection when using the capillary electrophoresis instrument to detect the diffusion coefficients of the substance, then further provides a fast detecting method which is used for the diffusion coefficients of the substance with a certain commonality and can detect the diffusion coefficients of the substance to be detected in water or an organic solvent. The method of the invention associates the diffusion coefficients of the substance with the appearance time of the elution peak of the substance as well as the width of a self-peak and can fast calculate and obtain the diffusion coefficient value of the substance.

Description

technical field [0001] The invention relates to a method for measuring the diffusion coefficient of a substance in a liquid phase with a capillary electrophoresis apparatus. Background technique [0002] Diffusion is the process by which molecules, ions, or other small particles spontaneously mix and move from areas of relatively high concentration to areas of low concentration. The widely accepted one is to describe diffusion in terms of the diffusion coefficient, which is used to describe the rate of change of concentration between two points at a certain distance. [0003] The slow rate of diffusion underscores its importance. In most cases, diffusion occurs sequentially with other phenomena, and when it is the slowest step in the process, it becomes the determinant of the whole process. Diffusion, for example, often limits the efficiency of distillation and the reaction rate of porous catalysts in industry, and can also control the growth rate of penicillin-producing m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N13/00G01N27/447G06F19/00
Inventor 陈义左敏齐莉
Owner INST OF CHEM CHINESE ACAD OF SCI
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